| Solinski Ltd | Transport & logistics | 2019 |
| Solsa Ltd | Wholesale & retail | 2018 |
| Somerville Healthcare Limited | Wholesale & retail | 2025 |
| Sometrans Limited | Transport & logistics | 2019 |
| Sonas (North West) Limited | Professional & scientific | 2017 |
| Sood Marketing Limited | Professional & scientific | 2017 |
| Sophia Luisa Ltd | Professional & scientific | 2023 |
| SOS Auto Electrics Ltd | Wholesale & retail | 2023 |
| SOS Homecare Ltd. | Health & social care | 2002 |
| SOS Master Home Ltd | Other services | 2014 |
| Souhil Barber Ltd | Other services | 2026 |
| Sound Empire Ltd | Information & communication | 2013 |
| Source Invest Limited | Real estate | 2019 |
| South West Motor Sales Ltd | Wholesale & retail | 2024 |
| Southgate Property Solutions Ltd | Real estate | 2023 |
| Sowing Seeds Haydock Ltd | Administrative & support | 2024 |
| Sowley Properties Limited | Real estate | 2019 |
| SP Services North West Ltd | Construction | 2025 |
| SP91 Logistics Limited | Transport & logistics | 2020 |
| Space Architectural Limited | Construction | 2019 |
| Spanish Cleaning Haydock Ltd | Administrative & support | 2024 |
| Sparkling House by Laura Ltd. | Wholesale & retail | 2018 |
| Sparta ST Helens Ltd | Hospitality | 2024 |
| Specialised Glass Cutting Limited | Manufacturing | 2004 |
| Specialist Access Engineering Ltd | Manufacturing | 2015 |
| Specsellers Limited | Health & social care | 2006 |
| Spectrum Salon Space Ltd | Real estate | 2025 |
| Speed Daro Transport Limited | Transport & logistics | 2018 |
| Speights Estates Ltd | Real estate | 2026 |
| Spencer Stanhope Ltd | Other services | 2016 |
| SPG Travel Ltd | Administrative & support | 2024 |
| Spinney Training Hub CIC | Education | 2019 |
| Spires 1961 Limited | Extraterritorial | 2015 |
| Splash and Shake Animal Hydrotherapy Limited | Professional & scientific | 2016 |
| Splicer Services Ltd | Professional & scientific | 2017 |
| Sporting Club ST. Helens Limited | Professional & scientific | 1999 |
| Sports Trading Media Ltd | Other services | 2019 |
| SPR North West Ltd | Wholesale & retail | 2023 |
| Spray Tech North West Ltd | Construction | 2021 |
| Spraynovate Ltd | Construction | 2024 |
| Springfield Mobile (Lancs) Limited | Manufacturing | 1976 |
| Spwss Limited | Administrative & support | 2015 |
| Spyne Ltd | Information & communication | 2024 |
| SQL Remedy Ltd | Other services | 2020 |
| Squimble Limited | Transport & logistics | 2025 |
| Squiz Consulting Ltd | Professional & scientific | 2024 |
| SR Property Law Limited | Professional & scientific | 2026 |
| SRK Partners Ltd | Transport & logistics | 2021 |
| SS Driver Services Ltd | Transport & logistics | 2021 |
| SS Fire Ltd | Construction | 2024 |
| SSJ Civils Ltd | Construction | 2019 |
| Ssjj Properties Ltd | Real estate | 2023 |
| SSK Design Solutions Ltd | Professional & scientific | 2024 |
| ST Elian Commercial Limited | Professional & scientific | 2019 |
| ST Farrelly Ltd | Administrative & support | 2021 |
| ST Helens Body Piercing Ltd | Other services | 2018 |
| ST Helens Chippy Ltd | Hospitality | 2026 |
| ST Helens Chiropractic & Acupuncture Clinic Ltd | Health & social care | 2003 |
| ST Helens Community Transport C.I.C. | Transport & logistics | 2014 |
| ST Helens Darts Shop Ltd | Wholesale & retail | 2010 |
| ST Helens Glass Limited | Construction | 2024 |
| ST Helens Hand Car Wash Limited | Other services | 2026 |
| ST Helens Healthcare Limited | Financial services | 2018 |
| ST Helens Law Limited | Professional & scientific | 2005 |
| ST Helens Martial Arts and Fitness Academy Limited | Education | 2022 |
| ST Helens Millennium Centre Services Limited | Wholesale & retail | 2008 |
| ST Helens Motors Ltd | Wholesale & retail | 2021 |
| ST Helens Pizza & Kebab Express Ltd | Hospitality | 2024 |
| ST Helens Roofing Ltd | Construction | 2025 |
| ST Helens Rugby Football Club Limited | Arts & recreation | 1937 |
| ST Helens Stadium Limited | Real estate | 2009 |
| ST Helens Supplies Limited | Wholesale & retail | 2025 |
| ST Helens the Best ME CIC | Education | 2020 |
| ST Helens Third Sector Consortium | Other services | 2015 |
| ST Helens Town Limited | Arts & recreation | 2002 |
| ST Mary's Bay Garage Limited | Wholesale & retail | 2019 |
| ST. Helens Academy of Beauty Ltd | Education | 2020 |
| ST. Helens Dial a Pizza Limited | Wholesale & retail | 2003 |
| ST. Helens Flooring Specialist Limited | Wholesale & retail | 2024 |
| ST. Helens Muay Thai Limited | Arts & recreation | 2022 |
| ST. Helens Rota Limited | Health & social care | 1997 |
| ST. Helens Service & MOT Centre Limited | Wholesale & retail | 2004 |
| St.H Plumbing & Heating Ltd | Construction | 2008 |
| St.Helens Masonic Hall Limited | Hospitality | 1921 |
| Stag&Sonspubmanagement Ltd | Hospitality | 2025 |
| Stainless Steel RAT Ltd | Wholesale & retail | 2023 |
| Stalk Farm Developments LLP | | 2021 |
| Stallion Communications Ltd | Information & communication | 2024 |
| Standish Wines Ltd | Other services | 2019 |
| Staninski Property Ltd | Real estate | 2026 |
| Staninskitrans Ltd | Transport & logistics | 2015 |
| Stanley Wines Platt Bridge Ltd | Wholesale & retail | 2018 |
| Stapleton Global Ltd | Wholesale & retail | 2025 |
| Starchc Ltd | Health & social care | 2025 |
| Station Bamber Development Limited | Construction | 2020 |
| Station Road Healthcare Ltd | Wholesale & retail | 2024 |
| STE Mullins Smart Repairs Ltd | Wholesale & retail | 2021 |
| Steelcoat Limited | Manufacturing | 2018 |
| Stellar Medical Limited | Health & social care | 2009 |
| STEP10 Studio Ltd | Professional & scientific | 2023 |