| Stroud Windows (Gloucestershire) Limited | Construction | 2004 |
| Stuttards Ltd | Wholesale & retail | 2024 |
| SUE Bailey Consulting Ltd | Professional & scientific | 2014 |
| Summit GEO Ltd | Information & communication | 2019 |
| Sunnecotte Property Ltd | Real estate | 2025 |
| Sunny Apple Wood Property Solutions Limited | Households | 2025 |
| Superconscious Living International Limited | Health & social care | 2017 |
| Supply Chain Reaction Ltd | Professional & scientific | 2024 |
| Suzan N Limited | Wholesale & retail | 2014 |
| SW Contracting (Glos) Ltd | Construction | 2012 |
| Swift Fasteners Limited | Wholesale & retail | 1982 |
| T Clarke (Dairy) Limited | Wholesale & retail | 2003 |
| T L Chopra Limited | Health & social care | 2022 |
| T Mason Electrical Ltd | Construction | 2024 |
| T Pillinger Limited | Construction | 2006 |
| T R Lane Performance Fabrications Limited | Manufacturing | 2012 |
| T.C.E. 600 Limited | Wholesale & retail | 2014 |
| TAG Heating Services Ltd | Construction | 2020 |
| TAG Renewables Ltd | Construction | 2025 |
| TAJ & Tara Limited | Hospitality | 2023 |
| Take Two Media Limited | Information & communication | 2024 |
| Tanhill Wind Farm Limited | Energy | 2012 |
| Tapebox Ltd | Wholesale & retail | 2024 |
| Tara Breen Architects Ltd | Administrative & support | 2007 |
| Target 10 Ltd | Professional & scientific | 2023 |
| Taybroh Alloy & Stainless Steels Limited | Wholesale & retail | 1978 |
| Teal Cobra Limited | Hospitality | 2025 |
| Tech-Ties Ltd | Administrative & support | 2023 |
| Techstream Limited | Wholesale & retail | 2012 |
| Tedhealth Limited | Health & social care | 2025 |
| Terrington Management LLP | | 2006 |
| TGF Equipment Ltd | Administrative & support | 2023 |
| THE Accessible Travel & Leisure Limited | Administrative & support | 2000 |
| THE Alteration Co Ltd. | Other services | 2017 |
| THE Arboricultural Association | Other services | 2000 |
| THE Aston Martin Parts Specialists Limited | Wholesale & retail | 2014 |
| THE Auto and Motorcycle Centre Ltd | Wholesale & retail | 2024 |
| THE Autoshop Accident Repair Centre Limited | Wholesale & retail | 2005 |
| THE Beer Depot Ltd | Wholesale & retail | 2023 |
| THE Big Bike Company Limited | Administrative & support | 2009 |
| THE Big DOT Company GL Limited | Information & communication | 2018 |
| THE Billowing Loaf Ltd | Manufacturing | 2017 |
| THE British Croatian Society | Education | 1997 |
| THE Carducci Music Trust | Arts & recreation | 2009 |
| THE Care Marketing Consultancy Ltd | Professional & scientific | 2007 |
| THE Cotswold Curtain Fitter Ltd | Construction | 2022 |
| THE Eesi Employee Ownership Trust Company Limited | Financial services | 2026 |
| THE Ethical Street Food Company Limited | Hospitality | 2011 |
| THE Eyewear Company (London) Ltd | Wholesale & retail | 2007 |
| THE Fallow Way Ltd | Other services | 2025 |
| THE Fifth Dimension Community Leisure Limited | Arts & recreation | 2011 |
| THE Garden Bathroom Company Ltd | Manufacturing | 2020 |
| THE Gatehouse Project Ltd | Administrative & support | 2026 |
| THE Globe (Stonehouse) Ltd | Hospitality | 2026 |
| THE Globe Inn (Stonehouse) Ltd | Hospitality | 2025 |
| THE Heat Hero Ltd | Construction | 2025 |
| THE Horny Revolution Limited | Wholesale & retail | 2020 |
| THE Horse Marling Management Company Limited | Real estate | 2016 |
| THE Lansdown Clinic (Stroud) Limited | Health & social care | 2014 |
| THE Last Hurrah Theatre Company Ltd | Arts & recreation | 2019 |
| THE Luxury Student Ltd | Wholesale & retail | 2016 |
| THE Makerss Wool Crafts Limited | Wholesale & retail | 2025 |
| THE Maltings (Stroud) Management Company Limited | Households | 2018 |
| THE Motor House (Stroud) Limited | Wholesale & retail | 1964 |
| THE Nursing Agency Ltd | Administrative & support | 2022 |
| THE Nympsfield Mushroom Company Ltd | Agriculture & fishing | 2021 |
| THE Oak Frame Carpentry Co. Ltd | Construction | 1999 |
| THE Old Butchers Shop Ltd | Professional & scientific | 2009 |
| THE PE Clothing Company Ltd. | Wholesale & retail | 2017 |
| THE PE Equipment Company Ltd | Wholesale & retail | 2018 |
| THE Physical Education PAL Limited | Information & communication | 2018 |
| THE Plastics and Engineering Co Limited | Manufacturing | 2000 |
| THE Prime Practice Ltd | Health & social care | 2019 |
| THE Queen Stage Limited | Hospitality | 2021 |
| THE Research Box Limited | Professional & scientific | 1999 |
| THE Tipputs Inn Limited | Hospitality | 2017 |
| THE Tree of Life Centre Limited | Health & social care | 2020 |
| THE Whiteshill Star Inn Limited | Construction | 2009 |
| Thebikeshack Ltd | Wholesale & retail | 2025 |
| Themetalhouse Ltd | Wholesale & retail | 2016 |
| Thermal Rooms Limited | Construction | 2022 |
| Thompson Wiring Services Ltd | Manufacturing | 2019 |
| Thorney Close Farm Battery Storage Limited | Energy | 2023 |
| Tiger Construction Equipment Limited | Extraterritorial | 2005 |
| Times and Chimes Ltd | Manufacturing | 2013 |
| Tinkley TIP Restoration Limited | Professional & scientific | 2005 |
| Tiny House Media Ltd | Information & communication | 2019 |
| TIP Top Glasses Limited | Arts & recreation | 2023 |
| TOM Clarkson and Associates Limited | Arts & recreation | 2009 |
| TOM Dalley Financial Solutions Ltd | Financial services | 2019 |
| TOM Ridgway Limited | Professional & scientific | 2022 |
| TOM Vosper Plumbing & Heating Limited | Construction | 2005 |
| Tomachoice Limited | Professional & scientific | 1987 |
| Tony Ryall Training Ltd | Education | 2012 |
| Top Assured Health Care Limited | Health & social care | 2022 |
| Torc Interiors Limited | Professional & scientific | 2017 |
| Town Cars Filling Station Limited | Extraterritorial | 1982 |
| Trackaswim Limited | Information & communication | 2023 |
| Trafalgar - the People Business (UK) Limited | Administrative & support | 2003 |
| Tramatch Ltd | Information & communication | 2024 |