| Sheffield Refinishing Supplies Limited | Wholesale & retail | 2009 |
| Sheffield Refractories Limited | Manufacturing | 1976 |
| Sheffield Removals Limited | Transport & logistics | 2025 |
| Sheffield Renewable Services (Holdings) Ltd | Construction | 2021 |
| Sheffield Renewable Services Ltd | Construction | 2023 |
| Sheffield Renovations Ltd | Construction | 2025 |
| Sheffield Rent Ltd | Households | 2018 |
| Sheffield Rentals Limited | Real estate | 2019 |
| Sheffield Roofline and Property Maintenance Limited | Construction | 2017 |
| Sheffield Roofline Limited | Construction | 2021 |
| Sheffield Rugby Union Football Club Limited | Arts & recreation | 2001 |
| Sheffield Sash Ltd | Manufacturing | 2023 |
| Sheffield Scaffolding Services Limited | Construction | 2019 |
| Sheffield Scouts (Holdings) Limited | Administrative & support | 1959 |
| Sheffield Security Systems Ltd | Administrative & support | 2019 |
| Sheffield Service Centre Limited | Wholesale & retail | 2020 |
| Sheffield Signcraft Ltd | Manufacturing | 2006 |
| Sheffield Silver & Waste Recovery Limited | Professional & scientific | 1998 |
| Sheffield Silverware Limited | Wholesale & retail | 2008 |
| Sheffield Skip Hire Ltd | Water & waste | 2011 |
| Sheffield Slim Double Glazing Company Limited | Manufacturing | 2020 |
| Sheffield Smiles Limited | Health & social care | 2023 |
| Sheffield Social Enterprise Network Community Interest Company | Administrative & support | 2018 |
| Sheffield Solar Power Limited | Construction | 2023 |
| Sheffield Solid Surfaces Ltd | Construction | 2015 |
| Sheffield Soroptimist Housing Company Limited | Other services | 1981 |
| Sheffield South East Learning Partnership | Education | 2016 |
| Sheffield South East Trust | Education | 2016 |
| Sheffield Special Steels Limited | Manufacturing | 1991 |
| Sheffield Spintec Ltd | Manufacturing | 2026 |
| Sheffield Station Gym Limited | Arts & recreation | 2020 |
| Sheffield Steel Roller Derby Limited | Arts & recreation | 2011 |
| Sheffield Storage Company Limited | Transport & logistics | 2006 |
| Sheffield Storage Solutions 2008 Limited | Transport & logistics | 2008 |
| Sheffield Student Homes Ltd | Real estate | 2023 |
| Sheffield Sudanese School CIC | Education | 2023 |
| Sheffield Surfacing Ltd | Construction | 1995 |
| Sheffield Tachograph and Tail Lift Services Limited | Wholesale & retail | 1996 |
| Sheffield Tank and Drum Company Limited | Water & waste | 2012 |
| Sheffield Telecom Ltd | Administrative & support | 2017 |
| Sheffield Tenants' Union Limited | Other services | 2025 |
| Sheffield Theatres Trust | Arts & recreation | 1968 |
| Sheffield Therapy Collective Community Interest Company | Health & social care | 2024 |
| Sheffield Timber Company Limited | Manufacturing | 1990 |
| Sheffield Tooling Co Ltd | Manufacturing | 2016 |
| Sheffield Touchless Disinfection Limited | Administrative & support | 2021 |
| Sheffield Transformed Limited | Administrative & support | 2023 |
| Sheffield Transmission Developments Limited | Manufacturing | 2010 |
| Sheffield Transmission Holding Co Ltd | Financial services | 2025 |
| Sheffield Tree Care Limited | Agriculture & fishing | 2009 |
| Sheffield Tyre Limited | Wholesale & retail | 2005 |
| Sheffield United Community Foundation Ltd | Education | 2008 |
| Sheffield Van Hire Limited | Administrative & support | 2010 |
| Sheffield Van Sales Ltd | Wholesale & retail | 2024 |
| Sheffield Video Services Limited | Other services | 2009 |
| Sheffield Vulcans Rufc Ltd | Arts & recreation | 2020 |
| Sheffield Wildcats Roller Hockey Club Limited | Extraterritorial | 2022 |
| Sheffield Wine Week Limited | Professional & scientific | 2025 |
| Sheffield Women's Counselling and Therapy Service Limited | Health & social care | 1989 |
| Sheffield Wood Shed CIC | Manufacturing | 2025 |
| Sheffield Woodcraft Ltd | Manufacturing | 2026 |
| Sheffield Woodland Kindergarten Ltd | Education | 2014 |
| Sheffield Working Women's Opportunities Project | Education | 2005 |
| Sheffield Yoga and Wellness Centre Ltd | Arts & recreation | 2025 |
| Sheffield Young Carers Project | Health & social care | 2010 |
| Sheffield Young Explorers CIC | Education | 2023 |
| Sheffield Young Women's Christian Association | Hospitality | 1999 |
| SHEFFIELD2LET Ltd | Real estate | 2023 |
| Sheffieldtrading Limited | Water & waste | 2023 |
| Sheffiesure Limited | Financial services | 2024 |
| Sheffire Systems Limited | Construction | 2007 |
| Sheffixtech Ltd | Wholesale & retail | 2025 |
| Shefflets Holdings Ltd | Financial services | 2020 |
| Shefflets Limited | Real estate | 2007 |
| Shefflock Auto Locksmith Ltd | Administrative & support | 2024 |
| Shefflock Ltd | Administrative & support | 2016 |
| Shefflock Security Solutions Limited | Administrative & support | 2024 |
| Shefford Carpets Limited | Transport & logistics | 1986 |
| Sheffrack Limited | Manufacturing | 2025 |
| Sheffs Drain Doctor Ltd | Water & waste | 2025 |
| Sheffstay Ltd | Real estate | 2011 |
| Sheffsubs Ltd | Hospitality | 2026 |
| Shefftech Ltd | Manufacturing | 2022 |
| Shefield Recovery Ltd | Transport & logistics | 2025 |
| Shefield Seasons Cafe Ltd | Hospitality | 2025 |
| Shefmed Ltd | Health & social care | 2025 |
| Shehair UK Ltd. | Wholesale & retail | 2007 |
| Shehan P Limited | Hospitality | 2025 |
| Sheila Jacobs Limited | Professional & scientific | 2003 |
| Sheila’S Shack Ltd | Hospitality | 2023 |
| Sheildevox Ltd | Manufacturing | 2026 |
| Shelby Construction PVT Ltd | Construction | 2023 |
| Shelby Properties Limited | Real estate | 2013 |
| Sheldon Inns (Worcester) Limited | Construction | 2013 |
| Sheldon Inns Limited | Hospitality | 2012 |
| Sheldon Lane Flats Co. Limited | Households | 1984 |
| Sheldon Metals Limited | Extraterritorial | 2000 |
| Shelfco (NO. 3272) Limited | Professional & scientific | 1993 |
| Shelfox Builders Ltd | Construction | 2026 |
| Shelon Services Limited | Professional & scientific | 2001 |