| DM Electrical Limited | Construction | 2016 |
| DMG Biophotonics Limited | Manufacturing | 2014 |
| DNV Consulting Limited | Professional & scientific | 2024 |
| Dobies Heat Centres Ltd. | Wholesale & retail | 1999 |
| Doccom Ltd | Information & communication | 2018 |
| Doggywood Pet Grooming Limited | Other services | 2015 |
| Domuta Ltd | Information & communication | 2021 |
| DON Arancina Ltd | Manufacturing | 2021 |
| Donald Moodie Fishing Ltd. | Agriculture & fishing | 2003 |
| Dorothy Mckinney Ltd | Professional & scientific | 2014 |
| Double TAP Vehicle Cleaning Ltd | Administrative & support | 2025 |
| Dovyconstruction Ltd | Construction | 2016 |
| Downie Limited | Professional & scientific | 2019 |
| Dozie Healthcare Ltd | Health & social care | 2021 |
| DP Speed Ltd | Transport & logistics | 2023 |
| Drinkchain Limited | Hospitality | 2001 |
| Driveexpress Ltd | Transport & logistics | 2025 |
| Drobiazg Ltd | Transport & logistics | 2024 |
| Drumshoreland Bing Ltd | Extraterritorial | 2017 |
| DU Beau Styling Limited | Other services | 2015 |
| Dumgoyne Limited | Health & social care | 2003 |
| DUN Property Limited | Real estate | 2023 |
| Duncan Developments (Building Services) Ltd | Construction | 2017 |
| Duncan Transport Holdings Limited | Other services | 2022 |
| Dundas Building Co. Ltd. | Construction | 1976 |
| Dundas Estates & Development Co. Limited | Manufacturing | 1991 |
| Dundas Estates (Bishopton) Ltd | Construction | 2024 |
| Dundas Estates (Haddington) Ltd | Construction | 2024 |
| Dundas Estates (Shawfair) Ltd | Construction | 2024 |
| Durbadeen Holdings Limited | Manufacturing | 2023 |
| Dutch Developments Ltd | Real estate | 2023 |
| DWA Diving Services Limited | Manufacturing | 2024 |
| DWD Constructions Ltd | Construction | 2022 |
| Dynamicwave Properties Ltd | Real estate | 2025 |
| Dynoworx Limited | Wholesale & retail | 2018 |
| DZD Services Ltd | Professional & scientific | 2017 |
| Dziad Transport Ltd | Transport & logistics | 2021 |
| E & M Horsburgh Limited | Transport & logistics | 2000 |
| E Shoping UK Ltd | Wholesale & retail | 2013 |
| E Zoone UK Ltd | Wholesale & retail | 2019 |
| E.E.A.C. Wood Fuels Ltd | Agriculture & fishing | 2017 |
| Eagle (Scotland) Limited | Wholesale & retail | 1993 |
| Eajb Limited | Real estate | 2025 |
| East and West Lothian Ltd | Wholesale & retail | 2024 |
| East Coast Consultancy Ltd | Professional & scientific | 2008 |
| Eastern Team Ltd | Administrative & support | 2021 |
| Eben Care and Support Ltd | Administrative & support | 2019 |
| Ebermeyer Limited | Other services | 2025 |
| ECE Associates Ltd | Administrative & support | 2010 |
| Ecko Design Ltd | Professional & scientific | 2020 |
| Eco Installations Scotland Ltd | Construction | 2023 |
| Eco Roof Scotland Ltd | Construction | 2024 |
| Eco-Ve Ltd | Wholesale & retail | 2026 |
| Ecoaim Ltd | Professional & scientific | 2026 |
| Ecological Trading Limited | Wholesale & retail | 2002 |
| Ecosse Property Services Ltd | Manufacturing | 2026 |
| Ecotec Heating and Plumbing Engineers Ltd | Construction | 2018 |
| Ecotrait (UK) Ltd | Construction | 2012 |
| Ecstasy Kitchen Limited | Hospitality | 2025 |
| ED TE Ayuda a Crecer Ltd | Professional & scientific | 2025 |
| Edgar Property and Investments Limited | Real estate | 2024 |
| Edin 45 Ltd. | Transport & logistics | 1998 |
| EDIN402 Taxis Ltd. | Professional & scientific | 2004 |
| Edinburgh Bespoke Renovations Ltd | Construction | 2022 |
| Edinburgh Cafe on Wheels Limited | Hospitality | 2021 |
| Edinburgh Coatings T/A Fortwill Auto Paints Ltd | Wholesale & retail | 2019 |
| Edinburgh Fruit Market Limited | Households | 1970 |
| Edinburgh IT Services Ltd | Information & communication | 2025 |
| Edinburgh Organics Wholesale Limited | Wholesale & retail | 2019 |
| Edinburgh Photonics Limited | Manufacturing | 2012 |
| Edinburgh Service Engineers Ltd | Manufacturing | 2011 |
| Edinburgh Travel Solutions Ltd | Transport & logistics | 2024 |
| Edinburgh Windows and Doors Ltd | Manufacturing | 2019 |
| Edinburgh Wines Ltd | Wholesale & retail | 2023 |
| EDP Consulting Engineers Ltd | Administrative & support | 2008 |
| EGO Numb Ltd | Wholesale & retail | 2025 |
| EGO Trip HQ Ltd | Wholesale & retail | 2025 |
| EH54 Ltd | Wholesale & retail | 2016 |
| Elecmech Building Services Ltd | Construction | 2019 |
| Electrify Marketing & Communications Limited | Professional & scientific | 2015 |
| Electro Services Ltd | Construction | 2021 |
| Electromech Industrial Limited | Manufacturing | 2006 |
| Elegant Studio Ltd | Wholesale & retail | 2026 |
| Element Aero Ltd | Professional & scientific | 2020 |
| Elevate Scaffolding Contracts Ltd | Construction | 2026 |
| Eliburn Decor Ltd | Construction | 2019 |
| Elite Chariots Automotive Ltd | Wholesale & retail | 2024 |
| Elite Control Systems Limited | Information & communication | 1992 |
| Elite G L Ltd | Other services | 2017 |
| Elite International Services Ltd | Other services | 2025 |
| Elite Properties Edinburgh Ltd. | Real estate | 2003 |
| Ellen's Glen House Limited | Health & social care | 1998 |
| Elma Property Management Ltd | Real estate | 2023 |
| Emergya Wind Technologies UK Holding Limited | Professional & scientific | 2010 |
| EMF Manufacturing Limited | Manufacturing | 2009 |
| Emibemi Ltd | Transport & logistics | 2024 |
| Emma J Burt Limited | Health & social care | 2023 |
| Emmjam Ltd | Professional & scientific | 2023 |
| Empire IT Consulting Limited | Information & communication | 2022 |
| Empirica Insights Limited | Professional & scientific | 2024 |