| Edge RBR ITC Limited | Information & communication | 2025 |
| EDI Zone UK Ltd | Professional & scientific | 2020 |
| Edison Lighthouse Limited | Arts & recreation | 2024 |
| EDS Consultants Ltd | Professional & scientific | 2015 |
| EDS34 Limited | Hospitality | 2026 |
| Edspace Ltd | Construction | 2013 |
| Eduard Nicholas Limited | Construction | 2026 |
| Education Group Limited | Education | 2001 |
| Education Workshops Limited | Extraterritorial | 2001 |
| Eduplus Ltd | Professional & scientific | 2017 |
| Edward Building Developments Ltd | Construction | 2015 |
| EEO Care Limited | Health & social care | 2017 |
| EEQ Limited | Wholesale & retail | 2024 |
| Efa's Care Limited | Health & social care | 2022 |
| Efficiency Secured Limited | Financial services | 2022 |
| Efnineone Ltd | Transport & logistics | 2024 |
| Eggfree Cake Inn Ltd | Wholesale & retail | 2018 |
| Eglo UK Limited | Manufacturing | 2001 |
| EHQ Services Ltd | Health & social care | 2024 |
| Eight Development Limited | Construction | 2009 |
| Eight Estate Management Limited | Real estate | 2019 |
| Eight Residential Ltd | Real estate | 2020 |
| EIGHT6 Roofing Services Ltd | Construction | 2023 |
| Eikya Consultancy Ltd | Health & social care | 2020 |
| EJ Physiotherapy Ltd | Health & social care | 2025 |
| EK Legal Consultancy Ltd | Other services | 2020 |
| Ekaya Limited | Professional & scientific | 2023 |
| EKB ROP Ltd | Hospitality | 2025 |
| EKS Associates Limited | Professional & scientific | 2015 |
| EL Sayed Cars Limited | Transport & logistics | 2015 |
| Elebiv Ltd | Professional & scientific | 2025 |
| Electric Gate Centre Limited | Construction | 1991 |
| Electric Motor Rewinds Limited | Extraterritorial | 2006 |
| Electric Motor Sales Limited | Extraterritorial | 2006 |
| Electric Motor Services Limited | Extraterritorial | 2006 |
| Electric Motors Ltd | Manufacturing | 2004 |
| Electro Engineering Ltd | Construction | 2022 |
| Electrowill Design Limited | Information & communication | 2009 |
| Elegant Mart Ltd | Wholesale & retail | 2024 |
| Elemoto Limited | Extraterritorial | 2006 |
| Elephant and INK Creative Ltd | Administrative & support | 2022 |
| Elh.Io Ltd | Information & communication | 2026 |
| Elijah Composites Ltd | Information & communication | 2026 |
| Elite (London) Living Ltd | Real estate | 2019 |
| Elite Airport Rides Ltd | Transport & logistics | 2024 |
| Elite Carpentry and Building Solutions Ltd | Construction | 2022 |
| Elite Cleaning Services 13 Limited | Administrative & support | 2021 |
| Elite Cooling Solutions Ltd | Construction | 2008 |
| Elite Feet Podiatry Limited | Health & social care | 2021 |
| Elite Football League Ltd | Arts & recreation | 2026 |
| Elite Sports Coaching TW Ltd | Other services | 2018 |
| Elizabeth Parker Promotions Limited | Wholesale & retail | 1995 |
| Elize Estates Ltd | Real estate | 2026 |
| Ella International Transport Ltd | Transport & logistics | 2026 |
| Ellbeck Adventure Ltd | Arts & recreation | 2025 |
| Elliott+Lily Ltd | Manufacturing | 1999 |
| Ellis’S Construction Services Ltd | Construction | 2025 |
| Ellsworth Dispensing Solutions Limited | Extraterritorial | 2018 |
| Elly Ltd | Transport & logistics | 2017 |
| Elmleigh Properties Limited | Financial services | 2025 |
| Eloque Properties Limited | Real estate | 2024 |
| ELR22 Ltd | Information & communication | 2020 |
| EMA Locks 24/7 Ltd | Administrative & support | 2026 |
| Embankment Properties UK Limited | Real estate | 2024 |
| Embankment Stores Limited | Wholesale & retail | 2014 |
| Emedam Ltd | Construction | 2025 |
| Emegeo Limited | Hospitality | 2025 |
| Emerald Build Limited | Construction | 2018 |
| Emfast Limited | Wholesale & retail | 2007 |
| EMI Coffee Ltd | Hospitality | 2026 |
| Emilia.Md Limited | Manufacturing | 2016 |
| Emilyjonesretail Ltd | Wholesale & retail | 2025 |
| Emmakot Ltd | Wholesale & retail | 2022 |
| Empire Eats Limited | Hospitality | 2024 |
| Emplas Commercial (North) Ltd | Construction | 2024 |
| Emplas Overseas Ltd | Extraterritorial | 2011 |
| Emplas Window Systems Limited | Manufacturing | 1985 |
| Employability Pathways CIC | Education | 2025 |
| Empower Healthcare Services Ltd | Health & social care | 2015 |
| EMR 1 Ltd | Hospitality | 2024 |
| EMS Inspection Solutions Limited | Financial services | 2014 |
| EMT Automotive UK Ltd | Wholesale & retail | 2024 |
| Emunah Innovations Ltd | Wholesale & retail | 2019 |
| Enable Living Solutions Limited | Health & social care | 2020 |
| Enabling Independence Living Ltd | Health & social care | 2026 |
| Enabling Independent Living Ltd | Health & social care | 2026 |
| ENB Recruitment and Training Limited | Administrative & support | 2016 |
| Endio & Aren Property Investment Ltd | Real estate | 2024 |
| Enene Ltd | Information & communication | 2020 |
| Energy Securities Limited | Professional & scientific | 2015 |
| ENG Engineering Solutions Ltd | Construction | 2022 |
| Enhance My Kitchen Ltd | Wholesale & retail | 2020 |
| Enriched Leadership Limited | Professional & scientific | 2024 |
| Enrolling Ltd | Education | 2014 |
| Entps Trading Limited | Wholesale & retail | 2024 |
| Entrepid Properties Limited | Real estate | 2024 |
| Entrepid Services Limited | Wholesale & retail | 2019 |
| Envision Exhibitions Ltd | Manufacturing | 2025 |
| Epic Kebab Ltd | Hospitality | 2026 |
| Equinox Automation & Controls Limited | Information & communication | 2002 |