| EVOLUTION9 UK Ltd | Wholesale & retail | 2024 |
| Eweniverse Ltd | Wholesale & retail | 2012 |
| Excel First Time Ltd | Education | 2020 |
| Exception PCB Limited | Manufacturing | 1977 |
| Exception PCB Solutions Limited | Manufacturing | 2013 |
| Exceptional Thinking (UK) Ltd | Professional & scientific | 2014 |
| Exeter Renovations Limited | Real estate | 2026 |
| Expert on Energy Ltd | Professional & scientific | 2026 |
| Exstent Limited | Professional & scientific | 2002 |
| F & a Convenience Stores Ltd | Wholesale & retail | 2020 |
| F & K Window Cleaning Services | | 1997 |
| Fadalcnc Limited | Wholesale & retail | 2001 |
| Fadi Kherdaji Limited | Health & social care | 2018 |
| Fairparking Ltd | Transport & logistics | 2019 |
| Fairview Flooring Cheltenham Ltd | Construction | 2020 |
| Faithful Hands Gardening Ltd | Administrative & support | 2024 |
| Fans Fans Fans Ltd | Manufacturing | 2020 |
| Fantastically Fresh Limited | Hospitality | 2018 |
| Farthings Estate Limited | Real estate | 2011 |
| Fassa UK Limited | Administrative & support | 2014 |
| Fastinox Limited | Administrative & support | 2009 |
| Felstar Estates Limited | Real estate | 1996 |
| Fensell Properties Limited | Real estate | 1966 |
| Fensuite Limited | Information & communication | 2024 |
| Fiacre Limited | Health & social care | 2019 |
| Fiddington Manor Limited | Households | 1991 |
| Field & Field Limited | Professional & scientific | 2004 |
| Field Side Transport Limited | Transport & logistics | 2013 |
| Fielding Talent Ltd | Administrative & support | 2026 |
| Fiesta Drinks Ltd | Manufacturing | 2023 |
| Filler Republic Ltd | Other services | 2020 |
| Filtration & Valves Limited | Extraterritorial | 1979 |
| Finance DR Ltd | Administrative & support | 2005 |
| Fine Bespoke Interiors Ltd | Construction | 2019 |
| Finex Interiors Ltd | Construction | 2020 |
| Firmfix Limited | Wholesale & retail | 1991 |
| First Hyperion Ltd | Wholesale & retail | 2025 |
| Fitness Tonic Ltd | Arts & recreation | 2023 |
| Five and Twenties Search Ltd | Professional & scientific | 2025 |
| FKL Property Ltd | Real estate | 2024 |
| Flawless Beauty (SW) Ltd | Other services | 2014 |
| Flawless Hair & Beauty Ltd | Other services | 2014 |
| Flawless Hair Tewkesbury Ltd | Other services | 2014 |
| Fleet Close Park Limited | Hospitality | 2009 |
| Floortex Europe Limited | Wholesale & retail | 2001 |
| Flowmix Ltd | Construction | 2018 |
| FM Group Limited | Construction | 2023 |
| Focus Envelope Solutions Limited | Construction | 2024 |
| Foley Fire Risk Assessment Limited | Administrative & support | 2023 |
| Food IS Art Limited | Hospitality | 2008 |
| Food Linker Limited | Wholesale & retail | 2023 |
| Foodieclub Ltd | Hospitality | 2022 |
| Forestry and Land Limited | Agriculture & fishing | 2022 |
| Forever Fit and Healthy Limited | Extraterritorial | 2025 |
| Forming & Catering Equipment Limited | Wholesale & retail | 2011 |
| Fort Veritas Wealth Ltd | Financial services | 2022 |
| Fosse Fires Limited | Wholesale & retail | 2005 |
| Frazier Properties Limited | Real estate | 1986 |
| Freedom Motor Homes Limited | Construction | 2000 |
| FSC Accountants Ltd | Professional & scientific | 2022 |
| Funw Ltd | Professional & scientific | 2011 |
| Furniture Brands International Limited | Wholesale & retail | 2015 |
| G Gray Farms Limited | Agriculture & fishing | 2003 |
| G K Engineering & Marine Services Limited | Professional & scientific | 2003 |
| G M Hughes Holdings Limited | Construction | 2013 |
| G T Window Products Limited | Construction | 2009 |
| G. Gray Investments (Holdings) Limited | Financial services | 2004 |
| G.A.R. Pollard Engineering (Tewkesbury) Limited | Extraterritorial | 1978 |
| G2K Ltd | Hospitality | 2021 |
| Gabriel Projects Limited | Information & communication | 2026 |
| Gakhar Traders Ltd | Hospitality | 2025 |
| Gall Thomson Environmental Limited | Manufacturing | 1993 |
| Gardiner Brocklehurst Limited | Real estate | 2013 |
| Gardner Audits Ltd | Professional & scientific | 2021 |
| Gaudio Limited | Administrative & support | 2005 |
| Gavin Boniface Building Maintenance Ltd | Construction | 2025 |
| GB Liming Limited | Other services | 2025 |
| Gblp Property Limited | Hospitality | 2026 |
| GC Property DEV. Ltd | Construction | 2025 |
| GC Sports Horses Limited | Agriculture & fishing | 2019 |
| GCB Digital Design Limited | Information & communication | 2012 |
| Geckota Limited | Wholesale & retail | 2012 |
| Gems (Cheltenham) Limited | Manufacturing | 1994 |
| Geodia Limited | Professional & scientific | 2008 |
| Geometric Manufacturing Ltd | Manufacturing | 2002 |
| GER Property & Lettings Ltd | Real estate | 2025 |
| GET Active Physiotherapy and Sports Injury Clinic Ltd | Health & social care | 2014 |
| Getr Holding Ltd | Financial services | 2025 |
| GHG House and Garden Maintenance Ltd | Construction | 2017 |
| Gifts With Care Limited | Extraterritorial | 2018 |
| Gikatitu Ltd | Transport & logistics | 2020 |
| Gilder Agri Limited | Agriculture & fishing | 2020 |
| Gilder Environmental Ltd | Water & waste | 2015 |
| Gilder Hire Limited | Manufacturing | 2015 |
| Gilder Property Limited | Real estate | 2015 |
| Gira Capelli Limited | Other services | 2018 |
| GJS Oakfield Ltd | Real estate | 2025 |
| Glevum Training Services Ltd | Construction | 2024 |
| Gloucestershire Electrical Services Ltd | Construction | 2018 |
| Gloucestershire Surveyors Ltd | Professional & scientific | 2018 |