| Emporiumdirect Ltd | Wholesale & retail | 2025 |
| Empower Life Supported Living Limited | Health & social care | 2023 |
| Empower Therapy & Homecare Ltd | Health & social care | 2024 |
| Empower World Ltd | Education | 2020 |
| Empower Wrestling Ltd | Education | 2021 |
| Empowering Education International Limited | Professional & scientific | 2023 |
| Empra Limited | Professional & scientific | 2006 |
| Empress Tree Ltd | Other services | 2024 |
| Empressa Limited | Professional & scientific | 2025 |
| Emrh Consult Ltd | Professional & scientific | 2023 |
| EMS Artist Management Limited | Administrative & support | 2021 |
| EMS Elec Ltd | Construction | 2015 |
| EMS MD Ltd | Manufacturing | 2022 |
| Emsc (UK) Limited | Manufacturing | 2001 |
| Emsc Global Limited | Manufacturing | 2013 |
| EMT Talent Limited | Administrative & support | 2025 |
| EN Living Support Limited | Health & social care | 2018 |
| EN Route Pet Transport Ltd | Transport & logistics | 2024 |
| En:Mapping Limited | Professional & scientific | 2012 |
| En:Procure Limited | Other services | 2014 |
| Enable Corporate Strategies Ltd | Financial services | 2010 |
| Enable Space Ltd | Administrative & support | 2014 |
| Enable Unity CIC | Arts & recreation | 2018 |
| Enactus Sheffield Limited | Administrative & support | 2006 |
| Enanda Limited | Information & communication | 2015 |
| ENB Haulage Ltd | Transport & logistics | 2024 |
| Enby Recruitment Limited | Administrative & support | 2023 |
| Enchanted Travel Limited | Administrative & support | 2023 |
| Enclave M J Ltd | Hospitality | 2026 |
| Encompass Enterprises Limited | Professional & scientific | 2012 |
| Encora Systems Limited | Information & communication | 2019 |
| END C C Ltd | Information & communication | 2025 |
| END Time Evangelism Ministries | Other services | 2007 |
| Endcliffe Grove Associates Limited | Information & communication | 2011 |
| Endcliffe Orchestra Concerts Limited | Arts & recreation | 2022 |
| Endcliffe Rise Court RTM Company Limited | Households | 2016 |
| Endcliffe Trading Ltd | Wholesale & retail | 2025 |
| Endcliffe Vale Lessees Limited | Households | 1970 |
| Endcrown Limited | Professional & scientific | 2001 |
| Endeavour Psychological Services Limited | Health & social care | 2017 |
| Endeavour Training Limited | Education | 1974 |
| Endoz Ltd | Health & social care | 2022 |
| Endpoint Esports Ltd | Arts & recreation | 2016 |
| Endurance Financial Limited | Financial services | 2015 |
| Eneacfd Limited | Professional & scientific | 2023 |
| Eneas Limited | Professional & scientific | 2013 |
| Eneraqua Technologies PLC | Professional & scientific | 2021 |
| Energize Sales & Marketing Limited | Professional & scientific | 2023 |
| Energy Clever (UK) Limited | Construction | 2013 |
| Energy Flow Electrical Ltd | Construction | 2019 |
| Energy Grants Ltd | Professional & scientific | 2025 |
| Energy Installer Limited | Professional & scientific | 2025 |
| Energy Maintenance Limited Limited | Professional & scientific | 2022 |
| Energy One Ltd | Construction | 2024 |
| Energy Smart Solutions Limited | Construction | 2009 |
| Energy Solutions SY Ltd | Manufacturing | 2026 |
| Energy Strategy and Policy Consulting Limited | Administrative & support | 2008 |
| Energy Systems UK Limited | Information & communication | 2001 |
| Energy Technical Systems Ltd | Professional & scientific | 2025 |
| Energy Training Centre Limited | Education | 2011 |
| Energy Upgrade Solutions Ltd | Manufacturing | 2024 |
| Energy Vision Ltd | Professional & scientific | 2012 |
| Energyform Limited | Extraterritorial | 2001 |
| Energyshire Limited | Energy | 2019 |
| Energytest Holdings Limited | Financial services | 2019 |
| Energytest Ltd | Construction | 2007 |
| Enfield Consultants Ltd | Professional & scientific | 2024 |
| Engage & Share Limited | Information & communication | 2011 |
| Engage+Engineer Ltd | Information & communication | 2023 |
| Engagent Limited | Professional & scientific | 2022 |
| Engel Europa International Limited | Manufacturing | 1983 |
| Engeniare Holdings Limited | Financial services | 2024 |
| Engeniare Limited | Professional & scientific | 2014 |
| Engine House Limited | Administrative & support | 2016 |
| Engineerable UK Ltd | Manufacturing | 2021 |
| Engineering (UK) Limited | Wholesale & retail | 2004 |
| Engineering 1 Solutions Limited | Professional & scientific | 2025 |
| Engineering CFD Ltd | Professional & scientific | 2015 |
| Engineering Integrity Society | Other services | 1985 |
| Engineering of Surgical Ltd | Wholesale & retail | 2024 |
| Engineering Supply Solutions Ltd | Manufacturing | 2014 |
| Engineering Transport Solutions Ltd | Wholesale & retail | 2026 |
| England Boxing Limited | Professional & scientific | 1993 |
| England Harman Pogson Limited | Real estate | 2010 |
| English Chain Company Limited | Manufacturing | 1947 |
| English Chain Direct Limited | Extraterritorial | 2014 |
| English Land and Estates Limited | Real estate | 2000 |
| English Oak (UK) Limited | Administrative & support | 2004 |
| English Touring Opera Limited | Arts & recreation | 1979 |
| Engraveandembrace Ltd | Wholesale & retail | 2025 |
| Engrus Consulting Group Ltd | Professional & scientific | 2024 |
| Enhanced Energy Solutions Ltd | Construction | 2015 |
| Enid Wing Trading Ltd | Wholesale & retail | 2024 |
| Enigma Climbing Products Ltd | Manufacturing | 2018 |
| Enigma Climbing Services Ltd | Administrative & support | 2024 |
| Enigma Signs Limited | Wholesale & retail | 2009 |
| Enixie Ltd | Manufacturing | 2026 |
| Enjay Creative Limited | Professional & scientific | 2011 |
| Enjoy Making Music CIC | Education | 2026 |
| Enkaised Ltd | Wholesale & retail | 2026 |