| Arlington Building Projects Limited | Construction | 2007 |
| Arlington GP Limited | Financial services | 2018 |
| ARM(2) Law Limited | Professional & scientific | 2018 |
| Aroma Lounge Matlock Ltd | Hospitality | 2024 |
| Artaine Limited | Agriculture & fishing | 2007 |
| Arthur Cottam & Co.(Horse Shoes)Limited | Manufacturing | 1963 |
| Artifice Industries Limited | Information & communication | 2016 |
| Artistry Defined Ltd | Other services | 2023 |
| Ascent Tree Solutions Ltd | Administrative & support | 2022 |
| Ashbourne Engineering Limited | Manufacturing | 1999 |
| Ashbrook Commercial Properties Limited | Real estate | 2021 |
| Ashbrook Construction (Matlock) Limited | Construction | 2015 |
| Ashbrook Holding Group Limited | Financial services | 2021 |
| Ashbrook Roofing Services Limited | Construction | 2022 |
| Ashford Mill Developments Ltd | Energy | 2008 |
| Ashlyne Fabrications Limited | Manufacturing | 2000 |
| Ashover Business Centre Limited | Manufacturing | 2008 |
| Ashover Investment Management Ltd | Extraterritorial | 2014 |
| Ashton & Coleman Limited | Manufacturing | 2019 |
| Ashtton Associates Ltd | Professional & scientific | 2005 |
| Ashworth Car Centre Ltd | Wholesale & retail | 2023 |
| Aspire Creative Hair Limited | Other services | 2021 |
| Assured FG Ltd | Financial services | 2020 |
| Assured Safety & Health Limited | Health & social care | 2014 |
| Astbury & Taylor (Motor Engineers) Limited | Wholesale & retail | 2002 |
| Astute Accountancy Group Limited | Professional & scientific | 2024 |
| Atoh Limited | Real estate | 2016 |
| Attachment Works Limited | Other services | 2009 |
| Aurelien Ltd | Other services | 2012 |
| AUTH365 Ltd | Information & communication | 2025 |
| Automotivate Vehicle Hire and Leasing Limited | Wholesale & retail | 2022 |
| Automotivation Solutions Limited | Administrative & support | 2022 |
| Autotek Body Repairs Ltd | Wholesale & retail | 2011 |
| Avenue Digital Limited | Professional & scientific | 2015 |
| Avli Construction Services Limited | Professional & scientific | 2023 |
| Avochie Granite Co. Limited | Extraterritorial | 1996 |
| Axi-Dent (Northants) Ltd | Wholesale & retail | 2007 |
| Axis Partners Ltd | Information & communication | 2026 |
| Aycee Products & Services Ltd | Construction | 2024 |
| B&J Consultancy Ltd | Professional & scientific | 2020 |
| B.J.Plastics(Wolverhampton)Limited | Manufacturing | 1971 |
| B.J.Waters (Transport) Limited | Transport & logistics | 1961 |
| Backchat Productions Ltd | Information & communication | 2022 |
| Bailey's Mill Management Company Limited | Households | 2003 |
| Baileycroft Mews Management Company Limited | Households | 2019 |
| Baker Consultants Limited | Professional & scientific | 2008 |
| Bakewell Reclaimed Stone Limited | Mining & quarrying | 2015 |
| Bakewell Windows Limited | Construction | 1993 |
| Balbin Limited | Real estate | 2003 |
| Bamford and Leigh Ltd | Construction | 2025 |
| Bamford Planning Limited | Professional & scientific | 2026 |
| Bank Gardens Flat Management Company Limited | Households | 2009 |
| Bank Manor Freehold Limited | Hospitality | 2015 |
| Bannister's Barbers Limited | Other services | 2018 |
| Bannister's Properties Limited | Real estate | 2018 |
| Barn Farm Storage Limited | Other services | 2010 |
| Barnett Development and Lets Limited | Construction | 2023 |
| Barnsdall Electrical Services Limited | Construction | 2010 |
| Barra Point Advisory Ltd | Professional & scientific | 2025 |
| Barracuda Digital Limited | Professional & scientific | 2000 |
| Barry Wood Building Contractor Limited | Construction | 2009 |
| Barton Hall Developments Limited | Real estate | 2019 |
| Bartreyee Property Limited | Financial services | 2022 |
| Basecamp Financial Planning Limited | Financial services | 2022 |
| Bay Tree Therapeutic Play and Self-Healing Ltd | Health & social care | 2023 |
| BCB Chesterfield Ltd | Hospitality | 2013 |
| Beach Haven Caravan Park Limited | Real estate | 2021 |
| Beaumont Farming and Forestry LLP | | 2023 |
| Beauvamp Limited | Manufacturing | 2020 |
| Bedrock Bike Fit Ltd | Wholesale & retail | 2024 |
| Beechdale (Matlock) Limited | Manufacturing | 1998 |
| Beedham Antiques Limited | Wholesale & retail | 1981 |
| Beeplanned Limited | Professional & scientific | 2015 |
| Bela Structures Limited | Construction | 2016 |
| Belle Care Services Ltd | Professional & scientific | 2011 |
| Bellrose Care Ltd | Professional & scientific | 2007 |
| Bellwether Holdings Ltd | Hospitality | 2025 |
| Belper Autotech Ltd | Wholesale & retail | 2002 |
| Belper Chiropractic Clinic Limited | Health & social care | 2019 |
| Belper Hydro Limited | Energy | 2025 |
| Beltie Property Limited | Real estate | 2025 |
| Bennett Company Holdings Limited | Financial services | 2024 |
| Bentley Brook Brewery Taproom Ltd | Hospitality | 2023 |
| Bentley Brook Conservation Village (NO.1) Limited | Other services | 2001 |
| Bentley Cottages Ltd | Hospitality | 2024 |
| Bernie's Place C.I.C. | Health & social care | 2024 |
| Berri Homes Ltd | Real estate | 2018 |
| Berri Limited | Information & communication | 2011 |
| BET Estates Limited | Real estate | 2022 |
| BET Sourcing Ltd | Administrative & support | 2023 |
| BET Stays Ltd | Real estate | 2023 |
| BET Transport Ltd | Transport & logistics | 2023 |
| Better Inspiration Ltd | Health & social care | 2023 |
| Beverley Property Limited | Real estate | 2023 |
| Beyond Venture LAB Limited | Professional & scientific | 2025 |
| Bibi Pictures Ltd | Information & communication | 2026 |
| Biddlecombe Properties Limited | Real estate | 2022 |
| Bike Fit KIT Ltd | Wholesale & retail | 2022 |
| Bilberry Breaks Limited | Real estate | 2026 |
| Binman Games Limited | Information & communication | 2022 |