| Stoke Mart Limited | Wholesale & retail | 2024 |
| Stoke Mechanical Services Limited | Professional & scientific | 2017 |
| Stoke Motor Breakers Ltd | Wholesale & retail | 2019 |
| Stoke Motor Sales Ltd | Wholesale & retail | 2016 |
| Stoke Motorz Ltd | Wholesale & retail | 2024 |
| Stoke Nails Ltd | Other services | 2026 |
| Stoke on Clay CIC | Arts & recreation | 2023 |
| Stoke on Trent College | Education | 2012 |
| Stoke on Trent Electrical Services Ltd | Construction | 2021 |
| Stoke on Trent Pottery Limited | Manufacturing | 2025 |
| Stoke on Trent Roofing Limited | Construction | 2025 |
| Stoke on Trent Rubbish Removal Ltd | Water & waste | 2025 |
| Stoke Orthodontic Services Limited | Professional & scientific | 2009 |
| Stoke Pipe Installations Ltd. | Construction | 2012 |
| Stoke Properties 2 Ltd | Construction | 2026 |
| Stoke Property Group Ltd | Real estate | 2024 |
| Stoke Real Estate Limited | Real estate | 2021 |
| Stoke Recycling Ltd | Water & waste | 2024 |
| Stoke Shop Ltd | Wholesale & retail | 2025 |
| Stoke Skin Ltd | Other services | 2026 |
| Stoke Smart Convenience Ltd | Wholesale & retail | 2025 |
| Stoke Solicitors Limited | Professional & scientific | 2021 |
| Stoke Solid Fuels Ltd | Agriculture & fishing | 2025 |
| Stoke Stone Stafford Private Hire Ltd | Transport & logistics | 2025 |
| Stoke Studios Ltd | Professional & scientific | 2017 |
| Stoke Taxi Consultants Ltd | Professional & scientific | 2025 |
| Stoke Traders Ltd | Wholesale & retail | 2023 |
| Stoke Trampoline Gymnastics Club Ltd | Arts & recreation | 2026 |
| Stoke Transport Services Ltd | Transport & logistics | 2022 |
| Stoke Travel Ltd | Transport & logistics | 2020 |
| Stoke Tyres Ltd | Wholesale & retail | 2023 |
| Stoke UK Property Ltd | Real estate | 2026 |
| Stoke Van Hire (SVH) Limited | Administrative & support | 2024 |
| Stoke Wholesale Ltd | Wholesale & retail | 2024 |
| Stoke Your Gratitude C.I.C. | Health & social care | 2017 |
| Stoke-L-Investments (UK) Ltd | Other services | 2025 |
| Stoke-On-Trent Boatbuilding Company Limited | Manufacturing | 1979 |
| Stoke-On-Trent Community Partnership Railway Enterprise Centre Limited | Real estate | 1990 |
| Stoke-On-Trent Foodbank | Health & social care | 2012 |
| Stoke-On-Trent Modern Glass Co.Limited | Construction | 1965 |
| Stokebazaar Ltd | Wholesale & retail | 2025 |
| Stokebinservices Limited | Water & waste | 2026 |
| Stoked UP Food Ltd | Hospitality | 2024 |
| Stoked Ventures Limited | Information & communication | 2017 |
| Stokemt Ltd | Education | 2024 |
| Stokepol Construction Ltd | Construction | 2022 |
| Stokes Office Furniture Ltd | Wholesale & retail | 2022 |
| Stokes Quality Cleaning Limited | Administrative & support | 2026 |
| Stoketrade Ltd | Wholesale & retail | 2023 |
| Stokex Supplies Ltd | Wholesale & retail | 2026 |
| Stolen Glimpse Limited | Wholesale & retail | 2015 |
| Stone Aesthetics and Wellbeing Ltd | Health & social care | 2023 |
| Stone and Stone (Consultants) Ltd | Professional & scientific | 2022 |
| Stone Bespoke Narrowboats Limited | Manufacturing | 2022 |
| Stone Flames Takeaway Limited | Hospitality | 2018 |
| Stone Road Commercial Finance Limited | Financial services | 2010 |
| Stone Road Investments Limited | Financial services | 2024 |
| Stone-For-Less Ltd | Wholesale & retail | 2015 |
| Stonebridge Builders Merchants Ltd | Wholesale & retail | 2024 |
| Stonebridge Motor Works Ltd | Wholesale & retail | 2026 |
| Stonehenge Consulting Ltd | Professional & scientific | 2020 |
| Stonescale Craftsmen Limited | Manufacturing | 2025 |
| Stoney Gate Management Company Limited | Households | 2019 |
| Stoney Rock Lettings Limited | Hospitality | 2004 |
| Storatainer Ltd | Arts & recreation | 2026 |
| Store IT ALL Stoke Ltd | Other services | 2024 |
| Storm Engineering Ltd | Professional & scientific | 2025 |
| Storm Home Improvements UK Ltd | Construction | 2009 |
| Storzone Limited | Wholesale & retail | 2025 |
| STP (Staffordshire) Limited | Financial services | 2014 |
| Strange Ideas Ltd | Information & communication | 2017 |
| Strategic Fintax Consultants Ltd | Professional & scientific | 2020 |
| Strategy 365 Limited | Information & communication | 2016 |
| Stratifae Ltd | Information & communication | 2025 |
| Stratum Financial Services Ltd | Financial services | 2018 |
| Strauss Developments Ltd | Construction | 2024 |
| Streamixworld Limited | Information & communication | 2024 |
| Streamlined Solutions Ltd | Professional & scientific | 2018 |
| Street Services (UK) Limited | Construction | 2007 |
| Street-Hoskins Solutions Limited | Professional & scientific | 2018 |
| Street-Smart Enterprises Ltd | Households | 2024 |
| Streetsmartgamers.Com Ltd | Information & communication | 2020 |
| Streetvan Ltd | Hospitality | 2025 |
| Stretton Denman Limited | Real estate | 2018 |
| Strides Travels Ltd | Transport & logistics | 2023 |
| Stringer & Pickford Ltd | Administrative & support | 2001 |
| Strivaal Limited | Information & communication | 2019 |
| Strmax Ltd | Wholesale & retail | 2020 |
| Strongberg Limited | Manufacturing | 2014 |
| Structural Design Services Limited | Professional & scientific | 1998 |
| Structural Engineer Calcs Ltd | Construction | 2020 |
| Structural Steel Buildings Ltd | Construction | 2019 |
| Structural Surveys Nationwide Ltd | Construction | 2021 |
| STS Gates Ltd | Manufacturing | 2025 |
| STS Solutions Ltd | Construction | 2014 |
| STS Vehicle Movements Limited | Transport & logistics | 2015 |
| Stubbs D Limited | Wholesale & retail | 2022 |
| Stubbs Engineering Ltd | Professional & scientific | 2026 |
| Stubbs RLJ Property Ltd | Real estate | 2025 |
| Stud Universe Limited | Administrative & support | 2023 |