| Grey Bedford Ltd. | Administrative & support | 2005 |
| Grow Training Ltd | Information & communication | 2013 |
| GS Properties (Fife) Ltd | Real estate | 2019 |
| Haute Decor Limited | Construction | 2025 |
| HAY and HAY Homes Ltd | Construction | 2013 |
| Hector Building Limited | Construction | 2025 |
| Hedgewood Land Limited | Real estate | 2019 |
| Henderson Haulage Ltd | Transport & logistics | 2025 |
| Herron Hire Ltd | Extraterritorial | 2021 |
| Highland Solutions Ltd | Professional & scientific | 2024 |
| Highway Electrical Lighting Consultants Limited | Professional & scientific | 2019 |
| HMF Consultancy Limited | Professional & scientific | 2022 |
| HMF Properties Limited | Real estate | 2021 |
| Horizon Fife Ltd | Construction | 2019 |
| Hutchison Dimensional Control Limited | Professional & scientific | 2013 |
| I and M Property Investments Limited | Real estate | 2018 |
| I.S Plumbing & Heating Fife Ltd | Administrative & support | 2017 |
| Ian's Coaches Ltd. | Transport & logistics | 2006 |
| Ig&Fr Ltd | Hospitality | 2022 |
| Ijgas&Plumbing Ltd | Construction | 2023 |
| Ilyas Sons Limited | Wholesale & retail | 2024 |
| Impressed Limited | Construction | 2014 |
| Industrial Gutters and Roofing Limited | Construction | 2016 |
| Inspiring Digital Ltd | Information & communication | 2025 |
| Intelligroup UK Services Ltd | Administrative & support | 2024 |
| Intersupplier Ltd | Transport & logistics | 2018 |
| Inverted Diamond Ltd | Education | 2025 |
| ITS BED and Pine Limited | Wholesale & retail | 2017 |
| IW Stonemasons Ltd | Manufacturing | 2016 |
| J & G Duggan Builders Ltd | Construction | 2017 |
| J J Walker Developments Ltd | Construction | 2025 |
| J&J Steel Build Limited | Construction | 2015 |
| J. Main Offshore Services Ltd | Construction | 2022 |
| J.A.D Rentals Ltd | Real estate | 2021 |
| Jamie Blake Property Holdings Ltd | Real estate | 2021 |
| Jamie Blake Retail Ltd | Wholesale & retail | 2024 |
| Jamie Blake Trade Ltd | Wholesale & retail | 2024 |
| Jamie Mcnee Joinery Limited | Construction | 2024 |
| JFB Solutions Ltd | Professional & scientific | 2015 |
| John a Sellars and Associates Limited | Professional & scientific | 2018 |
| John Gilfillan Funeral Directors Limited | Other services | 2016 |
| John Mclean Joiners Ltd | Manufacturing | 2017 |
| Joseph Kirby Plastering and Roughcasting Ltd | Construction | 2025 |
| JPS Joiners Ltd | Construction | 2025 |
| JPS Plumbing and Heating Ltd | Construction | 2018 |
| JS Builders (Scotland) Limited | Administrative & support | 2006 |
| JWF Joiners Limited | Construction | 2025 |
| K E Wilson Ltd | Hospitality | 2018 |
| Kaizen Martial Arts Ltd | Wholesale & retail | 2025 |
| Kaynes Holdings Ltd | Information & communication | 2019 |
| KD Renovation Ltd | Real estate | 2024 |
| KDA Construction Ltd | Construction | 2025 |
| Kestrel Hire EU Limited | Other services | 2009 |
| KF Brickworks Limited | Construction | 2015 |
| KF Lettings Limited | Real estate | 2024 |
| Kingdom Holiday Rentals Limited | Real estate | 2024 |
| Kingdom Investment Properties Ltd | Real estate | 2022 |
| Kingdom Sports Ltd | Wholesale & retail | 2019 |
| Knowehead Garage Autos Ltd | Wholesale & retail | 2023 |
| KRM Developments Fife Limited | Construction | 2025 |
| Kvuk Lochgelly Ltd | Wholesale & retail | 2023 |
| L & N Lettings Limited | Real estate | 2017 |
| L and J Bars Ltd | Hospitality | 2019 |
| L&L Keicher Properties Ltd | Real estate | 2025 |
| L.A.B Roofing & Roughcasting Limited | Construction | 2025 |
| L.A.B Roofing Services Ltd | Construction | 2026 |
| Ladyinch Equestrian Ltd | Education | 2022 |
| Ladyinch Estates Ltd | Real estate | 2022 |
| Ladyinch Group Ltd | Financial services | 2022 |
| Ladyinch Management Limited | Professional & scientific | 2015 |
| Langside Quarry Limited | Mining & quarrying | 2023 |
| Laraz Limited | Real estate | 2018 |
| Larry Burns Bricklayer Ltd | Construction | 2021 |
| Lcom GAS Limited | Construction | 2024 |
| LD Rope Access Ltd | Manufacturing | 2023 |
| LDL Enterprise Ltd. | Real estate | 2012 |
| Let's Begin Counselling Ltd | Health & social care | 2025 |
| Littlejohn Electrical Limited | Administrative & support | 2007 |
| LKG Food Ltd | Hospitality | 2025 |
| LL Ventilation Ltd | Construction | 2016 |
| Local Darts Shop Online Ltd | Wholesale & retail | 2025 |
| Loch Leven Developments Limited | Construction | 2020 |
| Lochgelly Autocentre Ltd | Wholesale & retail | 2020 |
| Lochgelly Barbers Ltd | Other services | 2023 |
| Lomond 67 Limited | Extraterritorial | 2013 |
| LSK Capital Ltd | Information & communication | 2025 |
| Lumina Partnership LLP | | 2022 |
| Lunalink Ltd | Wholesale & retail | 2026 |
| Lynda K. Aesthetics Ltd | Other services | 2021 |
| M&C Mclean Industrial & Agricultural Roofing & Cladding Ltd | Construction | 2022 |
| MAR Scaffolding (Scotland) Limited | Construction | 1992 |
| Mark Campbell Consultancy Ltd | Professional & scientific | 2017 |
| Markinch Developments Limited | Construction | 2024 |
| MBM Healthcare Fife Ltd | Wholesale & retail | 2020 |
| MBM Healthcare Wales Ltd | Wholesale & retail | 2026 |
| MBR Lettings Ltd | Real estate | 2024 |
| Mcdonald Access Solutions Ltd | Construction | 2024 |
| Mcguire Construction Consultancy Limited | Construction | 2018 |
| Mclean Building and Roofing Limited | Construction | 2020 |
| MCS Ready Ltd | Education | 2025 |