| SJC Contracting Services Limited | Information & communication | 2018 |
| SJG Global Consulting Ltd | Professional & scientific | 2025 |
| SKA Real Estate Investments UK Ltd | Real estate | 2014 |
| Skelligs Commercial Limited | Construction | 2023 |
| Skelligs Renovations and Rentals Limited | Construction | 2023 |
| Skelligs Serviced Accommodation Limited | Real estate | 2023 |
| Skillsmatch Limited | Professional & scientific | 2008 |
| Skin Smiles Success Ltd | Health & social care | 2018 |
| Skippy Industries Ltd | Water & waste | 2014 |
| Skopa Ltd | Wholesale & retail | 2022 |
| Slack's Property Ltd | Construction | 2019 |
| Slater's Trading Ltd | Agriculture & fishing | 2015 |
| Slaters' (Plastikard) Limited | Manufacturing | 1972 |
| Sleekmade Property Developments Limited | Extraterritorial | 2021 |
| Sleekmade Property Management Ltd | Real estate | 2021 |
| Sleekmade Prpperty Management Ltd | Real estate | 2021 |
| Slinter Holdings Limited | Financial services | 2019 |
| Slinter Mccabe Holdings Ltd | Financial services | 2019 |
| Slinter Mining Company Limited | Mining & quarrying | 1946 |
| SM4D Ltd | Professional & scientific | 2024 |
| Smart LET (Derbyshire) Limited | Real estate | 2005 |
| Smart Living Construction and Developments Limited | Construction | 2024 |
| Smiths and Sons Building and Conservation Ltd | Construction | 2022 |
| SMS Fitness Ltd | Education | 2024 |
| SNX 503 Limited | Extraterritorial | 2017 |
| Soil Acoustics Ltd | Professional & scientific | 2024 |
| Solarice Limited | Other services | 2023 |
| Solarwatt Technologies Limited | Manufacturing | 2021 |
| Sole Interference Ltd | Professional & scientific | 2018 |
| Sorted Entertainment Ltd | Administrative & support | 2004 |
| Soulfire QI Gong Ltd | Health & social care | 2019 |
| Sourdough Dave Ltd | Manufacturing | 2024 |
| Sparkstack Marketing Limited | Information & communication | 2025 |
| Spatial Circumstances Ltd | Information & communication | 2021 |
| Specialised Sideloader Products Limited | Manufacturing | 2003 |
| Speedexpand Limited | Wholesale & retail | 1992 |
| Speeds (Burton) Limited | Real estate | 2014 |
| Speeds Court Ltd | Real estate | 2023 |
| Speeds Limited | Real estate | 1973 |
| Speeds Slaley Ltd | Real estate | 2023 |
| Spencer Bros. (1903) Brassington Limited | Manufacturing | 2008 |
| Spencer Bros. (1903) Limited | Manufacturing | 1986 |
| Spencer's Trading & Investment Group Ltd | Real estate | 2020 |
| Spice Restaurant Balti Ltd | Hospitality | 2025 |
| Spitfire Studio Projects Ltd | Professional & scientific | 2021 |
| SPK Engineering Limited | Water & waste | 2013 |
| Sportrack Synthetic Surfaces Limited | Administrative & support | 2016 |
| Square Two Development Limited | Information & communication | 2008 |
| Squarechilli Limited | Information & communication | 2012 |
| SSV Matlock Limited | Other services | 2022 |
| Stacey Asphalt Limited | Other services | 2013 |
| Stacey Crushing & Plant Hire Limited | Mining & quarrying | 2023 |
| Stacey Limited | Professional & scientific | 2021 |
| Stacey Processing Ltd | Other services | 2002 |
| Stacey Transport Limited | Transport & logistics | 2021 |
| Stack UP Storage Limited | Wholesale & retail | 2025 |
| Stainsborough Hall Limited | Hospitality | 2017 |
| Stanton Park Farm Limited | Agriculture & fishing | 1947 |
| Steadfast Commerce Ltd | Wholesale & retail | 2026 |
| Steeple Grange Light Railway Company Limited | Transport & logistics | 1987 |
| Step Forward (Nottingham) Limited | Health & social care | 1999 |
| Step Forward Psychology Ltd | Education | 2022 |
| Stephen James Technologies Limited | Manufacturing | 2000 |
| Stevie Davies Glass Limited | Wholesale & retail | 2024 |
| Stevie Software Ltd | Information & communication | 2024 |
| STL Property Group Ltd | Real estate | 2026 |
| Stokvel Limited | Other services | 2006 |
| Stone Art Limited | Wholesale & retail | 2005 |
| Stone Block Limited | Manufacturing | 2005 |
| Stone Construction Ltd. | Construction | 1993 |
| Stone Heritage Sales Limited | Manufacturing | 1993 |
| Stone Machinery Services Limited | Manufacturing | 2012 |
| Stone Oak Investments Ltd | Professional & scientific | 2013 |
| Stone Shotblasting Limited | Construction | 2023 |
| Stonebuild Building & Walling Limited | Construction | 2013 |
| Stop Fearing Fear Services Ltd | Professional & scientific | 2026 |
| Storepoint Storage Limited | Transport & logistics | 2020 |
| Stps Property Services Ltd | Real estate | 2024 |
| Straight UP Development Ltd | Real estate | 2017 |
| Strange Haulage Limited | Transport & logistics | 2015 |
| Strutt Grabs Limited | Administrative & support | 2015 |
| Studio BIO Ltd | Professional & scientific | 2020 |
| Studio Oikos Limited | Professional & scientific | 2026 |
| Studio WH Limited | Arts & recreation | 2016 |
| Sumner Hale Properties Limited | Hospitality | 2023 |
| Sunnyside Studio Ltd | Other services | 2015 |
| Superlink Healthcare Ltd | Health & social care | 2015 |
| Supplement MAD Ltd | Administrative & support | 2019 |
| Supporting Solutions Limited | Health & social care | 2007 |
| Supreme Power Ltd | Manufacturing | 2016 |
| Surefast Bolting Services Ltd | Professional & scientific | 2016 |
| Surety Care Limited | Health & social care | 2021 |
| Sutton Arms (Scawby) Ltd | Hospitality | 2012 |
| Swan Matlock Ltd | Health & social care | 2024 |
| Swanston Publishing Limited | Information & communication | 1983 |
| Swift Peak Security Systems Ltd | Wholesale & retail | 2026 |
| Swift Tyre Repairs Limited | Wholesale & retail | 1996 |
| Switchforbusiness Ltd | Administrative & support | 2013 |
| SWR Property Holdings Limited | Real estate | 2020 |
| Sycamore Pet & Equestrian Limited | Wholesale & retail | 2020 |