| Enkay Mandisym Ltd | Hospitality | 2022 |
| Enke Limited | Hospitality | 2019 |
| Enki Collective Limited | Arts & recreation | 2020 |
| Enlighten Sheffield Limited | Education | 2022 |
| Enlightened Spirit Ltd | Wholesale & retail | 2022 |
| Enlite Education & Technology Ltd | Information & communication | 2020 |
| Enon Films Limited | Information & communication | 2012 |
| Enorth Limited | Information & communication | 2004 |
| Enosys Limited | Information & communication | 2024 |
| Enovix Group Ltd | Wholesale & retail | 2024 |
| ENP Construction Ltd | Construction | 2023 |
| Enrich Education Limited | Education | 2014 |
| Enrichment for the Elderly Ltd | Education | 2015 |
| Ensco 1427 Limited | Professional & scientific | 2021 |
| Ensco 1462 Limited | Professional & scientific | 2022 |
| Enspan Limited | Administrative & support | 2011 |
| ENT Medical and Surgical Ltd | Health & social care | 2026 |
| ENT Sheffield Limited | Health & social care | 2012 |
| Ente Peedika Company Ltd | Wholesale & retail | 2026 |
| Entech International Ltd | Arts & recreation | 2015 |
| Enter Area Limited | Real estate | 2006 |
| Enterluxe Corporation Ltd | Wholesale & retail | 2025 |
| Enterprise AMA Ltd | Hospitality | 2023 |
| Enterprise Compliance Limited | Professional & scientific | 2008 |
| Enterprise Conformance Limited | Professional & scientific | 2008 |
| Enterprise Financial Services Ltd | Financial services | 2003 |
| Enterprise International Solutions Ltd | Professional & scientific | 2026 |
| Enterprise Management Solutions Ltd | Wholesale & retail | 2023 |
| Enterprise Property & Rental Services Limited | Real estate | 2021 |
| Enterprises JDJ Ltd | Hospitality | 2024 |
| Entersys Limited | Administrative & support | 2009 |
| Entertainment Sports Agency Limited | Professional & scientific | 2013 |
| Entire Solutions Ltd | Construction | 2019 |
| Entire Trades Solutions Limited | Manufacturing | 2020 |
| Entobrain Ltd | Information & communication | 2025 |
| Entreprenlaw Group Limited | Professional & scientific | 2023 |
| Entropica Ltd | Professional & scientific | 2013 |
| Entwistle Estates Ltd | Real estate | 2024 |
| Enva Lewis Ltd | Arts & recreation | 2024 |
| Enviraflex Ltd | Manufacturing | 2023 |
| Enviroload Contract Services Ltd | Transport & logistics | 2017 |
| Enviroload Waste Services Ltd | Water & waste | 2017 |
| Enviromonitoring Ltd | Professional & scientific | 2025 |
| Enviromove Services Ltd | Construction | 2022 |
| Environmental Consultation & Equipment Ltd | Administrative & support | 2019 |
| Environmental Cooling & Heating Limited | Construction | 2025 |
| Environmental Recovery Incorporated Limited | Energy | 1991 |
| Environstruct Solutions Limited | Professional & scientific | 2024 |
| Envirotec Hygiene Limited | Administrative & support | 2020 |
| Envol DE Retz UK Limited | Agriculture & fishing | 2024 |
| Enwood Trade Limited | Wholesale & retail | 2025 |
| Enzkconnect Ltd | Information & communication | 2019 |
| Enzo Acquisitions Limited | Other services | 2022 |
| Enzo Catering Ltd | Hospitality | 2025 |
| Enzo IS Burning Limited | Information & communication | 2024 |
| Eosh Consulting Limited | Administrative & support | 2017 |
| Eostrix Finance Limited | Financial services | 2024 |
| EP & BTW Flooring Limited | Wholesale & retail | 2022 |
| EP Coaching Ltd | Education | 2023 |
| Ep&G Ltd | Hospitality | 2023 |
| Epaq Systems Limited | Professional & scientific | 2006 |
| Epbeer Limited | Hospitality | 2014 |
| Epbm Limited | Households | 2025 |
| EPC Yorkshire Limited | Professional & scientific | 2014 |
| Epgl EBT Trustee Limited | Administrative & support | 2017 |
| Ephratah Seed Group Ltd | Extraterritorial | 2023 |
| EPI Genesys Limited | Information & communication | 2007 |
| EPI Lettings Limited | Households | 2021 |
| Epic Meat and Fruit Merchants Ltd | Wholesale & retail | 2020 |
| Epicheírisi Ltd | Administrative & support | 2020 |
| Epiconsult Limited | Information & communication | 2018 |
| Epics (UK) Limited | Information & communication | 2007 |
| Epidermic Tattoo Ltd | Other services | 2015 |
| Eplom Limited | Manufacturing | 2013 |
| Eplus Services Limited | Information & communication | 2018 |
| Epodal Limited | Professional & scientific | 2019 |
| EPS Investments (Cartergate) Limited | Real estate | 2023 |
| EPS Investments (Europa Green) Limited | Real estate | 2021 |
| EPS Investments (ST Cuthbert's Way) Limited | Real estate | 2018 |
| Epsee Ltd | Professional & scientific | 2016 |
| Epworth Meadows Limited | Real estate | 2018 |
| Epworth Rose Ltd | Construction | 2012 |
| EQA International Limited | Administrative & support | 2000 |
| Equal Communities Ltd | Administrative & support | 1998 |
| Equal Force Security Services Limited | Administrative & support | 2020 |
| Equalcite Limited | Arts & recreation | 1997 |
| Equanimity 1 Ltd | Hospitality | 2024 |
| Equi-Trek Limited | Manufacturing | 2012 |
| Equinox Publishing Limited | Information & communication | 2003 |
| EQUIP2SPEAK Ltd | Health & social care | 2019 |
| Equiplus Intercontinental Limited | Health & social care | 2003 |
| Equiplus International Limited | Administrative & support | 1999 |
| Equipserv UK Limited | Manufacturing | 2008 |
| Equitec Asset Management Ltd | Real estate | 2010 |
| Equity Value Limited | Professional & scientific | 2006 |
| ER Bullion Ltd | Manufacturing | 2026 |
| ER Hair Ltd | Other services | 2024 |
| ERA Consultancy Limited | Information & communication | 1997 |
| Erasteel Alloys UK Ltd | Wholesale & retail | 1984 |
| Erasteel Stubs Limited | Manufacturing | 2000 |