| Comprehensive Care & Support Services Limited | Health & social care | 2022 |
| Condor Motorsports Club Limited | Arts & recreation | 2018 |
| Connected Care Services Ltd | Health & social care | 2019 |
| Connor Handyman Ltd | Other services | 2023 |
| Context Labs Ltd | Wholesale & retail | 2023 |
| CQ Joinery Ltd | Construction | 2019 |
| Creator Works Ltd | Professional & scientific | 2020 |
| Crew Network Ltd | Professional & scientific | 2020 |
| Crew Services Ltd | Professional & scientific | 2020 |
| Crosshill Hand Car Wash Ltd | Administrative & support | 2023 |
| CSH Enterprises Ltd | Professional & scientific | 2024 |
| Cuileann Ltd | Wholesale & retail | 2020 |
| D & T Brown Property Limited | Real estate | 2022 |
| D Hall Renewables Ltd | Manufacturing | 2017 |
| D Thomson Bricklaying Limited | Construction | 2019 |
| D2D Lochgelly Ltd | Wholesale & retail | 2024 |
| Daniel Zygadlo Limited | Professional & scientific | 2016 |
| Danielcompany Ltd | Transport & logistics | 2020 |
| Dash Events Scotland Limited | Transport & logistics | 2021 |
| Dash Medical Services Ltd | Transport & logistics | 2023 |
| Dastaan Food Ltd | Hospitality | 2026 |
| Dave Drummond General Contractors Ltd | Construction | 2019 |
| DD Motor Services Limited | Wholesale & retail | 2026 |
| DDJ Heating and Renewables Limited | Construction | 2025 |
| DDJ Heating Engineer Ltd | Construction | 2021 |
| Declan Carberry Welding Solutions Ltd | Manufacturing | 2024 |
| Delavega Inspection and Consultancy Limited | Professional & scientific | 2007 |
| Derek a Kirk Properties Ltd | Real estate | 2016 |
| Derek Wilson Welding Services Ltd | Manufacturing | 2024 |
| Devine Property Solutions Ltd | Real estate | 2025 |
| DHR Blades Ltd | Manufacturing | 2025 |
| Diamond Fitness Gym Ltd | Arts & recreation | 2011 |
| Diamonds Bar Ltd | Hospitality | 2011 |
| Diamonds SECURITY1 Ltd | Other services | 2018 |
| Dlma Ltd | Real estate | 2023 |
| Domestic Matters Ltd | Administrative & support | 2024 |
| Donna Anderson Publishing Ltd | Information & communication | 2020 |
| Donnachie Wear Ltd | Wholesale & retail | 2026 |
| Dowie Plumbing & Heating Limited | Construction | 2010 |
| Drainworx Scotland Ltd | Construction | 2025 |
| DS Civil Consulting Ltd | Professional & scientific | 2025 |
| Dual Builders Ltd | Construction | 2020 |
| Duncan-Pryde (Contractors) Limited | Construction | 1979 |
| Dynabright Ltd | Construction | 2013 |
| E & J Douglas & Sons Ltd | Other services | 2009 |
| E & J Douglas Planing & Road Sweeping Ltd | Administrative & support | 2010 |
| Eagle Recycling (UK) Limited | Water & waste | 2004 |
| East Coast Drainage Services Ltd | Water & waste | 2026 |
| East Hydraulic Services Limited | Manufacturing | 2006 |
| Edin Resells Ltd | Wholesale & retail | 2025 |
| Elite Construction & Utility Services Limited | Construction | 2024 |
| EPL Index Limited | Wholesale & retail | 2021 |
| Erin Thain Financial Advice Ltd | Financial services | 2013 |
| Essentials Edge Ltd | Wholesale & retail | 2025 |
| Evolution Motor Group Limited | Wholesale & retail | 2018 |
| Ewens Energy Services Ltd | Construction | 2022 |
| Excel L.P. | | 2013 |
| Fabio's Valli Equipment Ltd | Wholesale & retail | 2019 |
| FABTEK-72 Ltd | Manufacturing | 2026 |
| Falcon Corporation EC Limited | Arts & recreation | 1995 |
| Farrell Building and Construction Ltd | Construction | 2025 |
| Fencing Fife Ltd | Wholesale & retail | 2022 |
| Ferguson and Munro Roofing Ltd | Construction | 2017 |
| Fife Airport Limited | Professional & scientific | 2005 |
| Fife Alarms Ltd | Administrative & support | 2025 |
| Fife Carpet Mill Limited | Wholesale & retail | 2006 |
| Fife Community Law Ltd. | Professional & scientific | 2009 |
| Fife Factors Limited | Real estate | 2007 |
| Fife Heating Solutions Ltd | Construction | 2020 |
| Fife Joinery Ltd. | Construction | 2014 |
| Fife Law Centre | Professional & scientific | 2008 |
| Fife Plumbing & Heating Ltd | Construction | 2021 |
| Fife Youth Sports Academy Ltd | Arts & recreation | 2018 |
| Flame and Heather Limited | Financial services | 2026 |
| Flextrade Ecom Ltd | Wholesale & retail | 2023 |
| Floor Safe Solutions Ltd | Construction | 2012 |
| Flooring by Stuart Blake Limited | Construction | 2026 |
| Flow Couriers Ltd | Transport & logistics | 2020 |
| Flower Study Weddings & Events Ltd | Wholesale & retail | 2024 |
| FMS Drift Prep Ltd | Professional & scientific | 2025 |
| Food a One Ltd | Hospitality | 2024 |
| Foods Paradise Ltd | Wholesale & retail | 2019 |
| Force 1 Foods Limited | Wholesale & retail | 2010 |
| Ford Services Ltd | Construction | 2015 |
| Forth Crop Solutions Ltd. | Agriculture & fishing | 2017 |
| FRD Foods Ltd | Hospitality | 2025 |
| Fried Chicken BAE Ltd | Hospitality | 2022 |
| Friends of Dorothy Ltd | Other services | 2024 |
| FT Solvers Ltd | Wholesale & retail | 2025 |
| Fully Fitted Wardrobes Limited | Manufacturing | 2015 |
| FW Corporation Ltd | Wholesale & retail | 2022 |
| G & E Exterior Cleaning Ltd | Administrative & support | 2025 |
| G & G Nicolson Limited | Construction | 2023 |
| Gary Lambert Ltd | Construction | 2025 |
| Gateway Innovations Limited | Professional & scientific | 2004 |
| GDA Properties Ltd | Real estate | 2023 |
| Gelcolux Ltd | Wholesale & retail | 2023 |
| Gills Deliveries Ltd | Transport & logistics | 2025 |
| Global Havard Ltd | Financial services | 2025 |
| Grattan Rope Access Solutions Limited | Professional & scientific | 2025 |