| Cotswold Group Services Ltd | Administrative & support | 2024 |
| Cotswold Home Improvement (Products) Ltd | Wholesale & retail | 2011 |
| Cotswold Home Improvement (Systems) LLP | | 2011 |
| Cotswold Home Makeovers Limited | Construction | 2018 |
| Cotswold Hot TUB Hire Ltd | Administrative & support | 2026 |
| Cotswold HUE Limited | Wholesale & retail | 2024 |
| Cotswold Oak Products Ltd | Manufacturing | 2025 |
| Cotswold OIL and Heating Ltd | Other services | 2016 |
| Cotswold Plumbing & Heating Supplies Ltd | Wholesale & retail | 2024 |
| Cotswold Premier K9 Ltd | Administrative & support | 2025 |
| Cotswold Properties Ltd | Real estate | 2019 |
| Cotswold Property Developments Limited | Real estate | 2012 |
| Cotswold Serviced Homes Ltd | Real estate | 2022 |
| Cotswold Services Removals Limited | Transport & logistics | 2023 |
| Cotswold Spring Water Limited | Professional & scientific | 1941 |
| Cotswold Talent Partners Limited | Administrative & support | 2023 |
| Cotswold Trades and Labour Ltd | Administrative & support | 2021 |
| Cotteswold Dairy Limited | Manufacturing | 1948 |
| Coultndt Ltd | Construction | 2025 |
| Country Pumpkins Ltd | Health & social care | 2022 |
| County Boiler Services Limited | Construction | 2003 |
| Court Reclamation and Salvage Limited | Administrative & support | 2008 |
| Coversure Bristol Limited | Financial services | 2008 |
| CPS Cheltenham Ltd | Construction | 2020 |
| CPS Developments Ltd | Construction | 2020 |
| Craig Paul Design Ltd | Professional & scientific | 2010 |
| Creative Intuitive Resource Limited | Wholesale & retail | 2018 |
| Creative Vues Ltd | Professional & scientific | 2018 |
| Croome Consulting Ltd | Health & social care | 2016 |
| Cross House Implant Clinic Ltd | Health & social care | 2023 |
| Crossfit Tewkesbury Ltd | Other services | 2024 |
| CRP 1998 Limited | Extraterritorial | 1998 |
| CRP Holdings Limited | Extraterritorial | 2000 |
| CT Dynamic Engineering Ltd | Professional & scientific | 2019 |
| Culinaria Ltd | Hospitality | 2016 |
| Cull Engineering Ltd | Professional & scientific | 2025 |
| Cyber Consultancy Service Ltd | Information & communication | 2025 |
| Cyprianaegisfreights Ltd | Transport & logistics | 2026 |
| D & R Bull Limited | Real estate | 1978 |
| D and D Properties (Glos) Ltd | Households | 2026 |
| D J Nicholls (Plumbing & Heating) Limited | Manufacturing | 2009 |
| D J Perks & Sons Limited | Wholesale & retail | 2017 |
| D P Consultants Ltd | Construction | 2012 |
| D Walker Electrical Ltd | Administrative & support | 2016 |
| D&M Transport (Droitwich) Limited | Transport & logistics | 2018 |
| D'Arcy Wine Merchants Limited | Wholesale & retail | 2006 |
| D.B. Systems Limited | Information & communication | 2018 |
| D.E. Poole Limited | Construction | 2006 |
| DAJ Maintenance Ltd | Construction | 2026 |
| Damian Selfdrive Limited | Transport & logistics | 2011 |
| DAN Baxter Haulage Limited | Transport & logistics | 2025 |
| Dante & Everard Ltd | Information & communication | 2018 |
| Dartex Holdings Limited | Professional & scientific | 2016 |
| David Russo Personal Travel Agent Ltd | Administrative & support | 2022 |
| Davies and Nelson Engineering Company Limited | Administrative & support | 1970 |
| Davis Scientific Treatments Limited | Manufacturing | 2012 |
| Davmay 5 Limited | Professional & scientific | 2001 |
| Davrieeholdings Ltd | Hospitality | 2026 |
| DB Classics Ltd | Wholesale & retail | 2019 |
| Dbpixelhouse Limited | Administrative & support | 1994 |
| DCH Masonry Limited | Administrative & support | 2017 |
| DCH Trans Ltd | Transport & logistics | 2015 |
| DCM Property Management Ltd | Real estate | 2023 |
| DDD Solutions Ltd | Professional & scientific | 2022 |
| Dealworthy Insights Limited | Information & communication | 2026 |
| Dean - Walker Holdings Limited | Health & social care | 2016 |
| Deca Group Limited | Other services | 2024 |
| Deca Non-Ferrous Alloys Ltd | Manufacturing | 2026 |
| Deca Precision Machining Limited | Financial services | 2026 |
| Dedo Catering Ltd | Hospitality | 2024 |
| Delta Vector Engineering Limited | Professional & scientific | 2013 |
| Dentapix Ltd | Information & communication | 2023 |
| Dermont Ltd | Transport & logistics | 2018 |
| Destilled Imagery Ltd | Information & communication | 2024 |
| Developing Ideas Limited | Construction | 2006 |
| Devereux & Hunt Limited | Professional & scientific | 2014 |
| DF Legal LLP | | 2005 |
| DFL Conveyancing Limited | Professional & scientific | 2012 |
| DHB Goldsmiths Limited | Manufacturing | 2006 |
| Diamond & Pearl Lettings Ltd | Real estate | 2022 |
| Digiboox Ltd | Professional & scientific | 2025 |
| Digiguys Ltd | Information & communication | 2021 |
| Digital Conversations Group Limited | Information & communication | 2015 |
| Digital Form Limited | Information & communication | 1996 |
| Digivent Ltd | Professional & scientific | 2018 |
| Dignity Care Services Limited | Health & social care | 2021 |
| Dinghy Limited | Financial services | 2017 |
| Dining @ Ltd | Hospitality | 2019 |
| Dinosaws Ltd | Hospitality | 2026 |
| DJ Stokes & Sons Ltd | Real estate | 2023 |
| DMC Electrical Services Ltd | Construction | 2026 |
| DMH Hydraulics Group Ltd | Manufacturing | 2014 |
| Doggeared Limited | Manufacturing | 2018 |
| Domestic Appliance Distributors Limited | Professional & scientific | 2008 |
| Dorian Builders Limited | Administrative & support | 2014 |
| Dovecote Property Group Limited | Households | 2021 |
| Downend Access Ltd | Real estate | 2026 |
| DOY Ltd | Professional & scientific | 2022 |
| DR Georgina Devenish Ltd | Health & social care | 2023 |
| DR Roxy’S Clinic Limited | Health & social care | 2021 |