| Sorica Transport Ltd | Transport & logistics | 2025 |
| Soteria Healthcare Ltd | Health & social care | 2022 |
| Souma Zadah Limited | Wholesale & retail | 2018 |
| Sound Damped Steel Ltd | Manufacturing | 2015 |
| South Down Signs Ltd | Manufacturing | 2016 |
| Southlands Estates Ltd | Construction | 2019 |
| Spare Electric Limited | | 1967 |
| Sparestraders Ltd | Construction | 2009 |
| Sparkle CMS Ltd | Administrative & support | 2019 |
| Sparky Clean Valets Ltd | Administrative & support | 2026 |
| Special Connections CIC | Health & social care | 2023 |
| Specialised Electronic Services Ltd | Manufacturing | 2002 |
| Specialist LOG Cabins Limited | Manufacturing | 2014 |
| Spectrum Signs & Graphics Ltd | Professional & scientific | 2024 |
| Spedbridge Global Ltd | Transport & logistics | 2021 |
| Speedy Fleet Ltd | Transport & logistics | 2023 |
| Spencroft Ceramics Limited | Manufacturing | 1976 |
| Sphere Digital Services Ltd | Information & communication | 2023 |
| Spice Box Catering Limited | Hospitality | 2019 |
| Spik N Span Cleaners Limited | Administrative & support | 2018 |
| Spine Align and Recovery Ltd | Health & social care | 2025 |
| Spiritually Guide ME Ltd | Other services | 2026 |
| Splam Ltd | Real estate | 2025 |
| Spokes Bikeshop Ltd | Wholesale & retail | 2016 |
| Sponsorship Services Ltd | Professional & scientific | 2019 |
| Sportssage Limited | Health & social care | 2002 |
| Spray Vision North East Ltd | Construction | 2025 |
| Spredhadar Ltd | Water & waste | 2025 |
| Springfield Auto Enhancement Ltd | Wholesale & retail | 2025 |
| Sprint Cargo Ltd | Transport & logistics | 2015 |
| Sprocket Kids Club C.I.C. | Education | 2015 |
| SPY Options Pick Limited | Education | 2023 |
| Spydertech UK Ltd | Information & communication | 2022 |
| SQS Holdings Limited | Real estate | 2025 |
| Squad Developments Limited | Real estate | 2024 |
| Squad Labs Ltd | Information & communication | 2024 |
| SR Car & Commercials Limited | Wholesale & retail | 2015 |
| SR Software Consultant Limited | Information & communication | 2018 |
| SRI Lakshmi Travels Ltd | Transport & logistics | 2022 |
| Srishtav Ltd | Information & communication | 2026 |
| SRP Solutions Ltd | Professional & scientific | 2014 |
| SS Groundworks North East Limited | Construction | 2024 |
| SS Medical Consultancy Ltd | Professional & scientific | 2018 |
| SS Renewables Ltd | Energy | 2024 |
| SSG Motors Stoke Ltd | Wholesale & retail | 2025 |
| Sssb Limited | Health & social care | 2020 |
| SSY Properties Limited | Real estate | 2024 |
| ST Mary's Healthcare Limited | Health & social care | 2021 |
| ST Mary's Local Stores Limited | Wholesale & retail | 2016 |
| ST Property Holdings Limited | Extraterritorial | 2021 |
| ST. Paul's Court Blurton Management Company Limited | Households | 1981 |
| ST. Quentin Residential Home Limited | Health & social care | 1988 |
| ST5 Properties Ltd | Real estate | 2025 |
| ST5LOGISTICS Limited | Transport & logistics | 2025 |
| Stafford Breakdown & 24HR Mobile Tyre Services Ltd | Wholesale & retail | 2020 |
| Stafford South Limited | Administrative & support | 2018 |
| Staffordshire Aerials Ltd | Information & communication | 2022 |
| Staffordshire Black Business Network Limited | Professional & scientific | 2024 |
| Staffordshire Care Direct Limited | Health & social care | 2017 |
| Staffordshire Classic Scooters Ltd | Wholesale & retail | 2023 |
| Staffordshire Cleaning & Maintenance Limited | Administrative & support | 2014 |
| Staffordshire Kitchens and Bedrooms Limited | Wholesale & retail | 1995 |
| Staffordshire Lettings Ltd | Real estate | 2020 |
| Staffordshire Precision Engineering Limited | Manufacturing | 1981 |
| Staffordshire Savoury Scotch Eggs Limited | Manufacturing | 2016 |
| Staffordshire Tuition Limited | Education | 2010 |
| Staffs Civils & Surfacing Contractors Limited | Construction | 2023 |
| Staffs Industrial Doors Limited | Manufacturing | 2004 |
| Staffs Motorcycles Limited | Wholesale & retail | 2020 |
| Staffs Property Services (NO2) Ltd | Construction | 2021 |
| Staffs Property Services Ltd | Real estate | 2015 |
| Staffs Property Solutions Ltd | Real estate | 2020 |
| Staffs Vehicle Hire and Repairs Limited | Administrative & support | 2020 |
| Staffs-Cctv Limited | Construction | 2006 |
| Staffy Investments Ltd | Real estate | 2020 |
| Stairlifts in Staffordshire Ltd | Extraterritorial | 2010 |
| Stam Holdings Limited | Construction | 1995 |
| Stand OUT Nation Ltd | Wholesale & retail | 2022 |
| Standing in the MAP Ltd | Administrative & support | 2021 |
| Stanton Estates Limited | Construction | 2019 |
| Starbajan GP Ltd | Education | 2019 |
| Stars Smile Limited | Health & social care | 2012 |
| Stars Smile Properties Limited | Real estate | 2017 |
| Staw Ltd. | Wholesale & retail | 2004 |
| Stay in the Black Ltd | Professional & scientific | 2011 |
| Stayindependant Ltd | Health & social care | 2025 |
| Steeloak Group Ltd | Professional & scientific | 2024 |
| Steven Rowell (Electrics) Ltd | Construction | 2015 |
| Stileglass Ltd | Manufacturing | 2023 |
| Stock Pro Capital Limited | Financial services | 2025 |
| Stockifyuk Ltd | Professional & scientific | 2022 |
| Stoke & Staffordshire Equity Partners Limited | Administrative & support | 2020 |
| Stoke Blaster's Cricket Club Ltd | Arts & recreation | 2026 |
| Stoke Electrical Limited | Wholesale & retail | 2010 |
| Stoke Luxury Travels Ltd | Transport & logistics | 2019 |
| Stoke Mini Works Ltd | Wholesale & retail | 2022 |
| Stoke on Trent Hair Loss Clinic Ltd | Other services | 2023 |
| Stoke PC Supplies Ltd | Wholesale & retail | 2026 |
| Stoke Youth Musical Theatre Company | Arts & recreation | 2011 |
| Stoked Studios Limited | Professional & scientific | 2024 |