| Dolphin Decorators Limited | Construction | 2003 |
| Domeo Ltd | Wholesale & retail | 2011 |
| Dominic Kirkbride Ltd | Professional & scientific | 2025 |
| Dominic Swift Limited | Other services | 2014 |
| DON Fraser Coaches Ltd | Transport & logistics | 2020 |
| Dorrian Electrical Contractors Limited | Construction | 2000 |
| Doublehelixconsulting Ltd | Professional & scientific | 2026 |
| Dowd Assets Ltd | Real estate | 2025 |
| Dowling Letting Ltd | Real estate | 2023 |
| Dowls M&E Ltd | Energy | 2022 |
| Downholland Recovery Limited | Transport & logistics | 2015 |
| DR Charlotte Ltd | Health & social care | 2012 |
| Dreamtastic Ltd | Health & social care | 2009 |
| DRG Hairdressing Limited | Other services | 2026 |
| Drivebox.App Ltd | Information & communication | 2022 |
| Drivestyledriveways Limited | Construction | 2024 |
| DS Pugh Ltd | Real estate | 2023 |
| DS Renovations Ltd | Construction | 2025 |
| DS Wraps Ltd | Construction | 2018 |
| DSE Facilities Management Ltd | Other services | 2023 |
| Dstc Holdings Ltd | Real estate | 2021 |
| DT Logistics Group Ltd | Transport & logistics | 2026 |
| DTG Services Limited | Transport & logistics | 2020 |
| DTS Fabrications Limited | Manufacturing | 2011 |
| DTS Merseyside Ltd | Transport & logistics | 2017 |
| DU Renewable Energy Service Ltd | Professional & scientific | 2025 |
| Duffy Moore Holdings Limited | Professional & scientific | 2003 |
| Duke Drainage Solutions Ltd | Water & waste | 2026 |
| Duncan Ross Land Drainage Limited | Construction | 1991 |
| Durham Group Limited | Hospitality | 2017 |
| DW Coaching Group Ltd | Arts & recreation | 2025 |
| Dynamic Landscapes Ltd | Administrative & support | 2026 |
| E-Cubed Communications Ltd | Education | 2006 |
| E4T Transport Consultants Ltd | Professional & scientific | 2019 |
| EA Formby (NW) Limited | Agriculture & fishing | 2017 |
| Easicampers Limited | Administrative & support | 2022 |
| Easm Cleans Ltd | Wholesale & retail | 2026 |
| Eastern Neonatal Ltd | Professional & scientific | 2026 |
| Eastgate Cleans Ltd | Administrative & support | 2025 |
| Easy Cocktail Limited | Wholesale & retail | 2025 |
| Easy Leisure Parks Limited | Hospitality | 2012 |
| EASYA2B Limited | Information & communication | 2000 |
| Easyatob Limited | Information & communication | 2000 |
| Ebar Limited | Hospitality | 2023 |
| EC Assets Limited | Administrative & support | 2025 |
| EC Services (2023) Ltd | Information & communication | 2023 |
| Eckersley-Bell Ltd | Professional & scientific | 2023 |
| Eco Assure Consultants Limited | Professional & scientific | 2025 |
| Eco Electrical & Building Services Limited | Construction | 2012 |
| Eco Fascia Ltd | Manufacturing | 2025 |
| Eco-Ware Essentials Ltd | Wholesale & retail | 2025 |
| Ecosash Limited | Construction | 2010 |
| Edenwhittle Limited | Professional & scientific | 2016 |
| Edge Hill Maintenance Services Limited | Administrative & support | 1997 |
| Edge Hill Students' Union Limited | Wholesale & retail | 2010 |
| Edge Hill Students' Union Trading Limited | Wholesale & retail | 2013 |
| Edge Light Ltd | Information & communication | 2025 |
| EDW Consultants Ltd | Construction | 2025 |
| EDW Trading Ltd | Construction | 2025 |
| Edward Jackson Limited | Administrative & support | 2014 |
| Edward James Stylists Ltd | Other services | 2018 |
| Edwards CNC Services Limited | Manufacturing | 2015 |
| EGG Box Properties Ltd | Real estate | 2022 |
| Egor Business Ltd | Professional & scientific | 2026 |
| Eights Food Services Limited | Hospitality | 2021 |
| Eilanne Limited | Construction | 2014 |
| EJB Building Services Ltd | Construction | 2021 |
| Elara Skin & Aesthetics Ltd | Other services | 2026 |
| Electrical Design & Projects Limited | Professional & scientific | 2014 |
| Elementary (Ormskirk) Ltd | Other services | 2015 |
| ELF Forest Limited | Arts & recreation | 2025 |
| Elite Barbering Ltd | Other services | 2018 |
| Elizabeth RAY Ltd | Wholesale & retail | 2020 |
| Elizabeth Thomson Property Developments Limited | Construction | 2021 |
| Elizabeth Training Limited | Professional & scientific | 2013 |
| Ellison Commercial Services Ltd | Wholesale & retail | 2025 |
| Ellison-Adams Ltd | Administrative & support | 2014 |
| Elwood Estates Limited | Construction | 2013 |
| Elysian Bathrooms & Kitchens Ltd | Construction | 2018 |
| Emergency Medical Transport Ltd | Other services | 2018 |
| Emery Excavation and Plant Hire Limited | Construction | 1996 |
| Emitas Ltd | Construction | 2012 |
| Empaas Ltd | Information & communication | 2019 |
| Empathy Group Limited | Administrative & support | 2024 |
| Empire Bespoke Gates Limited | Construction | 2025 |
| Empire Canal Boats Limited | Professional & scientific | 2025 |
| Empire Lets & Management Ltd | Households | 2019 |
| Empower Solutions Limited | Professional & scientific | 2024 |
| Encompass Universal Limited | Transport & logistics | 2000 |
| Endeavour Facilities Management Ltd | Real estate | 2022 |
| Endeavour Fire Safety Services Limited | Professional & scientific | 2021 |
| Endeavour Safety Services Ltd | Professional & scientific | 2018 |
| Energy Advice Helpline Community Interest Company | Other services | 2023 |
| Engage Labs Ltd | Information & communication | 2024 |
| Engineering Process Improvement Consultants Limited | Administrative & support | 2014 |
| English Speaking Board (International) Limited | Education | 1976 |
| Enhancements by Charlotte Ltd | Other services | 2025 |
| Enki Barn Ltd | Real estate | 2024 |
| Enterprise Brands Ltd | Wholesale & retail | 2003 |
| Enviro Tree Services Limited | Agriculture & fishing | 2020 |