| Drapers Bakery Limited | Manufacturing | 2003 |
| Drawing With Light Ltd | Professional & scientific | 2025 |
| Draxs SE1 Limited | Hospitality | 2012 |
| Dream Big Explore CIC | Education | 2020 |
| Drehwerks Ltd | Wholesale & retail | 2026 |
| DRG Systems Ltd | Professional & scientific | 2022 |
| Drinkwater Construction Limited | Construction | 2005 |
| Drinkwater Construction LLP | | 2006 |
| Drmhl Medical Ltd | Health & social care | 2023 |
| Drunken Duck Distillery Ltd | Manufacturing | 2018 |
| DS Psychology Limited | Education | 2019 |
| DSG Groundworks Ltd | Construction | 2010 |
| DSP Enterprises Ltd | Administrative & support | 2024 |
| DTR Trading Ltd | Construction | 2001 |
| Dudfield Drylining Limited | Construction | 2001 |
| Dudley Infracare Developments Holdings Limited | Real estate | 2005 |
| Dudley Infracare Developments Limited | Real estate | 2005 |
| Dudley Infracare Lift (1) Limited | Real estate | 2005 |
| Dudley Infracare Lift Holdings (1) Limited | Real estate | 2005 |
| Dudley Infracare Lift Limited | Real estate | 2005 |
| Duffertek Ltd | Information & communication | 2010 |
| Duncan Hurley Ltd | Health & social care | 2022 |
| Dunlop GRG Holdings Limited | Professional & scientific | 1998 |
| Duro Builders Ltd | Construction | 2014 |
| Duro Construction Ltd | Construction | 2014 |
| Durrant and Paine Limited | Real estate | 2021 |
| Dwnb Properties Ltd | Real estate | 2021 |
| E L Brown Holdings Limited | Financial services | 2023 |
| E-Hub Services Ltd | Wholesale & retail | 2021 |
| E.L.Brown | Agriculture & fishing | 1961 |
| E.W.F. Edgwick Limited | Real estate | 1998 |
| E.Washbourne Limited | Construction | 1957 |
| E247 Limited | Information & communication | 2005 |
| EA Robinson Imaging Ltd | Health & social care | 2025 |
| Eae-Solutions Ltd | Professional & scientific | 2023 |
| East London Lift Accommodation Services Limited | Real estate | 2003 |
| East London Lift Accommodation Services NO2 Limited | Real estate | 2005 |
| East London Lift Holdco NO2 Limited | Real estate | 2005 |
| East London Lift Holdco NO3 Limited | Real estate | 2009 |
| East London Lift Holdco NO4 Limited | Real estate | 2009 |
| East London Lift Investments Limited | Real estate | 2003 |
| EB Livery Services Ltd | Agriculture & fishing | 2019 |
| Echo Digital Media Limited | Administrative & support | 2010 |
| Ecliptic Holdings Limited | Financial services | 2021 |
| ED Child and Adolescent Psychiatry Experts Limited | Transport & logistics | 2006 |
| Edgwicks Limited | Transport & logistics | 1995 |
| Edinburgh Medical Imaging Limited | Health & social care | 2024 |
| EDV Ltd | Professional & scientific | 2023 |
| Edward Oliver Merchandising Consultancy Ltd | Professional & scientific | 2021 |
| Edyoucate Ltd | Education | 2023 |
| EIS Midlands Limited | Energy | 2010 |
| Electro Clouds Limited | Wholesale & retail | 2016 |
| Electrolab Biotech Limited | Manufacturing | 2011 |
| Electronic Security and Fire Ltd | Administrative & support | 2023 |
| Electroplating Contract Services Ltd | Manufacturing | 2014 |
| Elite Growth Ltd | Professional & scientific | 2021 |
| Ellas NO3 Limited | Real estate | 2009 |
| Ellas NO4 Limited | Real estate | 2009 |
| Elmwood Barn Limited | Real estate | 2025 |
| Emberstone Ventures Limited | Financial services | 2024 |
| Eminent Surveyors Limited | Construction | 2020 |
| Emotive Design Ltd | Manufacturing | 2003 |
| Emsea Group Limited | Professional & scientific | 2015 |
| Emsea Limited | Manufacturing | 2004 |
| Enchant Film Ltd | Information & communication | 2025 |
| Endeavour Performace Nutrition & Sports Therapy Ltd | Health & social care | 2025 |
| Endrell Ltd | Professional & scientific | 2003 |
| Enduratec Ltd | Manufacturing | 2016 |
| Engagesure Ltd | Information & communication | 2026 |
| Engcon (UK) Limited | Wholesale & retail | 2003 |
| ENGINEERING25 Ltd | Professional & scientific | 2021 |
| Enhance Medical Centre Ltd | Health & social care | 2003 |
| ENOSICILIA77 Ltd | Hospitality | 2026 |
| Enso Estates Limited | Construction | 2026 |
| Enso Investments Limited | Financial services | 2026 |
| Ensō Construction Ltd | Construction | 2025 |
| Enterprise Consulting Services Limited | Information & communication | 2023 |
| Environmental Development Technology Limited | Professional & scientific | 1995 |
| Envision Online Ltd | Information & communication | 2012 |
| Epiphany Counselling Ltd | Health & social care | 2022 |
| Epulo Limited | Hospitality | 2005 |
| Equi-Guide Learning CIC | Education | 2025 |
| Equinox Computer Maintenance Limited | Information & communication | 1988 |
| Equinox Group Limited | Professional & scientific | 2001 |
| Equinox Maintenance Limited | Manufacturing | 1989 |
| Eskizara Ltd | Wholesale & retail | 2016 |
| Essential Therapies (Tewkesbury) Limited | Other services | 2012 |
| Eston Property Management Limited | Real estate | 2026 |
| Etaxpol Ltd | Professional & scientific | 2017 |
| Etech Repair Limited | Information & communication | 2022 |
| Ethical Workwear Limited | Wholesale & retail | 2011 |
| Ethicus Consultancy Ltd | Information & communication | 2019 |
| Euro Asian Partnership Limited | Wholesale & retail | 2019 |
| Eurogearshift Limited | Transport & logistics | 2015 |
| Evenflo Water Ltd | Manufacturing | 2025 |
| Eventstorm Limited | Professional & scientific | 2011 |
| Everett Publishing Limited | Arts & recreation | 2026 |
| Everflowai Limited | Administrative & support | 2025 |
| Everything Tall Ltd | Wholesale & retail | 2026 |
| Evesham Live Ltd | Arts & recreation | 2020 |