| Drainaway Civils Limited | Construction | 2017 |
| Drainaway Services Limited | Construction | 2025 |
| Dramabuds Limited | Education | 2016 |
| Drawing Board Marketing Ltd | Professional & scientific | 2016 |
| DRE Dre's Food Ltd | Wholesale & retail | 2022 |
| Dream Team Dance Academy Ltd | Education | 2022 |
| Drinkall Housing Limited | Real estate | 2022 |
| Drinkwater's Mushrooms Limited | Agriculture & fishing | 1981 |
| Drip Consulting Limited | Health & social care | 2016 |
| Driver-Woodeaves Limited | | 1999 |
| DRK Fashion Ltd | Wholesale & retail | 2018 |
| Dropping Well Village Limited | Hospitality | 1990 |
| DRT Haulage Limited | Transport & logistics | 2025 |
| Drysure Restoration Ltd | Administrative & support | 2025 |
| Dsci Ltd | Information & communication | 2008 |
| DSD Coachbuilders Ltd | Manufacturing | 2019 |
| DTL Development Solutions Limited | Information & communication | 2016 |
| DUE North CIC | Administrative & support | 2024 |
| DUE North Events Ltd. | Administrative & support | 2017 |
| Dukes Play House Limited (the) | Arts & recreation | 1972 |
| Duncan Hall Financial Planning Services Limited | Real estate | 2001 |
| Duncan's Construction Ltd | Construction | 2023 |
| Duncrook Sales Limited | Manufacturing | 2021 |
| Dunmore Homes Limited | Construction | 2023 |
| Dunnow Plant Limited | Agriculture & fishing | 2002 |
| Dust + Scratches Ltd. | Information & communication | 2010 |
| Dvcom Solutions Ltd | Information & communication | 2011 |
| DVK North West Limited | Hospitality | 2026 |
| DW Car Valeting Ltd | Wholesale & retail | 2023 |
| DW Sales Ltd | Administrative & support | 2025 |
| Dynamic Theatre Arts Academy Limited | Arts & recreation | 2026 |
| Dyrnwyn Technology Ltd | Information & communication | 2016 |
| E & a Property Holdings Limited | Real estate | 2022 |
| E & a Trading Limited | Wholesale & retail | 2010 |
| E K Motor Factors Limited | Wholesale & retail | 1965 |
| E T L Property Management Limited | Real estate | 2019 |
| E&M Rentals Limited | Extraterritorial | 2024 |
| E-Radicals Ltd | Wholesale & retail | 2023 |
| E.I Marketing Ltd | Professional & scientific | 2017 |
| E.I. Lancaster Ltd | Wholesale & retail | 2024 |
| E.K Arts Ltd | Administrative & support | 2017 |
| E.K.Brakes(Barrow)Limited | Extraterritorial | 1972 |
| E.K.Brakes(Blackpool)Limited | Extraterritorial | 1968 |
| E2INSPIRE C.I.C. | Health & social care | 2012 |
| EA Associates Limited | Information & communication | 1993 |
| Easyfee Limited | Administrative & support | 2015 |
| Eatsultans Ltd | Hospitality | 2025 |
| EC International Development Ltd | Professional & scientific | 2024 |
| Echemedica Health Ltd | Health & social care | 2026 |
| Eclibris Limited | Information & communication | 2024 |
| Eco Property Development Global Limited | Real estate | 2016 |
| Eco Property Rental and Investments Limited | Real estate | 2024 |
| Eco-Mist Biotechnics Limited | Manufacturing | 2012 |
| Ecomatrix Ltd | Professional & scientific | 2024 |
| Ecommerce Universal Ltd | Wholesale & retail | 2023 |
| Ecotest Limited | Professional & scientific | 2021 |
| Eden Bay Homes Limited | Real estate | 2023 |
| Edge of Time Ltd | Information & communication | 1994 |
| Edmonds Estates Ltd | Real estate | 2022 |
| Edmonds Financial Planning Limited | Financial services | 2017 |
| EEC Lettings Ltd | Real estate | 2024 |
| Egalitarian Publishing Ltd | Information & communication | 2020 |
| Egeria Limited | Information & communication | 2022 |
| EGG Enterprises (North West) Ltd | Information & communication | 2021 |
| EHD North West Limited | Financial services | 2012 |
| Eikon Ltd | Professional & scientific | 2022 |
| Eitr Training Limited | Professional & scientific | 2024 |
| EJS Accountancy Limited | Professional & scientific | 2023 |
| ELA Business Consulting Ltd | Information & communication | 2017 |
| Elaine Pygall School of Dancing Limited | Arts & recreation | 2024 |
| Elaris Consulting Ltd | Professional & scientific | 2025 |
| Elder Grove (Ambleside) Limited | Hospitality | 2024 |
| Eleanor Kate & Co Ltd | Wholesale & retail | 2022 |
| Electech Innovation Cluster Limited | Professional & scientific | 2021 |
| Electri CAL Solutions (NW) Limited | Construction | 2021 |
| Elementary Consultancy Ltd | Information & communication | 2016 |
| Elernion Limited | Hospitality | 2026 |
| Elevate Direction Limited | Other services | 2025 |
| Elevate Educational Psychology Solutions Ltd | Health & social care | 2025 |
| ELIF.12 Ltd | Hospitality | 2024 |
| Elison Ltd | Health & social care | 2016 |
| Elixir Entertainment Ltd | Arts & recreation | 2023 |
| Ellel Holiday Village LLP | | 2017 |
| Ellel Ministries Limited | Real estate | 1989 |
| Ellershaw Build Limited | Construction | 2012 |
| Elliot Properties Limited | Real estate | 2014 |
| Elliott Accounts Ltd | Professional & scientific | 2015 |
| ELM Project Management Services Ltd | Professional & scientific | 2018 |
| Elmhurst Garstang Management Company Ltd | Households | 2006 |
| Elmwood Construction LLP | | 2006 |
| Elmwood Property Investments Limited | Construction | 1999 |
| Elro Products (Lancaster) Limited | Wholesale & retail | 1945 |
| Elswood Limited | Agriculture & fishing | 2015 |
| ELY Hamilton Limited | Administrative & support | 1999 |
| Ember Studios Ltd | Arts & recreation | 2024 |
| Emergency JG Plumbing & Heating Ltd | Construction | 2023 |
| Emma Kate Dance Academy Limited | Arts & recreation | 2023 |
| EMS (North West) Ltd | Manufacturing | 2019 |
| Emues UK CIC | Arts & recreation | 2013 |
| Enablingmnt UK Ltd. | Construction | 2018 |