| PLM FAB Ltd | Construction | 2025 |
| Pots to Pipes Ltd | Construction | 2012 |
| Power Painter Ltd | Construction | 2024 |
| Pphp Property Services Limited | Real estate | 2021 |
| PPP Foods Limited | Hospitality | 2025 |
| PR Carpetfitters Ltd | Construction | 2011 |
| Pratis Barns Limited | Other services | 2016 |
| Precision Detail Scotland Ltd | Wholesale & retail | 2024 |
| Prestige Engineering Limited | Professional & scientific | 2005 |
| Prism Engineering (Scotland) Ltd. | Construction | 2012 |
| Process Technology Strategic Consultancy Limited | Manufacturing | 2016 |
| PROJECT3 Homes Ltd | Construction | 2025 |
| Property Guys Limited | Real estate | 2010 |
| Property Links Scotland Ltd | Construction | 2023 |
| Proplinker Ltd | Information & communication | 2026 |
| Puneetpaul Singh Ltd | Hospitality | 2023 |
| QS Insight Ltd | Professional & scientific | 2024 |
| Quayside Self Storage Ltd | Transport & logistics | 2025 |
| R a Carriers Ltd | Transport & logistics | 2015 |
| R M Contractors (Fife) Limited | Manufacturing | 2017 |
| R M Properties (Fife) Ltd | Real estate | 2026 |
| R Maxwell Ltd | Agriculture & fishing | 2012 |
| R T Stuart Holdings Ltd | Financial services | 2024 |
| Radbot Comics Ltd | Information & communication | 2021 |
| Railway Inn (Fife) Ltd | Wholesale & retail | 2016 |
| Ramzan Food Store Lower Methil Limited | Wholesale & retail | 2017 |
| Ramzan Superstore Ltd | Wholesale & retail | 2014 |
| Rathellan Properties Limited | Real estate | 1981 |
| Rayco Developments Limited | Construction | 2003 |
| Rayco Storage Ltd. | Transport & logistics | 2005 |
| Rcsinspection Limited | Professional & scientific | 2024 |
| Real Spice Tandoori Takeaway Ltd | Hospitality | 2025 |
| Red Kite Design (Scotland) Limited | Construction | 2021 |
| Redan Consultancies Ltd | Professional & scientific | 2011 |
| Redpath's Massage Therapy Ltd | Health & social care | 2011 |
| Rehzap Ltd | Real estate | 2024 |
| Renton Law Ltd | Professional & scientific | 2026 |
| Renwick Hamilton Farming Limited | Agriculture & fishing | 2025 |
| Revolution Rentals Ltd | Administrative & support | 2022 |
| Rhand Investments Ltd | Real estate | 2023 |
| RIS (Fife) Limited | Manufacturing | 2013 |
| Rmcinspection Ltd | Professional & scientific | 2024 |
| Rmhi Ltd | Construction | 2024 |
| Robert Summers Transport Limited | Transport & logistics | 1995 |
| Robinson Crusoe (Entitlements) Limited | Agriculture & fishing | 2015 |
| Rosey Mart Ltd | Wholesale & retail | 2025 |
| Rossco Plant Services Ltd | Manufacturing | 2023 |
| RPC Scotland Ltd | Construction | 2006 |
| Rutherford Morison Ltd. | Real estate | 1979 |
| RWB Autotec Ltd | Wholesale & retail | 2018 |
| S a Duncan Limited | Manufacturing | 2018 |
| S Mckinnon Kitchens Ltd | Construction | 2017 |
| S.O Youthful Aesthetics Ltd | Health & social care | 2020 |
| S2C Plumbing & Heating Ltd | Construction | 2023 |
| SA Financial Services (Fife) Ltd. | Financial services | 2009 |
| Sailors Rest Community Gym | Arts & recreation | 2015 |
| Saltire Decorating Services Ltd | Construction | 2026 |
| Saltire Security Limited | Administrative & support | 2017 |
| Sapans 5 Limited | Manufacturing | 2021 |
| Sasu One Ltd | Real estate | 2019 |
| Save Wemyss Ancient Caves Society | Arts & recreation | 2016 |
| SB Services Buckhaven Limited | Manufacturing | 2014 |
| Sceptre Entertainment Ltd | Professional & scientific | 2026 |
| Sceptre Pets and Hobbies Ltd | Wholesale & retail | 2025 |
| Scientific Protection Scotland Limited | Professional & scientific | 2011 |
| Scollin Properties Ltd. | Real estate | 2004 |
| Scot Energy 4 U Limited | Wholesale & retail | 2014 |
| Scot Tech 4 U Limited | Administrative & support | 2025 |
| Scot-Moz Limited | Wholesale & retail | 2025 |
| Scotia Joinery Limited | Construction | 2009 |
| Scotia Property and Rental Ltd | Real estate | 2021 |
| Scotia Sidecars Limited | Manufacturing | 2010 |
| Scott Rafferty Groundworks Ltd | Construction | 2011 |
| Scrl Services Ltd | Construction | 2019 |
| SD Dwellings Limited | Real estate | 2024 |
| Sea Road Fastfood Ltd | Hospitality | 2019 |
| See-Change Optical Services Limited | Real estate | 2004 |
| Serene Streamline Ltd | Transport & logistics | 2026 |
| Serzan Catering Ltd | Hospitality | 2020 |
| SET in Stone Fife Limited | Construction | 2013 |
| SGF Electrical Limited | Construction | 2019 |
| SGS Fife Scotland Limited | Manufacturing | 2025 |
| Shackatan Leven Ltd | Other services | 2023 |
| Shaheen Bangladeshi Restaurant and Takeaway Limited | Hospitality | 2019 |
| Sharleen Ltd | Wholesale & retail | 2024 |
| Shaw Property Investment and Management Services Ltd | Real estate | 2024 |
| Shelby Wootton Ltd | Transport & logistics | 2026 |
| Shining Rival Ltd | Agriculture & fishing | 2023 |
| Shorehead Curry House Limited | Hospitality | 2024 |
| Signature (Fife) Limited | Manufacturing | 2014 |
| Simpspeak Ltd | Wholesale & retail | 2025 |
| Sinclair Watt Architects Limited | Professional & scientific | 2006 |
| SJH Assets Ltd | Professional & scientific | 2018 |
| SJJ Property Services Ltd. | Real estate | 2005 |
| SJJ Self Storage Limited | Transport & logistics | 2018 |
| SK9 Grooming & Fertility Limited | Professional & scientific | 2025 |
| Slag Systems (Glasgow) Limited | Construction | 1964 |
| Sleek Geek Collectibles Ltd | Manufacturing | 2023 |
| Smart Deal World Ltd | Wholesale & retail | 2026 |
| Smart Heritage CIC | Information & communication | 2022 |