| Flavr Collective Ltd | Manufacturing | 2026 |
| Flawless Resin Finishes Ltd | Construction | 2025 |
| FLE Compliance Ltd | Professional & scientific | 2020 |
| FLE Learning Ltd | Information & communication | 2014 |
| Fleming Events & Leisure Limited | Hospitality | 2025 |
| Fletcher Investments LLP | | 2014 |
| Flexi Finds Marketplace Ltd | Wholesale & retail | 2024 |
| Flexible Surface Technology Ltd. | Manufacturing | 1990 |
| Flexmedical Consultants Limited | Professional & scientific | 2017 |
| Flexmedical Solutions Limited | Manufacturing | 2014 |
| Flow Group Properties Limited | Real estate | 2022 |
| Fluidcreative Software Limited | Information & communication | 2018 |
| FLY Buy Limited | Wholesale & retail | 2023 |
| FM1976 Ltd | Hospitality | 2025 |
| FN Healthcare PVT Ltd. | Health & social care | 2019 |
| Fodilicious Limited | Manufacturing | 2017 |
| Food Chain International Limited | Construction | 2024 |
| Food Creations (Bathgate) Limited | Hospitality | 2011 |
| Food GET Go Limited | Hospitality | 2019 |
| Food Options Holdings Limited | Professional & scientific | 2005 |
| Food Options UK Limited | Wholesale & retail | 2002 |
| Football Buzz Ltd | Arts & recreation | 2011 |
| Forbes Catering Services Ltd | Hospitality | 2024 |
| Formu Care Limited | Administrative & support | 2022 |
| Formula 1 Limited | Hospitality | 1994 |
| Forster Residential Ltd | Real estate | 2024 |
| Fortan Outdoors Ltd | Wholesale & retail | 2021 |
| Forth EMC Services Ltd | Professional & scientific | 2017 |
| Forth Mechanical Ltd. | Professional & scientific | 2017 |
| Forth Road Bridge Investments Limited | Administrative & support | 2024 |
| Fortitude Psychology Ltd | Health & social care | 2018 |
| Forys Transport Ltd | Transport & logistics | 2025 |
| FOX Joinery Edinburgh Limited | Construction | 2024 |
| FOX Red Home Services Ltd | Construction | 2019 |
| Foxtek Services Ltd | Information & communication | 2024 |
| Fraheat Ltd | Professional & scientific | 2024 |
| Fraj Ltd | Wholesale & retail | 2025 |
| Fraser Cleaning Technologies Limited | Wholesale & retail | 2002 |
| Fraser LEE Properties Ltd | Real estate | 2023 |
| Fraser Lets Ltd | Real estate | 2025 |
| Fraser-Brown Properties Ltd | Real estate | 2023 |
| Fred's Whisky Limited | Wholesale & retail | 2020 |
| Friendly Cart Ltd | Wholesale & retail | 2025 |
| Front ROW Clothing Limited | Extraterritorial | 2013 |
| FS Connected Ltd | Professional & scientific | 2021 |
| Full Circle Wind Services Ltd. | Manufacturing | 2017 |
| Full Circle Wind Services UK Ltd | Manufacturing | 2010 |
| FUN Panda Hotfood Limited | Hospitality | 2023 |
| Furniture Fuel Ltd | Professional & scientific | 2024 |
| FUZ D Valets Ltd | Administrative & support | 2022 |
| FWR Group Ltd | Wholesale & retail | 2022 |
| Fynstra Limited | Administrative & support | 2025 |
| G & a Real Estate Limited | Real estate | 2023 |
| G&A Livingston Limited | Health & social care | 2025 |
| G&S Eatry Ltd | Hospitality | 2025 |
| G.T.F Films Ltd | Information & communication | 2025 |
| G4FILMS Ltd | Information & communication | 2024 |
| GAC LUX Ltd | Wholesale & retail | 2024 |
| Gafrimex Holdings Limited | Mining & quarrying | 2020 |
| Gaindeh REC Limited | Wholesale & retail | 2019 |
| Galactic Audio Limited | Information & communication | 2020 |
| Galek Ltd | Construction | 2020 |
| Gallis Projects Limited | Construction | 2020 |
| Game Digital Media Limited | Information & communication | 2026 |
| Gamefury Ltd | Wholesale & retail | 2023 |
| Garage Member Limited | Information & communication | 2026 |
| Garden Machinery Scotland Ltd | Manufacturing | 2024 |
| Gardeners Hall Farm Ltd | Financial services | 2024 |
| Gardens R US Scotland Ltd | Administrative & support | 2016 |
| Gary Saunders Plumbing & Heating Limited | Construction | 2026 |
| GAS Safety GUY Ltd | Construction | 2019 |
| Gasglow Ltd. | Construction | 1995 |
| Gb-Properties Group Ltd | Real estate | 2023 |
| Gclivingston Ltd | Hospitality | 2026 |
| GDL - Design & Build Ltd | Construction | 2022 |
| Geeksdane Consultancy Ltd | Information & communication | 2025 |
| Geeksdane Logistics Ltd | Administrative & support | 2026 |
| Gelder Delivers Ltd | Transport & logistics | 2020 |
| GET Fitter Livingston Ltd | Arts & recreation | 2021 |
| Getta Grip Solutions Ltd | Wholesale & retail | 2024 |
| GHA Marketing Ltd | Other services | 2023 |
| Ghaemi Limited | Real estate | 2026 |
| Ghas Group Ltd | Other services | 2023 |
| Ghjl Holdings Ltd | Real estate | 2024 |
| Giggles and More Ltd | Health & social care | 2024 |
| Gilliland E-Learning Services Ltd | Information & communication | 2016 |
| Gilmore and Gilmore Developments Limited | Real estate | 1986 |
| Ginger Mint Letting Solutions Ltd | Real estate | 2025 |
| Gjefitness Ltd | Other services | 2025 |
| GJM Cafés Ltd | Hospitality | 2020 |
| Glasgow North Homecare Ltd | Health & social care | 2024 |
| Glass Glover (Edinburgh) Limited | Professional & scientific | 1949 |
| Glass Gurus Limited | Administrative & support | 2024 |
| Glass's Fruit Markets Limited | Extraterritorial | 1957 |
| Glen Drummond Holdings Ltd | Financial services | 2015 |
| Glen Drummond Ltd | Professional & scientific | 2016 |
| Glen Technology Ltd | Professional & scientific | 2022 |
| Glendale Fencing & Equestrian Services Ltd | Construction | 2021 |
| Glenisla Golf Course Limited | Professional & scientific | 2004 |
| Glenmore Scot Limited | Wholesale & retail | 2025 |