| Sheff Halal Butchers Limited | Wholesale & retail | 2023 |
| Sheff Households Ltd | Wholesale & retail | 2024 |
| Sheff Made Ltd | Wholesale & retail | 2021 |
| Sheff Minimarket S4 Limited | Wholesale & retail | 2025 |
| Sheff Repointing Co. Ltd | Construction | 2026 |
| Sheff Smash Limited | Hospitality | 2026 |
| Sheff Smile Ltd | Hospitality | 2025 |
| Sheff Trade Limited | Administrative & support | 2022 |
| Sheff Tutor Ltd. | Information & communication | 2022 |
| Sheffcare Limited | Health & social care | 1990 |
| Sheffcare Services Limited | Health & social care | 2021 |
| Sheffcat Limited | Wholesale & retail | 2009 |
| Sheffclad Ltd | Construction | 2025 |
| Sheffclean Ltd | Administrative & support | 2022 |
| Sheffield & District Junior Football League Community Interest Company | Arts & recreation | 2015 |
| Sheffield & Hallamshire Motor Club Limited | Arts & recreation | 1951 |
| Sheffield & London Shipping Limited | Transport & logistics | 1981 |
| Sheffield 2 Limited | Professional & scientific | 2019 |
| Sheffield 3D Prints Limited | Extraterritorial | 2021 |
| Sheffield 5 Limited | Professional & scientific | 2019 |
| Sheffield Adventure Film Festival CIC | Information & communication | 2025 |
| Sheffield African Caribbean Mental Health Association Limited | Health & social care | 1996 |
| Sheffield Aggregates & Haulage Ltd | Transport & logistics | 2024 |
| Sheffield AI Limited | Information & communication | 2024 |
| Sheffield Air Conditioning Services Ltd | Construction | 2020 |
| Sheffield Alloy Steel Company Limited | Extraterritorial | 1997 |
| Sheffield Alternative Provision for Education Ltd | Education | 2019 |
| Sheffield Analytical Services Limited | Extraterritorial | 1995 |
| Sheffield and District Association of Unitarian and Free Christian Churches (Incorporated) (the) | Other services | 1942 |
| Sheffield and District Fair Play League Limited | Arts & recreation | 2020 |
| Sheffield and District Reform Jewish Congregation | Other services | 2008 |
| Sheffield and Hallamshire County Football Association Limited | Arts & recreation | 2001 |
| Sheffield Angels Ltd | Financial services | 2025 |
| Sheffield Apartments Limited | Real estate | 2015 |
| Sheffield Arrhythmia Centre Limited | Health & social care | 2017 |
| Sheffield Artist Maker Exchange CIC | Manufacturing | 2024 |
| Sheffield Asbestos Surveys Ltd | Construction | 2009 |
| Sheffield Association for the Voluntary Teaching of English | Education | 1999 |
| Sheffield Auto Centre Ltd | Wholesale & retail | 2017 |
| Sheffield Auto FX Limited | Wholesale & retail | 2012 |
| Sheffield Auto Services Ltd | Wholesale & retail | 2018 |
| Sheffield Auto Spares Ltd | Wholesale & retail | 2025 |
| Sheffield Autocare Limited | Wholesale & retail | 2018 |
| Sheffield Autogates Limited | Manufacturing | 2015 |
| Sheffield Baby Massage Instructor Limited | Education | 2025 |
| Sheffield Bathroom Company Ltd | Construction | 2023 |
| Sheffield Bathroom Solutions Ltd | Wholesale & retail | 2025 |
| Sheffield Bazar Ltd | Wholesale & retail | 2023 |
| Sheffield Beauty Lounge Ltd | Other services | 2025 |
| Sheffield Beer Museum CIC | Arts & recreation | 2025 |
| Sheffield Beerworks EOT Ltd | Financial services | 2024 |
| Sheffield Bespoke Driveways Limited | Construction | 2021 |
| Sheffield Billiards Ltd | Arts & recreation | 2019 |
| Sheffield Boiler Installations Ltd | Construction | 2025 |
| Sheffield Boiler Services Ltd | Other services | 2025 |
| Sheffield Boilers Ltd | Construction | 2021 |
| Sheffield Botanical Gardens Limited | Professional & scientific | 2004 |
| Sheffield Braider Ltd | Other services | 2025 |
| Sheffield Breakdown & Mobile Tyres Ltd | Wholesale & retail | 2026 |
| Sheffield Building Company Limited | Construction | 2025 |
| Sheffield BUS & Truck Limited | Wholesale & retail | 2014 |
| Sheffield Business Centre Limited | Construction | 1994 |
| Sheffield Business Park Limited | Real estate | 1995 |
| Sheffield Calibration Services Ltd | Professional & scientific | 2015 |
| Sheffield Canoe Club | Arts & recreation | 2017 |
| Sheffield Car Centre Ltd | Wholesale & retail | 2013 |
| Sheffield Car Charging Limited | Construction | 2021 |
| Sheffield Car Company Ltd | Wholesale & retail | 2020 |
| Sheffield Car Company Sales Ltd | Wholesale & retail | 2025 |
| Sheffield Car Parts Limited | Wholesale & retail | 2018 |
| Sheffield Car Shop Limited | Wholesale & retail | 2011 |
| Sheffield Care and Support Association Ltd | Health & social care | 2026 |
| Sheffield Care Association Ltd | Health & social care | 2021 |
| Sheffield Cash 4 Cars Limited | Administrative & support | 2013 |
| Sheffield Cathedral Enterprises Limited | Wholesale & retail | 2015 |
| Sheffield Central Counselling Limited | Professional & scientific | 2013 |
| Sheffield Chamber of Commerce and Industry | Other services | 1899 |
| Sheffield Cheese Limited | Manufacturing | 2010 |
| Sheffield Chinese Association | Education | 2004 |
| Sheffield Chinese Community Centre | Health & social care | 2012 |
| Sheffield Citizens Advice and Law Centre | Information & communication | 2013 |
| Sheffield City Centre BID Limited | Other services | 2015 |
| Sheffield City Living Ltd | Real estate | 2015 |
| Sheffield City Opportunities CIC | Information & communication | 2025 |
| Sheffield City Properties Limited | Real estate | 2008 |
| Sheffield City Trust | Arts & recreation | 1987 |
| Sheffield Co-Operative Ventures Limited | Financial services | 1984 |
| Sheffield Coated Stone Limited | Wholesale & retail | 2010 |
| Sheffield Construction Ltd | Construction | 2025 |
| Sheffield Countryside Conservation Trust | Other services | 2003 |
| Sheffield Creative Interiors Limited | Construction | 2019 |
| Sheffield Crucible Productions Limited | Arts & recreation | 2012 |
| Sheffield Culture Consortium | Arts & recreation | 2020 |
| Sheffield Democratic Kurdish People's Assembly Limited | Education | 2021 |
| Sheffield Detailing Services Ltd | Professional & scientific | 1999 |
| Sheffield Developments Ltd | Construction | 2016 |
| Sheffield Digital Limited | Other services | 2015 |
| Sheffield Distillery Limited | Manufacturing | 2017 |
| Sheffield Docfest Limited | Other services | 2021 |
| Sheffield Double Glazing Company Ltd | Construction | 2020 |