| Essentialnet Limited | Information & communication | 2005 |
| Essex & Herts Haematology Limited | Health & social care | 2021 |
| Estate and Field Management Limited | Administrative & support | 2002 |
| Estudio Diseno Ltd | Professional & scientific | 2023 |
| Eternal IT Solutions Limited | Information & communication | 2010 |
| Eternal Systems Limited | Information & communication | 2005 |
| Ethos Resourcing Solutions Limited | Administrative & support | 2012 |
| Euromed Properties Limited | Real estate | 2002 |
| European Public Policy Associates (UK) Limited | Professional & scientific | 2002 |
| European Supply Chain Services Limited | Administrative & support | 2025 |
| Euroweb (S.W.) Limited | Wholesale & retail | 1989 |
| Euvira Padel Ltd | Arts & recreation | 2025 |
| Evans Carpentry & Construction Ltd | Construction | 2025 |
| Evasubs Ltd | Hospitality | 2024 |
| Evelina Ltd | Real estate | 2025 |
| Evergreen Innovations Limited | Professional & scientific | 2016 |
| Everprize Ltd | Professional & scientific | 2022 |
| Evisualise Ltd | Information & communication | 2021 |
| Evolution Electrical Construction (UK) Limited | Construction | 2014 |
| Evolution Environmental Limited | Professional & scientific | 2010 |
| Evolution Racing Ltd | Other services | 2020 |
| Evolution Tennis Limited | Arts & recreation | 2015 |
| Evolver Property Ltd | Construction | 1996 |
| Excel Laser Profilers Ltd | Extraterritorial | 2023 |
| Excellence EYE Care I&C Ltd | Health & social care | 2023 |
| Exclusive Angels Cleaning Company Ltd | Administrative & support | 2025 |
| Exhibition Freighting Limited | Transport & logistics | 1981 |
| Exitar Limited | Households | 2003 |
| Exito Consulting Ltd | Information & communication | 2018 |
| Express European Couriers Ltd | Transport & logistics | 1999 |
| Express Medical Services Logistics Ltd | Transport & logistics | 2025 |
| Express Services (Beckenham) Limited | Construction | 2007 |
| Extreme Ventures Ltd | Education | 2019 |
| Eyad 2017 Fish and Chips Shop Ltd | Hospitality | 2021 |
| EYE GEN Ltd | Health & social care | 2022 |
| Eyretec Limited | Professional & scientific | 2012 |
| Ezy-Clean Windows Limited | Other services | 2001 |
| F & C Electrical Ltd | Manufacturing | 2004 |
| Fabric London Limited | Construction | 2013 |
| Fabrica Trading Limited | Other services | 2018 |
| Fade Nation Ltd | Other services | 2025 |
| Fairhill Consulting Ltd | Professional & scientific | 2024 |
| Fairhill Spirits Ltd | Manufacturing | 2024 |
| Fairlawne Holdings Limited | Agriculture & fishing | 2019 |
| Fairlight Court Management Limited | Households | 1980 |
| Fairlight Media Consulting Ltd | Professional & scientific | 2019 |
| Famvault Ltd | Financial services | 2025 |
| Farmer Palmer's Products Limited | Manufacturing | 2008 |
| Farmhouse Property Limited | Professional & scientific | 2001 |
| Farrell Limited | Construction | 2025 |
| Farren Finance Ltd | Financial services | 2021 |
| Farry Ltd | Wholesale & retail | 2024 |
| Fashion Delivered Ltd | Professional & scientific | 2018 |
| Fast Giraffe Limited | Professional & scientific | 2011 |
| Fastlanemoto Limited | Wholesale & retail | 2011 |
| Fasttrack VC General Partner LLP | | 2017 |
| Fastway Agency Ltd. | Construction | 2004 |
| FAT Trout Films LLP | | 2017 |
| Fausto It's Real Productions Limited | Information & communication | 2018 |
| Faversham Counselling Service Limited | Health & social care | 2012 |
| Fawz Imaging Limited | Health & social care | 2012 |
| Fblb Catering Ltd | Hospitality | 2024 |
| FC Plus Limited | Professional & scientific | 2012 |
| FCC Consulting Ltd | Professional & scientific | 2025 |
| FD Direct Wealth Management Ltd | Financial services | 2023 |
| FDF Agriculture Limited | Manufacturing | 2025 |
| Fearfixer Limited | Information & communication | 2025 |
| Fearless Self Defence Ltd | Arts & recreation | 2017 |
| Febo Designs Limited | Arts & recreation | 2015 |
| Federation of Independent Practitioner Organisations | Other services | 2001 |
| Felicity Francis Limited | Arts & recreation | 2015 |
| Felro Homes Ltd | Real estate | 2024 |
| Fenchurch Risk Limited | Professional & scientific | 2026 |
| Fenner Paper Company Limited | Wholesale & retail | 1985 |
| Fenner Physio Ltd | Health & social care | 2026 |
| Feoffee Fields Management Company Ltd | Households | 2025 |
| Fernandezhouse Ltd | Real estate | 2022 |
| Ferncroft Collection Ltd | Wholesale & retail | 2023 |
| Fero Catering Limited | Hospitality | 2025 |
| FES Financial Engineering Solutions Ltd | Financial services | 2018 |
| FES Passepartout LLP | | 2025 |
| FF Group Holdings Limited | Financial services | 2025 |
| FFC Building Limited | Construction | 2021 |
| Fibersil Limited | Manufacturing | 2025 |
| Field 7 Ventures Limited | Administrative & support | 2023 |
| Field Management Ltd | Financial services | 2012 |
| Figure OUT Money Limited | Professional & scientific | 2018 |
| Figures Limited | Professional & scientific | 1996 |
| Filament Ventures Ltd | Financial services | 2021 |
| Filebox Ltd | Information & communication | 2007 |
| Filo Classical Limited | Information & communication | 2024 |
| Finabee Ltd | Education | 2024 |
| Final EYE Detailing Limited | Wholesale & retail | 2025 |
| Financial Engineering Solutions Ltd | Financial services | 2020 |
| Financial EYE Limited | Administrative & support | 2013 |
| Financial Modelling Studio Ltd | Information & communication | 2019 |
| Financially Fit Women Ltd | Education | 2014 |
| Findmeinsight Limited | Professional & scientific | 2018 |
| Findmykit Limited | Other services | 2012 |
| Finishing Matters Ltd | Professional & scientific | 2009 |