| Iain Boyle Heating & Plumbing Ltd. | Construction | 2007 |
| IG Photography Ltd | Professional & scientific | 2021 |
| IN and OUT Garden Houses Ltd | Construction | 2020 |
| Incorporated Society of Registered Naturopaths Limited | Health & social care | 1934 |
| Inner Beat Coaching Ltd | Professional & scientific | 2026 |
| Innerleithen Steak Limited | Hospitality | 2026 |
| Into Supply Limited | Professional & scientific | 2017 |
| Irvine Electrical Edinburgh Ltd | Other services | 2017 |
| Irvine Plant Limited | Construction | 2017 |
| J&D Worldwide Ltd | Wholesale & retail | 2023 |
| J&L Origins Ltd | Wholesale & retail | 2025 |
| Jackaranda Limited | Real estate | 2026 |
| Jackie Denholm Moore Ltd | Other services | 2018 |
| Jarvie Sales Solutions Ltd | Wholesale & retail | 2026 |
| JBM Estate Agents Limited | Real estate | 2011 |
| JC and Sons Construction and Maintenance Ltd | Construction | 2021 |
| JCS Autocare Limited | Agriculture & fishing | 2025 |
| Jerryht Ltd | Transport & logistics | 2025 |
| JLS Peebles Limited | Administrative & support | 2024 |
| John Dragon COX Ltd | Real estate | 2019 |
| JRD Motors (2011) Limited | Wholesale & retail | 2011 |
| Jura Consultants Limited | Professional & scientific | 1999 |
| JWB Windows & Conservatories Ltd | Construction | 2017 |
| Kelmor Ltd. | Real estate | 2012 |
| Kenny Gibson Limited | Extraterritorial | 2009 |
| Kerfield Limited | Professional & scientific | 2014 |
| Kerr Meter Consultancy Limited | Professional & scientific | 2016 |
| KH Consultancy Projects Limited | Information & communication | 2013 |
| Kindness Property Group Limited | Real estate | 2024 |
| Kingsmeadows Social Enterprises Ltd. | Hospitality | 2007 |
| Kingsmuir Construction (SCO) Ltd | Construction | 2024 |
| KM Property Investment Ltd | Real estate | 2019 |
| Kuro Tabad Limited | Information & communication | 2022 |
| L&J Total Clean Ltd | Administrative & support | 2019 |
| Laird Farming Company | Administrative & support | 2010 |
| Langshaw Homes Limited | Construction | 2014 |
| Law ALL Trades Ltd | Construction | 2021 |
| Lawson's Premier Ground Care Ltd | Administrative & support | 2010 |
| LBN Financial Limited | Financial services | 2026 |
| LCM Rehab Ltd | Health & social care | 2019 |
| Learmonth Services Ltd | Professional & scientific | 2023 |
| Leetside Limited | Professional & scientific | 2013 |
| Leopard Services Limited | Administrative & support | 2024 |
| Linfaws Consulting Limited | Professional & scientific | 2011 |
| Live Learn Earn C.I.C. | Other services | 2020 |
| Llama-Ra Films Ltd | Information & communication | 2013 |
| Lmjc Ltd | Real estate | 2023 |
| Lodnok Property Ltd | Construction | 2019 |
| Longcote Herbs Ltd | Health & social care | 2023 |
| Luckman Property Ltd | Real estate | 2020 |
| M2 Scotland Ltd | Construction | 2020 |
| Mandarin Garden Peebles Limited | Hospitality | 2018 |
| Manor Consultancy Services Ltd | Agriculture & fishing | 2018 |
| Maryanne Johnston Ltd | Administrative & support | 2016 |
| Matt Davies Environmental Consulting LLP | | 2020 |
| Mclaren Building and Electrical Services Limited | Construction | 2010 |
| Mcnab Consulting Engineers Ltd | Professional & scientific | 2020 |
| Meldon Seeds Ltd | Agriculture & fishing | 2020 |
| Messrs Douglas-Damhead Farm | | 1987 |
| Millson Property Ltd | Construction | 2025 |
| Milne & Pickles Limited | Manufacturing | 2021 |
| Milspec Consultants Ltd | Professional & scientific | 2020 |
| MJC Surveyors Ltd | Professional & scientific | 2012 |
| Moffat Hotel Limited | Hospitality | 2020 |
| Moffat Operations Limited | Hospitality | 2020 |
| Mominmahir&Co Limited | Wholesale & retail | 2026 |
| Montmer Limited | Real estate | 2016 |
| Moto Carbon Innovation Ltd | Manufacturing | 2020 |
| MOY Mackay Gallery Limited | Wholesale & retail | 2012 |
| Mrc's Peebles Limited | Hospitality | 2026 |
| MW & AWR Ireland Ltd | Real estate | 2019 |
| Mycoessentials Ltd | Wholesale & retail | 2024 |
| N Plus 1 Holdings Limited | Financial services | 2026 |
| Nabab (Peeb) Ltd | Hospitality | 2024 |
| NAE Pasaran Limited | Information & communication | 2015 |
| Natalie Jade Ltd | Professional & scientific | 2024 |
| Natural Capital Brokers Limited | Professional & scientific | 2025 |
| Natural Capital Holdings Limited | Wholesale & retail | 2023 |
| Neidpath Inn Peebles Ltd | Hospitality | 2024 |
| Neidpath Inn Restaurant and PUB Ltd | Hospitality | 2019 |
| Newburgh Holiday Lets Ltd. | Hospitality | 2017 |
| Next GEN Competitions Ltd | Wholesale & retail | 2023 |
| Nine Mile Burn Car Sales Ltd | Wholesale & retail | 2024 |
| NLT Property Limited | Real estate | 2024 |
| Nomad Beat Ltd. | Arts & recreation | 2009 |
| North Route Systems Ltd | Information & communication | 2026 |
| North Star - Learning and Performance Consultants Ltd | Professional & scientific | 2019 |
| North Star Commercial Property Ltd | Real estate | 2023 |
| North Star Origin Ltd | Real estate | 2024 |
| Northeast Creative Ltd | Information & communication | 2009 |
| Northgate ST Barbers Peebles Ltd | Other services | 2024 |
| Novo Design Studios Ltd | Professional & scientific | 2002 |
| Novo Developments Ltd | Construction | 2016 |
| Novo Holdco Ltd | Financial services | 2026 |
| Novo Property Ltd | Real estate | 2018 |
| Oakstone Marketing Group Ltd | Professional & scientific | 2026 |
| Olim Communications Limited | Professional & scientific | 2021 |
| Oliver Portfolio Ltd | Real estate | 2018 |
| Omni Gym Limited | Arts & recreation | 2013 |
| Opion Ua-Uk Ltd | Wholesale & retail | 2025 |