| Senius Care Ltd | Information & communication | 2021 |
| Shades & Blinds SW Ltd | Construction | 2025 |
| Shapes Music Ltd | Information & communication | 2023 |
| Share and Repair Stonehouse CIC | Administrative & support | 2019 |
| Sharetree Limited | Manufacturing | 2004 |
| Shaylors Garage Ltd | Wholesale & retail | 2015 |
| Shch Property Limited | Real estate | 2025 |
| She's Electric Live Ltd | Professional & scientific | 2023 |
| Shield Brickwork & Landscaping Ltd | Construction | 2024 |
| Shropshire ENT Consultants Limited | Health & social care | 2022 |
| Signature Bespoke Carpentry Ltd | Manufacturing | 2025 |
| Simon Lewis Camera Services Ltd | Information & communication | 2019 |
| Simpson Costea Limited | Professional & scientific | 2015 |
| Singleton Physiotherapy Limited | Health & social care | 2016 |
| SIW Construction Management Ltd | Construction | 2025 |
| Sixeyed Consulting Limited | Information & communication | 2009 |
| SJ Supported Living Ltd | Hospitality | 2016 |
| SJB Utilities Consultant Ltd | Information & communication | 2017 |
| SJW Storage Ltd | Transport & logistics | 2020 |
| Skillen Trading Ltd | Wholesale & retail | 2025 |
| Skinny Tree Limited | Administrative & support | 2021 |
| Skinsochic Laser Clinic Limited | Other services | 2022 |
| Skinsochic Ltd | Other services | 2025 |
| Skinsochic Ross Ltd | Other services | 2025 |
| Skinsochic Skinhealth & Aesthetics Limited | Other services | 2022 |
| Skyward Estates Limited | Construction | 2024 |
| Slippy Media Ltd | Administrative & support | 2016 |
| SMA Gloucester Ltd | Construction | 2025 |
| SMA Group Holdings Limited | Financial services | 2023 |
| SMA Property Development Limited | Real estate | 2023 |
| Smartside Innovations Ltd | Wholesale & retail | 2026 |
| Smith & Bradley Renovations Ltd | Construction | 2025 |
| Smith & Buckley Limited | Extraterritorial | 2014 |
| Smiths Carpentry Services Ltd | Construction | 2022 |
| Smolinski's Commercial Cleaning Limited | Administrative & support | 2004 |
| Smoothline Engineering Services Limited | Wholesale & retail | 2000 |
| SMP Sheet Metal Ltd | Manufacturing | 2007 |
| Smuj Group Limited | Manufacturing | 2026 |
| Solar Carbon Exchange Ltd | Extraterritorial | 2025 |
| Solar Nation UK Limited | Wholesale & retail | 2016 |
| Solara Exled Projects Ltd | Wholesale & retail | 2010 |
| Solarapt Limited | Wholesale & retail | 2023 |
| Sound Educational Audio Learning Limited | Education | 1986 |
| South Farm Solar Ltd. | Energy | 2018 |
| Sovereign Property & Electrical Ltd | Construction | 2012 |
| Spa Cottages Management Ltd | Households | 2015 |
| Space AM Limited | Professional & scientific | 2021 |
| Spar Construction Limited | | 1986 |
| Speak Easy Consultancy Ltd | Professional & scientific | 2025 |
| Specialised Procurements International (UK) Limited | Wholesale & retail | 1994 |
| Spenveal Properties Ltd | Real estate | 2025 |
| Spirit and Wild Ltd | Professional & scientific | 2018 |
| Splash Swimming Academy Ltd | Education | 2018 |
| Spring Farm (Frocester) LLP | | 2014 |
| Springfield Business Centre Limited | Households | 1988 |
| Springfield PV Ltd | Energy | 2024 |
| Sprocket Young Ltd | Professional & scientific | 2011 |
| SSM UK Services Ltd | Professional & scientific | 2025 |
| ST Annes Garden Liverpool Limited | Real estate | 2021 |
| ST Peter's Close (Rodmarton) Management Company Limited | Water & waste | 1955 |
| Stackingpixels Ltd | Information & communication | 2025 |
| Staines Enterprises Ltd | Real estate | 2016 |
| Staitech Limited | Manufacturing | 1997 |
| Standish Court Arch Limited | Households | 2002 |
| Stanford Contemporary Arts Limited | Arts & recreation | 2005 |
| Star Green Heating Limited | Construction | 2017 |
| Star Office Services Ltd | Administrative & support | 2025 |
| Starterp Ltd | Information & communication | 2020 |
| Steanbridge Consulting Limited | Professional & scientific | 2024 |
| Steel Camel UK Limited | Manufacturing | 2018 |
| Steele Associates Ltd | Professional & scientific | 2025 |
| Steve Merrett Limited | Construction | 2015 |
| Steven Price Design Limited | Professional & scientific | 2014 |
| Stevo Recovery Ltd | Transport & logistics | 2022 |
| Stonecat Collective Ltd | Information & communication | 2018 |
| Stonehouse (NO.2) Management Company Limited | Households | 1992 |
| Stonehouse Autoparts Limited | Wholesale & retail | 2003 |
| Stonehouse Convenience Ltd | Wholesale & retail | 2016 |
| Stonehouse Fabrication Ltd | Manufacturing | 2025 |
| Stonehouse Furniture Design Limited | Manufacturing | 2014 |
| Stonehouse Hand Car Wash Ltd | Wholesale & retail | 2019 |
| Stonehouse Motor Company (SW) Ltd | Wholesale & retail | 2015 |
| Stonehouse Rentals Limited | | 1983 |
| Stonehouse Veterinary Services Ltd | Professional & scientific | 2021 |
| Storage Systems (Glos) Limited | Construction | 1997 |
| Strandz Hair Salon Limited | Other services | 2020 |
| Streamline Dispense Limited | Manufacturing | 2000 |
| Strichen North Battery Storage Limited | Administrative & support | 2018 |
| Strichen South Battery Storage Limited | Administrative & support | 2018 |
| Stroud Alarms Fire & Security Limited | Administrative & support | 2007 |
| Stroud Alarms Limited | Extraterritorial | 2013 |
| Stroud Bookshops Limited | Wholesale & retail | 2002 |
| Stroud Care Services Limited | Other services | 1998 |
| Stroud CC Trading Ltd | Hospitality | 2024 |
| Stroud Electrical Services UK Ltd | Construction | 2009 |
| Stroud Engineering Services Limited | Professional & scientific | 2019 |
| Stroud Grounds Maintenance Ltd | Administrative & support | 2021 |
| Stroud Landscapes Ltd | Administrative & support | 2020 |
| Stroud Metal Company Limited | Manufacturing | 1926 |
| Stroud Plastics Company Limited | Manufacturing | 2002 |