| RA Takeaway Limited | Hospitality | 2021 |
| Raffles Forest School Ltd | Education | 2014 |
| Raffles Media Ltd | Information & communication | 2022 |
| Raglan Associates and Company Limited | Professional & scientific | 1999 |
| Ranchers Steakhouse Ltd | Hospitality | 2018 |
| Ransome Memorials Limited | Other services | 2010 |
| Rapide Limited | Administrative & support | 2005 |
| RAW Edge Leather Ltd | Manufacturing | 2021 |
| Razor Barber Gloucester Ltd | Other services | 2026 |
| Rbgb UK Ltd | Real estate | 2025 |
| Reap Consulting Ltd | Professional & scientific | 2018 |
| Rebel Consultancy Limited | Education | 2021 |
| Reconnect Gloucestershire CIC | Other services | 2022 |
| Red Kite Environment Ltd. | Professional & scientific | 2002 |
| Red Lion (Kings Stanley) Management Ltd | Households | 1996 |
| Red Tiger Properties Limited | Real estate | 2023 |
| Redcello Limited | Information & communication | 2013 |
| Redler Limited | Manufacturing | 2023 |
| Redswebs & Shop Design Ltd | Information & communication | 2018 |
| Reflex Adventure Products Ltd | Manufacturing | 2023 |
| Relay Industries Limited | Professional & scientific | 2015 |
| Remmias Limited | Professional & scientific | 2024 |
| Renbat 1 Limited | Energy | 2022 |
| Renbat 13 Limited | Energy | 2023 |
| Renbat 14 Limited | Energy | 2023 |
| Renbat 15 Limited | Energy | 2023 |
| Renbat 6 Limited | Energy | 2022 |
| Renbat Developments Limited | Energy | 2024 |
| Rencore Limited | Financial services | 2022 |
| Renosash Limited | Construction | 2022 |
| Resilia Limited | Professional & scientific | 2024 |
| Resilient Future Fund Limited | Financial services | 2022 |
| Retro Track and Air (UK) Limited | Manufacturing | 2000 |
| Revek Ltd | Other services | 2007 |
| RF Partners Ltd | Financial services | 2013 |
| Rheos 1 Ltd | Energy | 2024 |
| Rich Improvements Ltd | Construction | 2022 |
| Richard Faith Architecture Bureau Ltd | Professional & scientific | 2017 |
| Richard James Upholstery Limited | Wholesale & retail | 2016 |
| Richmond Construction Services Ltd | Construction | 2023 |
| Ridge & Furrow Ltd | Administrative & support | 2011 |
| Rightsource C.I.C. | Information & communication | 2023 |
| Risaltren Limited | Administrative & support | 2026 |
| Rivers Recruitment Limited | Administrative & support | 2021 |
| Riverswan Limited | Professional & scientific | 2017 |
| RJC & Associates Limited | Professional & scientific | 2012 |
| RJM Repair & Paint Ltd | Wholesale & retail | 2023 |
| RLF Consultants Limited | Professional & scientific | 2025 |
| Robert Hancock Building and Design Limited | Construction | 2011 |
| Robinson Associates ( Stroud ) Ltd | Extraterritorial | 2003 |
| Robyn Morris Counselling & Psychotherapy Ltd | Health & social care | 2023 |
| Romney Finance Limited | Financial services | 2018 |
| Romney Surbiton Holdings Limited | Financial services | 2018 |
| Romney Surbiton Limited | Financial services | 2018 |
| Romney Tetbury Holdings Limited | Financial services | 2018 |
| Romney Tetbury Limited | Financial services | 2018 |
| Rooksmoor Mills Limited | Real estate | 2005 |
| Rope Work Solutions Ltd. | Manufacturing | 2014 |
| Rosalinda KITCHEN10 Ltd | Hospitality | 2023 |
| Rowan Food & Biomass Engineering Limited | Hospitality | 2008 |
| Rowe Equine Limited | Professional & scientific | 2011 |
| RS Autotechnik Limited | Wholesale & retail | 2020 |
| RS Control and Automation Limited | | 2011 |
| RSP Vehicle Services Ltd | Wholesale & retail | 2024 |
| Rune Interiors Ltd | Construction | 2024 |
| RW Wealth Partners Limited | Financial services | 2026 |
| Rydercar Limited | Wholesale & retail | 2003 |
| Ryeford Engineering (Glos) Limited | Manufacturing | 2003 |
| Ryeford Estate Road Ltd | Real estate | 2022 |
| Ryeford Specialist Services Limited | Administrative & support | 2003 |
| S Hobbs Ltd | Administrative & support | 2015 |
| S P J Atkinson Limited | Wholesale & retail | 2017 |
| S W Aviation Limited | Manufacturing | 2017 |
| S.S.C. Project Management Services Ltd | Professional & scientific | 2017 |
| Sainty Property Ltd | Real estate | 2023 |
| Salcombe Boats and Boards Ltd | Transport & logistics | 2020 |
| Samexpert Ltd | Information & communication | 2024 |
| Samsak Technologies Ltd | Information & communication | 2022 |
| Sandpiper Consultancy Services Ltd | Information & communication | 2012 |
| Sarah Burghard Designs Limited | Professional & scientific | 2014 |
| Sarah DG Design Limited | Wholesale & retail | 2023 |
| Sarin Healthcare Limited | Real estate | 2017 |
| Sartorius Stedim LAB Limited | Manufacturing | 1992 |
| Satis Electronics Ltd | Manufacturing | 2024 |
| Saunter Dogs Ltd | Other services | 2025 |
| Sbkacs Limited | Professional & scientific | 2017 |
| Sbsa Limited | Education | 2019 |
| SC Damp Surveying Ltd | Construction | 2025 |
| Schreiber Foods UK Limited | Manufacturing | 2021 |
| Scott Aerospace Holdings Limited | Financial services | 2006 |
| Scott Aerospace Limited | Professional & scientific | 1994 |
| Scott Hanson Limited | Hospitality | 2015 |
| Scott Property Investments Ltd | Real estate | 2025 |
| Scott Total Security Limited | Wholesale & retail | 2004 |
| Scsc Rentals Ltd | Real estate | 2022 |
| SDM Insight Limited | Administrative & support | 2006 |
| SDN Sport Consulting Ltd | Education | 2025 |
| Search and Recruit Limited | Administrative & support | 2025 |
| Securadata Limited | Information & communication | 2016 |
| Selicor Consulting Ltd | Professional & scientific | 2022 |