| Content Interiors Ltd | Wholesale & retail | 2026 |
| Cooper Coaching Consultancy Ltd | Professional & scientific | 2013 |
| Coorie Lifestyle Ltd | Wholesale & retail | 2025 |
| Cormack Consultancy Baltic Limited | Professional & scientific | 2006 |
| Courthouse Ltd | Construction | 2017 |
| Craig Howie Golf Limited | Arts & recreation | 2020 |
| Crossfit Skirmish Ltd | Arts & recreation | 2017 |
| Crown Roofing Scot Limited | Construction | 2022 |
| CSR Holdings Limited | Hospitality | 2012 |
| Custom Kitchens & Bathrooms (Peebles) Ltd | Manufacturing | 2023 |
| D. Caird - Howford Farm | | 1987 |
| Dablam Consulting Limited | Professional & scientific | 2024 |
| Daleway Limited | Real estate | 1997 |
| Darren Woodhead Limited | Arts & recreation | 2015 |
| David B Carlisle (Pension Trustees) Limited | Administrative & support | 2001 |
| David Dodds Associates Ltd | Professional & scientific | 2012 |
| David Harrison Limited | Wholesale & retail | 1956 |
| David Neilson Agencies Limited | Wholesale & retail | 1986 |
| DAX Worldwide Ltd | Hospitality | 2025 |
| Daymaker HCM Ltd | Information & communication | 2025 |
| Debasers Films Ltd | Information & communication | 2017 |
| Debasers Filums Limited | Information & communication | 2012 |
| Deje Systems Limited | Information & communication | 2021 |
| DH Mechanical Services Ltd | Professional & scientific | 2016 |
| Dragonfly Crystals & Gifts Ltd | Wholesale & retail | 2023 |
| Drew Caldwell Joinery & Construction Ltd | Construction | 2022 |
| DS Joinery & Construction Ltd | Construction | 2023 |
| Dufferin Software Limited | Information & communication | 2023 |
| DW Timberframe Ltd | Construction | 2022 |
| Dysguise Diagnostics Ltd | Information & communication | 2023 |
| Dysguise Limited | Education | 2010 |
| Earrach Limited | Agriculture & fishing | 2026 |
| Earthtech (E-Waste) Ltd | Water & waste | 2025 |
| Easyset Ltd | Information & communication | 2025 |
| Ecofitautocare Ltd | Wholesale & retail | 2025 |
| Eddleston Innovation Limited | Professional & scientific | 2000 |
| Eden Support Service Ltd | Administrative & support | 2023 |
| Edimed Developments Ltd | Financial services | 2021 |
| Edinburgh Commercial Project Management Ltd | Construction | 2021 |
| Edinburgh Project Management Ltd | Construction | 2005 |
| Ellie Clarke Investment Ltd | Construction | 2023 |
| Embodied Rose Ltd | Wholesale & retail | 2025 |
| Emfrab Properties Limited | Real estate | 2025 |
| Enhance Support + Care LLP | | 2016 |
| Enviraware Limited | Manufacturing | 1989 |
| Environmental Research and Development Ltd. | Professional & scientific | 2006 |
| Eshiels Holdings Ltd | Hospitality | 2025 |
| Eskside Motor Factors Limited | Wholesale & retail | 2011 |
| Eureka Golf Swing Ltd | Education | 2020 |
| FAB Adventures Ltd | Education | 2011 |
| Facilities Management Scotland Limited | Construction | 2017 |
| Fairmile Plumbing Limited | Construction | 1994 |
| Fallhills Fencing Ltd | Agriculture & fishing | 2012 |
| FAR FAR Away Ltd | Real estate | 2023 |
| FB Projects Ltd | Energy | 2023 |
| Federation Support Services Ltd | Administrative & support | 2007 |
| Figgys PAD Ltd | Wholesale & retail | 2025 |
| Financial Adviser Acquisitions Ltd | Professional & scientific | 2025 |
| Finlayson Property LLP | | 2008 |
| Finnie and Walkinshaw | | 1983 |
| Fiona Henderson School of Dance Ltd | Education | 2024 |
| First Nursery Limited | Health & social care | 2000 |
| Franco's Pizzeria Ristorante Ltd | Hospitality | 2019 |
| Friends of Leadburn Community Woodland | Agriculture & fishing | 2005 |
| G M Land Solutions Ltd | Administrative & support | 2015 |
| G Mathison Commercial Ltd. | Real estate | 2013 |
| Gala Life Limited | Information & communication | 2009 |
| Gareth Easton Photography Ltd | Professional & scientific | 2015 |
| GHS Consult Ltd | Professional & scientific | 2025 |
| Glenbield Limited | Real estate | 2009 |
| Glenormiston Estates Limited | Construction | 2006 |
| Glentress Homes Limited | Construction | 2007 |
| Gordon Graham Design & Build Ltd | Construction | 2008 |
| Gotbeans Limited | Hospitality | 2017 |
| GP Finestar Ltd | Wholesale & retail | 2026 |
| Gradwell Contracting Ltd | Construction | 2023 |
| Granjock Limited | Real estate | 1994 |
| Gray Wolf Consulting Ltd | Professional & scientific | 2014 |
| Greenways Analysis Ltd | Information & communication | 2022 |
| Gri-Trading Limited | Wholesale & retail | 2021 |
| GSM Electrical Services Ltd | Construction | 2026 |
| GUS Gillies Cycle Services Limited | Professional & scientific | 2025 |
| H Stewart Construction Ltd | Construction | 2016 |
| Habao Nails Limited | Other services | 2025 |
| Hair by Studio One Limited | Wholesale & retail | 2012 |
| Halion Ltd. | Real estate | 2001 |
| Hamilton Ag-Tech Advisory Ltd. | Professional & scientific | 2006 |
| Hamilton Property (Peebles) Limited | Real estate | 2024 |
| Haystoun Financial Services Ltd. | Other services | 2018 |
| Haystoun Property Services Ltd. | Other services | 2018 |
| High-Speed Universal Broadband Services C.I.C. | Information & communication | 2013 |
| Hillside Agency Limited | Professional & scientific | 2012 |
| Hillside Outside Ltd | Arts & recreation | 2012 |
| HKD Solutions Ltd | Manufacturing | 2015 |
| Hogarth Plant Operator Ltd | Construction | 2014 |
| House of Gaia Ltd | Wholesale & retail | 2020 |
| House of Sika Limited | Wholesale & retail | 2024 |
| HPL Consulting Ltd | Information & communication | 2013 |
| Hyndford DEV Co Limited | Construction | 2017 |
| I R Commercial Property Company Limited | Real estate | 2017 |