| Station Road Properties Ltd | Real estate | 2018 |
| Stayssi Ltd | Real estate | 2026 |
| STC Screeding Contracting Limited | Construction | 2019 |
| STE F&B Ltd | Hospitality | 2015 |
| Stealth Architecture Ltd | Agriculture & fishing | 2020 |
| Steam Vape Limited | Wholesale & retail | 2023 |
| Steel Wheelz UK Limited | Manufacturing | 2020 |
| Steel&Storage Ltd | Construction | 2024 |
| Steele Public Relations Limited | Administrative & support | 2021 |
| Steelite International Limited | Manufacturing | 1983 |
| Steelwork Services (Midlands) Limited | Manufacturing | 2009 |
| Steeple Tech Ltd | Professional & scientific | 2025 |
| Steer TEC Ltd | Manufacturing | 2020 |
| Stella 4 Property Ltd | Real estate | 2022 |
| Stellarroute Transport and Logistics 2701 Ltd | Transport & logistics | 2025 |
| Steller Blue Limited | Financial services | 2020 |
| Stelloyd Limited | Professional & scientific | 2025 |
| Stenderup Group UK Ltd | Financial services | 2023 |
| Step Forward Children’S Homes Ltd | Health & social care | 2026 |
| Step Inside Investment Properties Limited | Real estate | 2019 |
| Step UP Stoke CIC | Information & communication | 2022 |
| Stephen J Allen Limited | Transport & logistics | 2017 |
| Stephen Latham Fuel Doctor (Stoke) Ltd | Manufacturing | 2010 |
| Stephen Richardson Limited | Construction | 2008 |
| Stepping Stones (Grange) Limited | Other services | 2009 |
| Stepque Travel Limited | Transport & logistics | 2009 |
| Sterling Cash Flow Ltd | Professional & scientific | 2026 |
| Sterling Facility Care Ltd | Administrative & support | 2025 |
| Sterling Mint Ltd | Wholesale & retail | 2020 |
| Sterling Motors Ltd | Wholesale & retail | 2025 |
| Sterlingkart Ltd | Wholesale & retail | 2026 |
| Sterling’S Services Ltd | Real estate | 2020 |
| Sterren People Consulting Ltd | Professional & scientific | 2025 |
| Steve Perry Creative Limited | Professional & scientific | 2013 |
| Steve Sherlock Ltd | Financial services | 2022 |
| Steven Dawson Services Limited | Construction | 2015 |
| Steven Deaville Limited | Information & communication | 2019 |
| Steven Howard Gallery Limited | Wholesale & retail | 2025 |
| Stevens Defence LLP | | 2023 |
| Stevenson Brown Ltd | Administrative & support | 2021 |
| STF Cleaning Ltd | Administrative & support | 2025 |
| STH Transport Management Ltd | Transport & logistics | 2024 |
| Sticker Stocker Ltd | Wholesale & retail | 2011 |
| Sticktec Ltd | Manufacturing | 2022 |
| Stillness 924 Limited | Construction | 2008 |
| Stionne Ltd | Other services | 2020 |
| Stir FRY Limited | Hospitality | 2026 |
| Stirling Outdoors Limited | Wholesale & retail | 2014 |
| Stirling Rigging and Transport Ltd | Transport & logistics | 2016 |
| Stitch and Press Ltd | Professional & scientific | 2021 |
| Stlh Group Ltd | Information & communication | 2025 |
| Stock OFF License Ltd | Wholesale & retail | 2026 |
| Stock Vision Limited | Wholesale & retail | 2025 |
| Stockbridge and Blaze Ltd | Wholesale & retail | 2026 |
| Stockton Brook Time & Space CIC | Education | 2020 |
| Stockwell Property Investments Limited | Construction | 1998 |
| Stoddards Holdings Limited | Financial services | 2026 |
| Stofix Holdings (UK) Ltd | Other services | 2018 |
| Stofix UK Ltd | Construction | 2014 |
| Stoke 365 Limited | Health & social care | 2023 |
| Stoke Accounting Services Ltd | Professional & scientific | 2025 |
| Stoke and Stay Ltd | Hospitality | 2025 |
| Stoke Bespoke Bathrooms Limited | Construction | 2021 |
| Stoke Bodyshop Limited | Wholesale & retail | 2021 |
| Stoke Business Hub Ltd | Manufacturing | 2023 |
| Stoke Car Sales Limited | Wholesale & retail | 2015 |
| Stoke Carpet Centre Limited | Wholesale & retail | 2015 |
| Stoke Carpets and Furniture Limited | Wholesale & retail | 2021 |
| Stoke Chicken Limited | Wholesale & retail | 2013 |
| Stoke City (Property) Limited | Real estate | 1996 |
| Stoke City Football Club Limited | Arts & recreation | 1908 |
| Stoke City Foundation | Arts & recreation | 2003 |
| Stoke City Holdings Limited | Professional & scientific | 2006 |
| Stoke Civils & Utilities Ltd | Construction | 2025 |
| Stoke Clear & Pave Ltd | Water & waste | 2026 |
| Stoke Customs Ltd | Wholesale & retail | 2024 |
| Stoke Dental Limited | Extraterritorial | 2025 |
| Stoke Discount Ltd | Wholesale & retail | 2023 |
| Stoke Drainage Services Ltd | Water & waste | 2022 |
| Stoke Electronic Services Ltd | Manufacturing | 2022 |
| Stoke Estates Ltd | Real estate | 2021 |
| Stoke Express Ltd | Hospitality | 2026 |
| Stoke Express One Ltd | Hospitality | 2025 |
| Stoke Fairmart Ltd | Wholesale & retail | 2025 |
| Stoke Fast Food Limited | Hospitality | 2025 |
| Stoke FIX Ltd | Construction | 2026 |
| Stoke Flooring Solutions Ltd | Construction | 2014 |
| Stoke FOG Ltd | Wholesale & retail | 2025 |
| Stoke Galvanising Limited | Manufacturing | 1988 |
| Stoke GAS and Fireplace Centre Limited | Wholesale & retail | 1998 |
| Stoke Grill Hartshill Limited | Hospitality | 2024 |
| Stoke Heating Solutions Ltd | Construction | 2022 |
| Stoke Housing Property Limited | Real estate | 2025 |
| Stoke Kebab Ltd | Wholesale & retail | 2025 |
| Stoke Kebab Range Limited | Hospitality | 2026 |
| Stoke Laptops Ltd | Wholesale & retail | 2021 |
| Stoke Lara Mini Market Limited | Wholesale & retail | 2025 |
| Stoke Laser & Punch Ltd | Manufacturing | 2010 |
| Stoke Laser Ltd | Manufacturing | 2015 |
| Stoke Market Ltd | Wholesale & retail | 2025 |