| Bioclough Consultants Limited | Professional & scientific | 2015 |
| Birch Strategic Services Ltd | Professional & scientific | 2024 |
| Birchover Reclamation Ltd | Wholesale & retail | 2019 |
| Birchover Sales and Lettings Limited | Real estate | 2015 |
| Birmingham Precision Engineering Limited | Manufacturing | 2012 |
| Biross Design Limited | Administrative & support | 2008 |
| Black Cat Bars Ltd | Hospitality | 2011 |
| Black Feet Studios Ltd | Arts & recreation | 2026 |
| Blades of Gray Limited | Other services | 2021 |
| Blaenavon Forgings Limited | Manufacturing | 2019 |
| Blaze Hospitality UK Ltd | Hospitality | 2023 |
| Blood Estate Ltd | Real estate | 2021 |
| Bloom Bars NUL Ltd | Hospitality | 2020 |
| Blucon Demolition Limited | Construction | 2015 |
| Blue Water Clothing Ltd. | Wholesale & retail | 2004 |
| Blueberry Cafe (Castleton) Ltd | Hospitality | 2017 |
| Blueberry Cafe Limited | Hospitality | 2013 |
| Blunt Crayon Ltd | Professional & scientific | 2018 |
| Bmorecreative Limited | Administrative & support | 2006 |
| BNG Consulting Limited | Professional & scientific | 2022 |
| Bobby Dog Ltd | Health & social care | 2021 |
| Bond Capital Partners (UK) Limited | Financial services | 2005 |
| Bond Capital Partners Limited | Financial services | 2005 |
| Boomwriter Ltd | Information & communication | 2024 |
| Bora Unlimited Ltd | Hospitality | 2023 |
| Boss Metal Roofing Ltd | Construction | 2000 |
| Boston Brewery Ltd | Manufacturing | 2024 |
| BOW Boutique Ltd | Wholesale & retail | 2012 |
| Boxset Media Ltd | Information & communication | 2010 |
| Brabourne Limited | Information & communication | 2011 |
| Bradmans Ltd | Hospitality | 2020 |
| Brassington Community Heating CIC | Construction | 2021 |
| Brassington Honey Limited | Manufacturing | 2024 |
| Breakthrough Bars Ltd | Hospitality | 2023 |
| Bretti UN Limited | Financial services | 2024 |
| Briant Property Holdings Ltd | Hospitality | 2019 |
| Briar Consulting Limited | Professional & scientific | 2013 |
| Briars Inn Lettings Limited | Real estate | 2023 |
| Bricks and Mortar (Wirksworth) Ltd | Real estate | 2019 |
| Briddon Holdings Limited | Financial services | 2023 |
| Briddon Homes Limited | Construction | 2000 |
| Bridgecraft Engineering Limited | Professional & scientific | 2002 |
| British Orienteering Federation Limited | Arts & recreation | 1982 |
| BRJ Support Services Ltd | Health & social care | 2019 |
| Broadband Infrastructure Company Ltd | Information & communication | 2017 |
| Broccoli & Bees Limited | Wholesale & retail | 2025 |
| Brooks Cordon Consulting Limited | Professional & scientific | 2015 |
| Brooks Engineering Limited | Manufacturing | 2020 |
| Broomfield Consultants Limited | Information & communication | 1996 |
| Brosterfield Engineering Services Limited | Manufacturing | 2000 |
| BSP Holdings Developments Limited | Real estate | 2021 |
| BSP Holdings Management Limited | Real estate | 2021 |
| BSP Holdings Nottingham Limited | Real estate | 2021 |
| BSP Property Management Limited | Real estate | 2021 |
| Budget Self Storage Limited | Other services | 2010 |
| Budget Tyres (Wirksworth) Ltd. | Wholesale & retail | 2002 |
| Build by Milestone Ltd | Construction | 2021 |
| Build by Milestone Matlock Ltd | Construction | 2026 |
| Burrows & Hare Esquires Limited | Wholesale & retail | 2022 |
| Burton Environmental Services Limited | Water & waste | 2005 |
| Bushwood Venture Ltd | Real estate | 2013 |
| Business Consultants (Derbyshire) Limited | Real estate | 1955 |
| Butlers Arundel Limited | Hospitality | 2017 |
| Button Training Ltd | Wholesale & retail | 2019 |
| C H Recycling Solutions Ltd | Water & waste | 2019 |
| C. Farmer Limited | Real estate | 1957 |
| C.G. Landscapes Limited | Administrative & support | 2004 |
| C.N.C. Manufacturing Limited | Manufacturing | 1997 |
| C.R.T. Limited | Administrative & support | 2002 |
| Cactus Tree Guards Limited | Wholesale & retail | 2021 |
| Cakeshake Desserts Ltd | Hospitality | 2025 |
| Calvert Cars Ltd | Wholesale & retail | 2025 |
| Cambrian Business Improvement Limited | Administrative & support | 2016 |
| Cameron Gill Ltd | Transport & logistics | 2021 |
| Camerons Northwood Limited | Professional & scientific | 2004 |
| Camfordbridge Ltd | Education | 2019 |
| Camper Escapes Ltd | Manufacturing | 2022 |
| Cantonese WOK Ltd | Hospitality | 2023 |
| Capstone Consulting Engineers Limited | Professional & scientific | 2003 |
| Cardale Food Limited | Hospitality | 2024 |
| Cardboard Box Theatre Company CIC | Arts & recreation | 2026 |
| Careport Advisory Services (Holdings) Limited | Health & social care | 2014 |
| Careport Advisory Services Limited | Health & social care | 2009 |
| Carl Banton Enterprises Limited | Wholesale & retail | 2019 |
| Carl Taylor Building and Roofing Ltd | Extraterritorial | 2021 |
| Carnall Brothers Ltd | Real estate | 2026 |
| Carrieri North Limited | Hospitality | 2021 |
| Carroliann Ltd | Hospitality | 2019 |
| Carrotcruncher Media Ltd | Information & communication | 2020 |
| Carsington Property Company Limited | Real estate | 2013 |
| Carson Plant Hire Limited | Administrative & support | 2024 |
| Cartlidge Pump Solutions Ltd | Professional & scientific | 2023 |
| Castle Fifteen Consulting Ltd | Water & waste | 2020 |
| Castle Fuels Limited | Wholesale & retail | 2020 |
| Castle Green Matlock Limited | Hospitality | 2012 |
| Catalytiq Ltd | Information & communication | 2025 |
| Catherine Fraser Ltd | Health & social care | 2023 |
| Caudwell (Rowsley) Farms Limited | Agriculture & fishing | 1965 |
| Caudwell CR Limited | Professional & scientific | 2016 |
| Caudwell Flour Ltd | Wholesale & retail | 2023 |