| Treble S Limited | Professional & scientific | 1999 |
| Tredington Ltd | Financial services | 2024 |
| TRI Polus Ltd. | Professional & scientific | 2002 |
| Tricel (Gloucester) Limited | Manufacturing | 1993 |
| Tricel Holdings (UK) Limited | Professional & scientific | 1999 |
| Tricia Zooms Limited | Education | 2021 |
| TRP Media Limited | Other services | 2022 |
| Truhouse Franchising Ltd | Professional & scientific | 2021 |
| Truhouse. (Stroud) Ltd | Construction | 2021 |
| Tulabell Limited | Health & social care | 2020 |
| Turnkey Construction Developments Ltd | Administrative & support | 2011 |
| Turtle Productions Ltd | Administrative & support | 2004 |
| Twocan Inclusive Theatre Company Limited | Arts & recreation | 2014 |
| Ty-Isaf Innovation Limited | Professional & scientific | 2025 |
| UK Dietetics Ltd | Health & social care | 2024 |
| Ultimate Building Plastics Ltd | Professional & scientific | 2002 |
| Ultracat Systems Limited | Wholesale & retail | 2010 |
| Union Brands Ltd | Manufacturing | 2011 |
| Union DAC Ltd | Manufacturing | 2024 |
| Unique Groundworks Limited | Construction | 2019 |
| Uniquemix Limited | Wholesale & retail | 1997 |
| Upfield House Management Co. Ltd. | Households | 2014 |
| Valentine Wigs Limited | Wholesale & retail | 2016 |
| Valora 68 Properties Ltd | Real estate | 2025 |
| Van Food 22 Limited | Hospitality | 2024 |
| Veritan Consultancy Limited | Professional & scientific | 1998 |
| Veritas Consulting Group Limited | Professional & scientific | 2026 |
| Vhpartners Ltd | Hospitality | 2024 |
| Victoria Road Flat Management Company Limited | Households | 2012 |
| View Recruitment Limited | Administrative & support | 2012 |
| Vigilanter Ltd | Professional & scientific | 2022 |
| Vinycomb Bate Ltd | Manufacturing | 2007 |
| Virginia Plumbing Enterprise Ltd | Construction | 2018 |
| Vixus Property Advisory Ltd. | Professional & scientific | 2019 |
| Vixus Property Group Ltd. | Professional & scientific | 2017 |
| VJ Construction Ltd | Construction | 2015 |
| VLT O&S UK Ltd | Construction | 2025 |
| Voltalia UK Ltd | Construction | 2011 |
| VUK Investment 1 Limited | Financial services | 2023 |
| Vulcain Engineering Ltd | Professional & scientific | 2006 |
| Vulcain Engineering UK Holdings Limited | Professional & scientific | 2021 |
| W Risby Commercial Limited | Construction | 2012 |
| W Risby Group Limited | Professional & scientific | 2012 |
| W Risby Residential Limited | Construction | 2012 |
| W. Risby Limited | Construction | 1972 |
| W4PRO Limited | Information & communication | 2020 |
| Walkonomy Limited | Information & communication | 2019 |
| Wallbridge Storage Limited | Real estate | 2019 |
| Walnut Tree Data Ltd | Information & communication | 2019 |
| Ward & Co. (Building Conservation) Limited | Construction | 2000 |
| Wave Electrical (Stroud) Ltd | Construction | 2025 |
| WE ARE Hedgy Limited | Wholesale & retail | 2022 |
| Weald Solar Limited | Energy | 2014 |
| Webber Motor Group Ltd | Wholesale & retail | 2023 |
| Wellington Property Management Ltd | Households | 2022 |
| Wellspect Limited | Wholesale & retail | 2019 |
| Wessex Integrated Safety Health Environmental Services Limited | Professional & scientific | 2001 |
| West Solutions Kent Ltd | Construction | 2024 |
| Westend Office Suites Limited | Real estate | 2014 |
| Wharfdale Management Ltd | Households | 2017 |
| Whippys Wheels Ltd | Wholesale & retail | 2026 |
| White Villa Films Limited | Information & communication | 2007 |
| Whiteminhill Solar Limited | Energy | 2021 |
| Wild 4X4 Ltd | Manufacturing | 2017 |
| Wild Cat Technologies Ltd | Information & communication | 2019 |
| William Hamilton & Sons (Contractors) Limited | Transport & logistics | 1990 |
| Wilsley House Management Company Limited | Households | 2020 |
| Windward Marine Limited | Wholesale & retail | 1971 |
| Winfield & Ponting Direct Ltd | Wholesale & retail | 2023 |
| Wise Life Ltd | Manufacturing | 2020 |
| Wisloe Farm Services Limited | Agriculture & fishing | 2014 |
| WJT Consultancy Services Limited | Manufacturing | 2009 |
| Woodchester M O T Centre Limited | Wholesale & retail | 2009 |
| Woodchester Mansion Trading Limited | Administrative & support | 2016 |
| Woodchester Service Station Limited | Extraterritorial | 1976 |
| Woodland Stalking UK Ltd | Agriculture & fishing | 2015 |
| Woodmancote Garage (Services) Limited | Professional & scientific | 1975 |
| Woodpark Limited | Arts & recreation | 1992 |
| Woodpecker Creation Ltd | Information & communication | 2020 |
| Woodward Developments (Dursley) Limited | Construction | 1996 |
| Work Smarter Advisory Limited | Administrative & support | 2024 |
| World Guitars Limited | Wholesale & retail | 2016 |
| Worship & Wisdom Ltd | Information & communication | 2025 |
| Wpsoftware Ltd | Information & communication | 2016 |
| Write Wills for YOU Limited | Financial services | 2016 |
| Wycliffe College (Incorporated) | Education | 1931 |
| X-Tech Medical Limited | Manufacturing | 2011 |
| Xograph Healthcare Limited | Manufacturing | 1967 |
| Y & L Company Limited | Wholesale & retail | 1999 |
| Y I P Estates Ltd | Real estate | 2024 |
| Yogafields Ltd. | Professional & scientific | 2014 |
| Yoodee Ltd | Information & communication | 2021 |
| YP Property Holdings Ltd | Real estate | 2025 |
| Zaika Stroud Ltd | Hospitality | 2019 |
| ZEN Protect Limited | Administrative & support | 2024 |
| ZJF Ltd | Health & social care | 2021 |
| Zuanshi Limited | Health & social care | 2021 |