| Empteezy Holdings Limited | Financial services | 2022 |
| Empteezy Limited | Manufacturing | 1986 |
| Emres Livingston Ltd | Hospitality | 2020 |
| Enchanted Melts Ltd | Wholesale & retail | 2021 |
| Encorea Ltd | Real estate | 2026 |
| Endurofuel Limited | Wholesale & retail | 2026 |
| Energy Assets Heat Connections Limited | Professional & scientific | 2024 |
| Energy Assets Heat Networks Limited | Professional & scientific | 2020 |
| Energy Assets Heat Solutions Limited | Professional & scientific | 2012 |
| Energy Assets Utilities (Scotland) Limited | Construction | 2003 |
| Engilogic Ltd | Professional & scientific | 2017 |
| Engsaa Ltd | Construction | 2022 |
| Enique IT Solutions Limited | Information & communication | 2018 |
| Enise Hairdresser Limited | Other services | 2021 |
| Ennclick Ltd | Information & communication | 2020 |
| Ennis Consulting Ltd | Professional & scientific | 2016 |
| Entrotec Limited | Manufacturing | 1986 |
| Enviroliance Limited | Professional & scientific | 2004 |
| Environmental Energy Controls EOT Limited | Financial services | 2021 |
| Environmental Energy Controls Ltd | Professional & scientific | 2006 |
| Environmentalhealth CIC | Health & social care | 2019 |
| EPC Scotland Limited | Professional & scientific | 2008 |
| Equinox Electrical Ltd | Energy | 2014 |
| Erradale PM Ltd | Professional & scientific | 2025 |
| Erskine Audio Ltd | Information & communication | 2019 |
| Erskine Software Ltd | Administrative & support | 2017 |
| Ertruck Ltd | Transport & logistics | 2022 |
| Escoces Entertainment Ltd | Information & communication | 2024 |
| Eshaal Limited | Wholesale & retail | 2020 |
| Eska Design Ltd | Wholesale & retail | 2021 |
| Esmail Taxi Co Ltd. | Professional & scientific | 2013 |
| Espace Solutions Ltd | Information & communication | 2020 |
| Estuary System Solutions Ltd | Information & communication | 2024 |
| Ethos Elevate Ltd | Education | 2024 |
| Euan Alexander Training Ltd | Education | 2024 |
| Euguard (Scotland) Limited | Professional & scientific | 2003 |
| Europa Electronics Ltd | Wholesale & retail | 2016 |
| EVE Hearing Limited | Health & social care | 2025 |
| Even That Ltd | Administrative & support | 2024 |
| Event Collection Group Ltd | Administrative & support | 2016 |
| Everfix Limited | Wholesale & retail | 2020 |
| Evergreen Timber Limited | Professional & scientific | 1976 |
| Everything Travel Limited | Wholesale & retail | 2025 |
| Evolution Tkma Ltd | Education | 2018 |
| Evolve My Energy Ltd | Construction | 2019 |
| Ewing Energy Ltd | Manufacturing | 2020 |
| Excel Fire and Security Ltd | Construction | 2019 |
| Exhibit Studios Ltd | Other services | 2024 |
| Express Stitch Limited | Other services | 2017 |
| Express Yourselfie Ltd | Administrative & support | 2020 |
| Exxposed Ltd | Administrative & support | 2022 |
| EZ CAB Ltd | Transport & logistics | 2021 |
| F & F Transport Group Ltd | Transport & logistics | 2025 |
| F & F Transport Ltd | Wholesale & retail | 2021 |
| F & W Westlothian Limited | Hospitality | 2021 |
| F&F Entertainment Limited | Hospitality | 2018 |
| FAB Design Livingston Ltd | Other services | 2025 |
| Fabs Online Student Prep Ltd | Education | 2024 |
| Facade BID Services Ltd | Professional & scientific | 2017 |
| Fadehill Limited | Wholesale & retail | 2025 |
| Fair Price Plumbing Limited | Construction | 2025 |
| Fairbairn Properties Ltd | Real estate | 2024 |
| Fairley Holdings Ltd | Financial services | 2024 |
| Fairo Traders Ltd | Wholesale & retail | 2022 |
| Fairprice Retail Ltd | Wholesale & retail | 2017 |
| Fairway Homes Ltd | Real estate | 2017 |
| Falconer Business Services Ltd | Information & communication | 2011 |
| FAMCO1 Group Limited | Wholesale & retail | 2022 |
| Fantasy Sports Ltd | Information & communication | 2012 |
| Faredeal Cabs Limited | Transport & logistics | 2012 |
| Fashion Love Boutique Ltd | Wholesale & retail | 2025 |
| Fashionnew Pro Limited | Wholesale & retail | 2026 |
| Fast & Clean Craigshill Ltd | Administrative & support | 2023 |
| Fat-Free IT Ltd | Information & communication | 2019 |
| Feam Technical Training (Feamtt) Ltd. | Education | 2021 |
| Ferrier Automotive Ltd | Professional & scientific | 2021 |
| Ferry Marine Limited | Manufacturing | 1985 |
| Ferryfield House Limited | Health & social care | 1995 |
| Festival of Scottish RUM Ltd | Hospitality | 2020 |
| FGA Mechanical Services Ltd | Manufacturing | 2020 |
| Fhirphi Ltd | Professional & scientific | 2026 |
| Fielding Electrical Ltd | Construction | 2024 |
| Fife Property Management Scotland Ltd | Real estate | 2021 |
| Fife Vaper Ltd | Wholesale & retail | 2016 |
| Film Services Ltd | Information & communication | 2012 |
| FINANCE4RENTALS Ltd | Financial services | 2021 |
| Findlay & Co Financial Solutions Ltd | Financial services | 2016 |
| Fine Star UK Ltd | Wholesale & retail | 2013 |
| Finesse Electrical Ltd | Construction | 2016 |
| Finflying Ltd | Wholesale & retail | 2026 |
| FIO Fashions Limited | Wholesale & retail | 2025 |
| Firefly Arts Limited | Arts & recreation | 1990 |
| First Class Nursery Limited | Education | 2005 |
| First Forte Property Investments Ltd. | Real estate | 2006 |
| First Step Robotics CIC | Professional & scientific | 2023 |
| Fitlogic - Training Systems Ltd | Information & communication | 2020 |
| Five Ways Private Ltd | Hospitality | 2024 |
| Fjayp Ltd | Wholesale & retail | 2026 |
| Flannelly Holdings Limited | Financial services | 2016 |
| Flash Motorsports Ltd | Wholesale & retail | 2024 |