| Meadows Site Services Limited | Water & waste | 2020 |
| Mearns and Heddle Stonemasons and Specialist Builders Ltd | Construction | 2021 |
| Merchants House Cafe Ltd | Manufacturing | 2020 |
| Michael FOX Roofing Limited | Construction | 2025 |
| Mindful Monkeys Ltd | Education | 2017 |
| MK Properties (Scotland) Limited | Real estate | 2022 |
| MMB Drywall Services Ltd | Construction | 2018 |
| Mobile Tower Solutions Limited | Information & communication | 2018 |
| Modern Home Products Limited | Wholesale & retail | 2019 |
| Moret Landscaping Limited | Administrative & support | 2022 |
| Motorsport With Attitude Limited | Administrative & support | 2023 |
| MPH Home Ltd | Construction | 2015 |
| MR Vlad Ltd | Construction | 2017 |
| MRV Group Ltd | Construction | 2021 |
| MS365 Future Ltd | Information & communication | 2025 |
| MT Utility Ltd | Construction | 2025 |
| MTG Enterprises Ltd | Transport & logistics | 2023 |
| MW Vehicle Servicing & Repairs Ltd | Wholesale & retail | 2025 |
| Nisa Local Cardenden Limited | Wholesale & retail | 2025 |
| Nor-Tech IT Solutions Ltd | Information & communication | 2017 |
| NRR Foods Ltd | Hospitality | 2026 |
| Orbital Tech Ltd | Information & communication | 2018 |
| ORE Valley Community Initiatives Ltd. | Professional & scientific | 2003 |
| ORE Valley Energy Limited | Energy | 2016 |
| ORE Valley Heating Limited | Construction | 2016 |
| Orebank Plumbing & Heating Ltd | Construction | 2014 |
| Osborne & Allan Ltd. | Administrative & support | 2017 |
| Owen Buchanan Builders Limited | Construction | 2002 |
| Paterson Joinery 1 Ltd | Construction | 2024 |
| Paterson Property Maintenance Ltd | Construction | 2016 |
| Pauly & Co Limited | Real estate | 2009 |
| Pawesome Parties Ltd | Other services | 2025 |
| Personalise ME (Scotland) Ltd | Wholesale & retail | 2020 |
| PG Fife Limited | Hospitality | 2023 |
| PH Properties Scotland Ltd Limited | Real estate | 2024 |
| Phoenix Finishing Solutions Ltd | Construction | 2023 |
| Phoenix Scotia Limited | Information & communication | 2019 |
| Pink Ballerina Ltd | Information & communication | 2012 |
| Piperight Plumbing & Heating Limited | Construction | 2025 |
| Powerplay Media Limited | Information & communication | 2023 |
| Ppas Scotland Ltd | Administrative & support | 2021 |
| Presto Projects Ltd | Information & communication | 2021 |
| Preta Properties Limited | Construction | 2020 |
| Prints Charming Fife Limited | Manufacturing | 2021 |
| Prints Charming Holdings Ltd | Financial services | 2024 |
| Pro Maintenance (Fife) Ltd | Administrative & support | 2016 |
| Pro-Duct Fabrications Limited | Manufacturing | 2014 |
| Purvis Storage & Distribution Ltd | Transport & logistics | 2013 |
| PV Gas&Heating Ltd | Construction | 2018 |
| Qazim Limited | Professional & scientific | 2022 |
| Qazim Properties Limited | Real estate | 2024 |
| QMI Properties Limited | Real estate | 2018 |
| R.M. Kitchens Limited | Construction | 2023 |
| Realm Construction Limited | Construction | 1988 |
| Recycle Fife Limited | Water & waste | 2021 |
| Red Seal Concrete Forms Ltd | Manufacturing | 2024 |
| Reem Valet Ltd | Extraterritorial | 2025 |
| Regal Rubber Company Ltd. | Manufacturing | 2013 |
| Regal Rubber Partnership LLP | | 2010 |
| Rely Motors Ltd | Wholesale & retail | 2019 |
| Remus Equestrian Centre Ltd | Arts & recreation | 2010 |
| Renroc Hill Ltd | Administrative & support | 2016 |
| Restoration Fife Ltd | Health & social care | 2020 |
| Revibel Limited | Information & communication | 2024 |
| Revued Ltd | Information & communication | 2023 |
| Reynolds Building and Landscaping Ltd | Construction | 2026 |
| RHI Limited | Construction | 2009 |
| RJA Tech Services Ltd | Construction | 2022 |
| RJE Access Solutions Limited | Professional & scientific | 2022 |
| RMC Property Holdings (Fife) Ltd | Financial services | 2024 |
| Rmparcels Ltd | Transport & logistics | 2026 |
| Robert Bailey Bespoke Ltd | Manufacturing | 2020 |
| Robert Purvis Plant Hire Limited | Administrative & support | 1980 |
| Rocky Lettings Limited | Real estate | 2024 |
| Romulus Land Investments Limited | Real estate | 2018 |
| Rose Sales Retail Ltd | Wholesale & retail | 2021 |
| RRL Properties Ltd | Real estate | 2025 |
| RSK Property Maintenance Ltd | Construction | 2025 |
| RV Woodcraft Limited | Manufacturing | 2024 |
| Ryan Doyle Brickwork Ltd | Construction | 2023 |
| Ryan O'Shea Ltd | Professional & scientific | 2026 |
| S & R Transport Limited | Professional & scientific | 2009 |
| S Carrick Ltd. | Health & social care | 2009 |
| S H Bar Ltd | Hospitality | 2025 |
| S Smith Bricklaying Limited | Construction | 2019 |
| S.D.A (Fife) Limited | Construction | 2025 |
| Sala-Murray Roofing Limited | Construction | 2015 |
| Samuel’S Driving Limited | Transport & logistics | 2025 |
| Sandy Cochrane Builders Limited | Construction | 2014 |
| Scott Storage Containers (Glenrothes) Ltd | Other services | 2017 |
| Scruff to Fluff Grooming by Caitlin Limited | Other services | 2025 |
| Seath Holdings Ltd | Financial services | 2025 |
| Secureshred Limited | Professional & scientific | 2009 |
| Secureshred Scotland Limited | Water & waste | 2008 |
| Seha Locum Ltd | Public administration | 2025 |
| Self Deliveries Ltd | Transport & logistics | 2015 |
| Serenity Fife Ltd | Other services | 2016 |
| Sher Plus Limited | Wholesale & retail | 2019 |
| Shreya Food Limited | Hospitality | 2026 |
| SI Select Foods Ltd | Hospitality | 2018 |