| Sheffield Dragon Ltd | Manufacturing | 2020 |
| Sheffield Driveways Ltd | Administrative & support | 2025 |
| Sheffield Eagles 2000 Limited | Arts & recreation | 1999 |
| Sheffield Electric Co Ltd | Construction | 2023 |
| Sheffield Electricians Ltd | Construction | 2018 |
| Sheffield Energy (UK) Limited | Administrative & support | 2012 |
| Sheffield Engine Services Limited | Wholesale & retail | 2003 |
| Sheffield Engineering Company Ltd | Financial services | 2020 |
| Sheffield Engraving Limited | Extraterritorial | 2017 |
| Sheffield Environmental Consultancy Ltd | Construction | 2023 |
| Sheffield Estate Agents Ltd | Real estate | 2024 |
| Sheffield Executive Travel and Airport Transfers Ltd | Transport & logistics | 2016 |
| Sheffield EYE Surgeons Ltd | Health & social care | 2013 |
| Sheffield Fabrications Limited | Manufacturing | 2022 |
| Sheffield Fans Derby Limited | Arts & recreation | 2012 |
| Sheffield Financial Services Limited | Wholesale & retail | 1993 |
| Sheffield Floor Sanding Company Ltd | Construction | 2013 |
| Sheffield Flooring Limited | Construction | 2014 |
| Sheffield Flooring Solutions Ltd | Wholesale & retail | 2008 |
| Sheffield Flourish | Health & social care | 2012 |
| Sheffield Folk Projects CIC | Education | 2026 |
| Sheffield Foot Clinic Limited | Health & social care | 2025 |
| Sheffield Forgemasters Engineering Limited | Manufacturing | 2003 |
| Sheffield Forgemasters International Limited | Manufacturing | 2003 |
| Sheffield Fresh Food Limited | Wholesale & retail | 2023 |
| Sheffield Fruit Trees Ltd. | Health & social care | 2012 |
| Sheffield Garage Conversions Limited | Administrative & support | 2017 |
| Sheffield Gauge Plate Limited | Manufacturing | 2003 |
| Sheffield Glass Company Limited | Construction | 2019 |
| Sheffield Goldsmiths Company Limited | Wholesale & retail | 2016 |
| Sheffield Hallam Innovation and Enterprise Limited | Professional & scientific | 2002 |
| Sheffield Hallam Students’ Union | Education | 2024 |
| Sheffield Hallam University Enterprises Ltd | Professional & scientific | 1987 |
| Sheffield Hallam University Services Limited | Education | 2025 |
| Sheffield Hallamshire Rufc Ltd | Arts & recreation | 2014 |
| Sheffield Handypro Ltd | Construction | 2025 |
| Sheffield Hardware Hackers and Makers CIC | Arts & recreation | 2015 |
| Sheffield Heating Solutions Limited | Construction | 2014 |
| Sheffield Homes Limited | Real estate | 2023 |
| Sheffield Honey Limited | Manufacturing | 2010 |
| Sheffield Hospitals Charity | Health & social care | 2016 |
| Sheffield House of Intercession | Other services | 2025 |
| Sheffield Indoor Cricket Centre Limited | Arts & recreation | 2012 |
| Sheffield Insulations Limited | Extraterritorial | 1957 |
| Sheffield International College | Professional & scientific | 2005 |
| Sheffield International Freight Terminal Limited | Transport & logistics | 1975 |
| Sheffield International Venues Limited | Arts & recreation | 1988 |
| Sheffield Investments Ltd | Real estate | 2018 |
| Sheffield Jazz | Arts & recreation | 2008 |
| Sheffield Kitchen Company Limited | Construction | 2019 |
| Sheffield Knife Sharpening Ltd | Manufacturing | 2019 |
| Sheffield Language Centre Ltd | Education | 2017 |
| Sheffield Lettings Limited | Real estate | 2005 |
| Sheffield Live Ltd | Information & communication | 2003 |
| Sheffield Loft Conversions Limited | Manufacturing | 2022 |
| Sheffield LUX Travel & Chauffeurs Ltd | Transport & logistics | 2025 |
| Sheffield Lyceum Theatre Trust Limited | Arts & recreation | 1986 |
| Sheffield Machine Knife Limited | Manufacturing | 2008 |
| Sheffield Medical Products Limited | Professional & scientific | 2007 |
| Sheffield Mela CIC | Education | 2023 |
| Sheffield Mencap | Health & social care | 1996 |
| Sheffield Metallurgical and Engineering Association | Other services | 1993 |
| Sheffield Metallurgical Services Ltd | Professional & scientific | 2020 |
| Sheffield Mind Limited | Health & social care | 1977 |
| Sheffield Mint Ltd | Manufacturing | 2026 |
| Sheffield Mobility Ltd | Manufacturing | 2020 |
| Sheffield Mobility Solutions Ltd | Manufacturing | 2020 |
| Sheffield MOT Centre Ltd | Wholesale & retail | 2024 |
| Sheffield Motorcycles (OFF Road) Limited | Wholesale & retail | 2020 |
| Sheffield Museums Trust | Arts & recreation | 2020 |
| Sheffield Music Academy | Education | 2011 |
| Sheffield Music Co-Operative Limited | Information & communication | 2012 |
| Sheffield Narrowboats Limited | Transport & logistics | 2006 |
| Sheffield Native Plants Ltd | Wholesale & retail | 2025 |
| Sheffield Neuro Physio Limited | Health & social care | 2025 |
| Sheffield Orthodontic Laboratories Limited | Manufacturing | 1979 |
| Sheffield Orthopaedic Specialists Ltd | Health & social care | 2024 |
| Sheffield Orthopaedics Limited | Health & social care | 2003 |
| Sheffield Pain Opinion Limited | Health & social care | 2016 |
| Sheffield Parent Carer Forum | Other services | 2010 |
| Sheffield Park Hotel Ltd | Hospitality | 2011 |
| Sheffield Park Hotel Property Ltd | Hospitality | 2011 |
| Sheffield Phoenix Press Limited | Information & communication | 2004 |
| Sheffield Plastering Services Ltd | Construction | 2015 |
| Sheffield Plumbing and Heating Ltd | Construction | 2014 |
| Sheffield Plumbing Services Ltd | Construction | 2016 |
| Sheffield Poultry Limited | Wholesale & retail | 2011 |
| Sheffield Practice Limited | Health & social care | 2009 |
| Sheffield Precision Medical Ltd | Manufacturing | 2010 |
| Sheffield Pregnancy Counselling Support | Professional & scientific | 2009 |
| Sheffield Prestige Limited | Wholesale & retail | 2011 |
| Sheffield Private Tutors Ltd | Education | 2020 |
| Sheffield Properties Limited | Real estate | 2002 |
| Sheffield Property Association Limited | Other services | 2018 |
| Sheffield Property Developments Ltd | Real estate | 2016 |
| Sheffield Property Services Ltd | Construction | 2020 |
| Sheffield Property to LET Ltd | Real estate | 2018 |
| Sheffield Psychological Services Ltd | Health & social care | 2017 |
| Sheffield Rape and Sexual Abuse Centre | Other services | 2012 |
| Sheffield Recruitment Specialists Ltd | Administrative & support | 2022 |