| Wellesley Auto Centre Ltd | Wholesale & retail | 2020 |
| Wemyss Harbour (Assets) Limited | Financial services | 2015 |
| Westgate Dundee Limited | Real estate | 2011 |
| WGR & R Ltd | Arts & recreation | 2011 |
| Whatdessert Limited | Hospitality | 2024 |
| WHB (Fife) Ltd | Professional & scientific | 2019 |
| Wild Roots Holdings Limited | Information & communication | 2025 |
| Wild Roots Systems Limited | Information & communication | 2025 |
| Wild Scottish Sauna Designs Ltd | Construction | 2025 |
| Wild Scottish Sauna Ltd | Other services | 2023 |
| Willcam Cranes Ltd | Other services | 2017 |
| William Shand Financial Ltd | Financial services | 2019 |
| Willow & Plum Soap Company Ltd | Manufacturing | 2026 |
| Wiltonican Ltd | Information & communication | 2025 |
| Wind Power Energy Scot Limited | Other services | 2025 |
| Windygates Durievale Bowling and Sports Club | Arts & recreation | 2020 |
| Woodhaven Housing Company Limited | Administrative & support | 2024 |
| Worlds Within Ltd. | Information & communication | 2011 |
| WSW Contracts Limited | Wholesale & retail | 2018 |
| Wully Duck Ltd | Manufacturing | 2018 |
| Wüsch Ltd | Administrative & support | 2025 |
| Xtreme Plumbing & Heating Ltd | Administrative & support | 2018 |
| YES VEE Caterers Ltd | Hospitality | 2026 |
| Yobori INK Limited | Other services | 2016 |
| ZAS786 Ltd | Real estate | 2025 |
| Zedaxis Ltd. | Information & communication | 2009 |
| Zsef Ltd | Wholesale & retail | 2025 |
| Zubi's Lower Largo Limited | Wholesale & retail | 2022 |