| Silk Touch Lashes Limited | Other services | 2018 |
| Sjnj Investments Ltd | Real estate | 2023 |
| Slingshot Creative Studios Ltd | Professional & scientific | 2024 |
| SMG Home Improvemts Ltd | Construction | 2026 |
| Snaptek Ltd | Manufacturing | 2013 |
| Software Development AI Limited | Information & communication | 2025 |
| Southfield Properties Limited | Real estate | 2004 |
| Sppug Ltd | Information & communication | 2023 |
| Srodek & Sons Limited | Transport & logistics | 2025 |
| Star Shop 1 Limited | Wholesale & retail | 2025 |
| Stark Butchers Limited | Wholesale & retail | 2011 |
| Starla Collective Ltd | Information & communication | 2025 |
| Starosta Properties Ltd | Real estate | 2025 |
| Stop and Shop Scot Limited | Wholesale & retail | 2026 |
| Strufab Limited | Professional & scientific | 2015 |
| Sumoto Motors Import Ltd | Wholesale & retail | 2025 |
| Sunbabes Limited | Other services | 2014 |
| Surefit Design Limited | Wholesale & retail | 2014 |
| Synex Enterprises Limited | Wholesale & retail | 2025 |
| Tartan Travel Ltd | Transport & logistics | 2025 |
| Tavern and Pubs Ltd | Hospitality | 2025 |
| Taylor Mclean Ltd | Transport & logistics | 2026 |
| Terry,S Tyres Limited | Wholesale & retail | 2004 |
| Text Thought Web Technology Ltd | Wholesale & retail | 2023 |
| THE D.I.C.E Project Limited | Education | 2025 |
| THE East Coast PUB Company Limited | Hospitality | 2004 |
| THE FRY Fifer Ltd | Hospitality | 2025 |
| THE Happy Pet Company Cowdenbeath Limited | Administrative & support | 2019 |
| THE Happy Pet Company Dunfermline Limited | Administrative & support | 2019 |
| THE Happy Pet Company Rosyth Limited | Administrative & support | 2019 |
| THE Modern Private Office Ltd | Administrative & support | 2026 |
| THE Purvis Group Limited | Construction | 2019 |
| THE Village Inn Leisure Limited | Real estate | 2006 |
| THE WEE Diner Ltd. | Hospitality | 2005 |
| Thetradescoach Ltd | Professional & scientific | 2025 |
| Tiger Fitness Ltd | Arts & recreation | 2014 |
| Tikkado's Limited | Hospitality | 2025 |
| Tmta Ltd | Wholesale & retail | 2025 |
| Tony's Appliance Repair Service Ltd | Other services | 2021 |
| Trees in the Mist Ltd | Wholesale & retail | 2024 |
| Tttsmta Ltd | Wholesale & retail | 2025 |
| TW Stonemasons Ltd | Manufacturing | 2021 |
| UN Retail Limited | Hospitality | 2017 |
| Valkarie Games Ltd | Manufacturing | 2023 |
| VIP Travel Scotland Ltd | Transport & logistics | 2022 |
| Vita Solutions UK Ltd | Wholesale & retail | 2019 |
| Vulcan NDT Consultants Limited | Professional & scientific | 2026 |
| W and a Engineering Consultants Limited | Professional & scientific | 2017 |
| Wallsgreen Medical Practice Limited | Health & social care | 2018 |
| Wang's China Chef Ltd | Hospitality | 2024 |
| WEE Ideas Ltd | Information & communication | 2019 |
| Wiecek Ltd | Transport & logistics | 2025 |
| Willdig Dean Ltd | Administrative & support | 2018 |
| Willet Holdings Ltd | Other services | 2024 |
| Wutang Cardenden Limited | Hospitality | 2024 |
| YILING10 Ltd | Hospitality | 2026 |
| Zaib Scot Ltd | Hospitality | 2024 |
| Zel-Us Ltd | Wholesale & retail | 2025 |
| Zeniblend Limited | Administrative & support | 2024 |
| Zodiac School of Dance Ltd | Arts & recreation | 2025 |