| Smart History Limited | Professional & scientific | 2015 |
| Smart Inspectors Ltd | Construction | 2010 |
| SMZ Homes Limited | Real estate | 2024 |
| Solaris Counselling Limited | Administrative & support | 2023 |
| Soutar Properties Limited | Extraterritorial | 2022 |
| Spanishsparkles Ltd | Wholesale & retail | 2024 |
| Spatial Flow Ltd | Professional & scientific | 2014 |
| Spidertech Access Solutions Ltd | Construction | 2018 |
| Spiritpro Limited | Information & communication | 2013 |
| SPS Fife Ltd | Construction | 2020 |
| Sqmc Ltd. | Professional & scientific | 1998 |
| SR Agri Ltd | Wholesale & retail | 2024 |
| SRD Auto Ltd | Manufacturing | 2025 |
| SSI Partnership LLP | | 2019 |
| ST Andrews Harbour Fishing Limited | Agriculture & fishing | 2006 |
| ST Andrews Turfgrass Consultancy Ltd | Professional & scientific | 2010 |
| ST. Andrews Golfing Society | Arts & recreation | 2008 |
| Staffsafety Limited | Professional & scientific | 2000 |
| Staig Construction Ltd | Construction | 2024 |
| Stark Street Contracts Limited | Construction | 2023 |
| Steven Mayes HGV & Agri Services Ltd | Agriculture & fishing | 2021 |
| Stewarding Support Ltd | Administrative & support | 2024 |
| Stewarts Resorts Limited | Hospitality | 2018 |
| Strathclyde Healthcare Ltd | Health & social care | 2023 |
| Surface Solutions (Scotland) Limited | Construction | 2010 |
| Sweet Tooth Food Ltd | Hospitality | 2026 |
| Swilcan Advisors Limited | Administrative & support | 2026 |
| Sword and Sons Ltd | Construction | 2021 |
| Sword Solutions Ltd | Construction | 2025 |
| Swordtech Solutions Ltd | Construction | 2025 |
| Sympa Medical Ltd | Professional & scientific | 2014 |
| Systemize HR Ltd | Administrative & support | 2018 |
| TAH Consulting Ltd | Professional & scientific | 2019 |
| Taspp Trading Limited | Hospitality | 2024 |
| Taste of China Fife Ltd | Hospitality | 2023 |
| Tatlock & Thomson Limited | Professional & scientific | 1995 |
| Taxi Centre (Fife) Ltd | Transport & logistics | 2007 |
| TAY Pearls Ltd | Real estate | 2025 |
| Taylor Made Coach Ltd | Arts & recreation | 2024 |
| Taylor Made Gym & Fitness Limited | Arts & recreation | 2021 |
| Technologies Simplified Ltd | Wholesale & retail | 2025 |
| TGS Inspection Services Ltd | Professional & scientific | 2018 |
| THE Black Partnership LLP | | 2023 |
| THE Bunker Leven Limited | Hospitality | 2025 |
| THE Caddy Academy Limited | Education | 2023 |
| THE Centre for Shamanism Ltd | Education | 2020 |
| THE Cherry on TOP1 Ltd | Wholesale & retail | 2025 |
| THE Corner SA Ltd | Wholesale & retail | 2025 |
| THE Cornershop (SA) Limited | Wholesale & retail | 2018 |
| THE Cupar PUB Company Limited | Wholesale & retail | 2009 |
| THE East Coast Edit Ltd | Wholesale & retail | 2024 |
| THE Eden App Ltd | Professional & scientific | 2025 |
| THE HUT Shop Ltd | Wholesale & retail | 2025 |
| THE Leven Inn Limited | Hospitality | 2024 |
| THE Meganm Method Ltd | Arts & recreation | 2022 |
| THE Muffin MAN Fife Ltd | Wholesale & retail | 2024 |
| THE Mutt Hutt Fife Limited | Other services | 2022 |
| THE Old Brewery Limited | Professional & scientific | 2010 |
| THE Pet Shed Fife Ltd | Wholesale & retail | 2025 |
| THE Unknown Greats Ltd | Information & communication | 2026 |
| THE WEE Couper Tasting Tours Ltd | Administrative & support | 2023 |
| THE Zeitgeist Emporium Ltd | Wholesale & retail | 2019 |
| Thegoodguys Limited | Wholesale & retail | 2009 |
| Therapy Works Scotland Ltd | Health & social care | 2020 |
| Thermal Fluid (Process) Systems Limited | Administrative & support | 2019 |
| Thesoftwareengineers Ltd | Professional & scientific | 2013 |
| Thomson Housing Limited | Real estate | 2022 |
| Thomson Technologies Ltd | Other services | 2022 |
| Thornton Greyhounds Limited | Arts & recreation | 1961 |
| Three Ridings Coffee Ltd | Wholesale & retail | 2019 |
| Tigger & Timmy Ltd | Real estate | 2013 |
| Tina Louise Fisheries Ltd | Wholesale & retail | 2025 |
| TNA Properties Ltd | Real estate | 2017 |
| TOJ Ltd | Wholesale & retail | 2025 |
| Toll Auto Services Ltd | Wholesale & retail | 2016 |
| Top Tier Events Ltd | Administrative & support | 2025 |
| Topknot Joinery Fife Limited | Construction | 2024 |
| Torrie Inns Ltd | Hospitality | 2015 |
| Torrie Taverns (Bonny) Ltd | Hospitality | 2024 |
| Torrie Taverns (Kennoway) Ltd | Hospitality | 2018 |
| Torrie Taverns Ltd | Hospitality | 2015 |
| TR Plumbing & Heating Ltd | Construction | 2016 |
| Trampass Limited | Construction | 2013 |
| Tutoru Limited | Education | 2002 |
| Ultra JET Ltd | Administrative & support | 2026 |
| Unique Physique Ltd. | Health & social care | 2003 |
| Unique Resin Surfacing Ltd | Construction | 2025 |
| VA Construction Services Limited | Construction | 2006 |
| Vectis Consulting Limited | Professional & scientific | 2013 |
| Viking Pest Control Ltd | Administrative & support | 2018 |
| Vintage Tearoom Anstruther Ltd | Hospitality | 2023 |
| VIP Scotland Limited | Administrative & support | 2017 |
| VKH Gordon Ltd | Real estate | 2022 |
| W&H Property Developments Ltd | Construction | 2024 |
| Waffle & Syrup Limited | Hospitality | 2025 |
| Wbfife Ltd | Construction | 2024 |
| WD Bookkeeping and Accountancy Services Ltd | Professional & scientific | 2020 |
| WD Developments Fife Limited | Construction | 2019 |
| Webster Designs Fife Ltd | Manufacturing | 2024 |
| WEE Wishes Limited | Manufacturing | 2022 |