| Electra Technology Limited | Administrative & support | 1996 |
| Electrics by Ebers Ltd | Energy | 2014 |
| Electronic Audio Systems Limited | Construction | 1988 |
| Elements Builds and Renovations Ltd | Construction | 2022 |
| Elenchus Consulting Ltd | Information & communication | 2006 |
| Elesel (Kent) Limited | Construction | 2026 |
| Elevetix Limited | Professional & scientific | 2022 |
| Elite Distribution Services Limited | Transport & logistics | 2024 |
| Elite Gardens and Landscapes Ltd | Professional & scientific | 2020 |
| Elite Healthcare Developments Ltd | Construction | 2025 |
| Elite Interactive Ltd | Arts & recreation | 2024 |
| Elite Sailing (Adventure and Training) Ltd | Arts & recreation | 2026 |
| Elix Fragrance Limited | Manufacturing | 2023 |
| Elizabeth Whitehead Limited | Administrative & support | 2011 |
| ELK IT Ltd | Information & communication | 2015 |
| Elkington Development Limited | Real estate | 1993 |
| Ellamy Consultancy Services Ltd | Professional & scientific | 2020 |
| Elle-Evate Hair Ltd | Other services | 2025 |
| Elliot Leech Properties Limited | Real estate | 2015 |
| Ellipsys Associates Ltd | Financial services | 2013 |
| Ellis & SON Vehicle Builders Limited | Manufacturing | 1964 |
| ELM Fencing Ltd | Construction | 2007 |
| Elmfield Management Company Limited | Households | 2009 |
| Elmhurst Bloodstock Limited | Financial services | 1987 |
| Elneny Academy Ltd | Arts & recreation | 2023 |
| Elora Wellness Ltd | Arts & recreation | 2025 |
| ELR Talent Consulting Ltd | Professional & scientific | 2019 |
| Elsynge ST. Mary Ltd | Real estate | 2022 |
| Elszew Limited | Health & social care | 2018 |
| Elvis Theodosiou Ltd | Professional & scientific | 2018 |
| Elwood Group Limited | Hospitality | 2024 |
| EM Crafts Limited | Manufacturing | 2024 |
| Emanuel Bespoke Renovations Ltd | Construction | 2025 |
| Emarketing and Solutions Limited | Administrative & support | 2025 |
| Emarky Ltd | Wholesale & retail | 2026 |
| Emerald Tyre & Wheel Solutions UK Ltd | Wholesale & retail | 2019 |
| Emerson Works Ltd | Health & social care | 2019 |
| Emil Go Ltd | Education | 2018 |
| Emily Allen Copywriting Ltd | Professional & scientific | 2023 |
| Emmadoc Ltd | Health & social care | 2020 |
| Emmy Cars Limited | Wholesale & retail | 2021 |
| Emporium of Cool Ltd | Wholesale & retail | 2020 |
| Enableuk Recruitment Ltd | Professional & scientific | 2022 |
| Enaible AI Ltd | Professional & scientific | 2024 |
| Enchanter Press Limited | Information & communication | 2021 |
| Endeva Supplies Limited | Wholesale & retail | 2013 |
| Endocrine Medical and Surgical Care Ltd | Health & social care | 2019 |
| Endorphin Capital Limited | Professional & scientific | 2010 |
| Eneji Integrated Farms Ltd | Manufacturing | 2024 |
| Energon Connections Ltd | Construction | 2021 |
| Energy Live News Limited | Administrative & support | 2010 |
| Energy PS Limited | Professional & scientific | 2022 |
| Enerjee Limited | Professional & scientific | 2020 |
| Engage Search Limited | Administrative & support | 2010 |
| Engine NO 42 Limited | Arts & recreation | 2024 |
| England Korfball Ltd | Education | 2021 |
| English Schools Integration Ltd | Education | 2025 |
| English's of Brighton Limited | Administrative & support | 2006 |
| English's Oyster Bar Limited | Real estate | 1973 |
| Enhance Electrical Solutions Ltd | Construction | 2024 |
| Enlab Limited | Information & communication | 2025 |
| Enlightened Minds Therapy Ltd | Other services | 2020 |
| ENO Eco Ltd | Wholesale & retail | 2020 |
| ENP Professionals Limited | Health & social care | 2014 |
| ENT Matters Ltd | Health & social care | 2018 |
| Entrepreneur Insight Limited | Professional & scientific | 2018 |
| Enviro-Cool Commercial Limited | Manufacturing | 2021 |
| Enviro-Cool Global Ltd | Wholesale & retail | 2024 |
| Enviro-Cool International Ltd | Wholesale & retail | 2015 |
| Enviro-Cool UK Limited | Manufacturing | 2007 |
| Environer Ltd | Extraterritorial | 2023 |
| Envis Media Ltd | Professional & scientific | 2020 |
| Envision Economics Limited | Professional & scientific | 2014 |
| Envision Partnership Ltd | Professional & scientific | 2015 |
| Eonic Digital Group Ltd | Arts & recreation | 2012 |
| EP Cardiology Consulting Ltd | Health & social care | 2022 |
| EPD Properties Limited | Real estate | 2018 |
| Epi-Vision Limited | Administrative & support | 2018 |
| Epic Engineering UK Limited | Manufacturing | 2004 |
| Epicrent Investment Limited | Real estate | 2024 |
| EPP Kitchens and Bathrooms Ltd | Construction | 2005 |
| Epsa Limited | Health & social care | 2010 |
| Epsilon House Management Limited | Real estate | 2003 |
| EQ8 Services Ltd | Construction | 2025 |
| Equestrian and Country Properties Limited | Real estate | 2000 |
| Equus Country and Equestrian Limited | Real estate | 2014 |
| Equus Country and Equestrian Properties Limited | Real estate | 2014 |
| Equus International Property Limited | Real estate | 1999 |
| Equus Property Ltd | Real estate | 2001 |
| Erdal Gents Ltd | Other services | 2020 |
| Eridge Property Limited | Real estate | 2025 |
| Erika Language Services Limited | Professional & scientific | 2013 |
| Eris Contractors Ltd | Construction | 2019 |
| ERM Drilling Services Limited | Construction | 2019 |
| Ernest West & Beynon,Limited | Other services | 1909 |
| ESM Uckfield Lodge Ltd | Real estate | 2026 |
| ESP PR Limited | Information & communication | 2003 |
| Especially for YOU Parties Ltd | Arts & recreation | 2014 |
| Esports Ventures Limited | Information & communication | 2022 |
| Essendon Transport Services Ltd | Transport & logistics | 2024 |