| Encapsulates Technologies Ltd | Information & communication | 2022 |
| Endomade Ltd | Professional & scientific | 1997 |
| Enes One Company Limited | Hospitality | 2019 |
| ENG Moda Ltd | Other services | 2019 |
| English Hardwood Design Limited | Manufacturing | 1995 |
| ENI Belly Limited | Professional & scientific | 2021 |
| Enigmar Property Limited | Financial services | 2016 |
| ENT Clinic @ Lancaster Limited | Health & social care | 2011 |
| Entec Projects Limited | Construction | 2006 |
| Envirocore Construction Materials Limited | Manufacturing | 2022 |
| Envirofly Consulting (UK) Limited | Professional & scientific | 2013 |
| Environmental Ground Stabilisation Limited | Construction | 2005 |
| Environmental IQ (Holdings) Limited | Water & waste | 2019 |
| Environmental IQ Ltd | Water & waste | 2010 |
| EP Holdings (Lancaster) Limited | Real estate | 2009 |
| Equus Engineering Ltd | Professional & scientific | 2007 |
| Erindale.Xyz Limited | Education | 2023 |
| Errigal Advisory Limited | Professional & scientific | 2005 |
| Ervic Ltd | Real estate | 2025 |
| ESCAL8 Limited | Professional & scientific | 2023 |
| Escape Campus Student Accommodation Ltd | Real estate | 2016 |
| Escendency Limited | Information & communication | 2004 |
| Essa Investments Limited | Real estate | 2020 |
| Essa Property Ltd | Real estate | 2020 |
| Estesjorie Limited | Information & communication | 2025 |
| Eston Homes Limited | Construction | 2022 |
| Eterna Services Limited | Real estate | 2022 |
| Eternal Gardens Limited | Administrative & support | 2004 |
| Ethel & EM Ltd | Wholesale & retail | 2018 |
| ETL Property Developments Limited | Real estate | 2021 |
| ETS Management Limited | Professional & scientific | 2021 |
| EU Collection Limited | Professional & scientific | 1994 |
| Euro Lancaster Ltd | Wholesale & retail | 2025 |
| Euro-Straw Limited | Agriculture & fishing | 2018 |
| Euroclipper Limited | Extraterritorial | 2009 |
| Eurodyne Limited | Professional & scientific | 2001 |
| Europet Express Limited | Transport & logistics | 2026 |
| Ev-Dub Limited | Extraterritorial | 2021 |
| Evans Marine Ltd | Manufacturing | 2023 |
| Evans Marquee Hire Limited | Administrative & support | 2012 |
| Evision Energy Limited | Professional & scientific | 2025 |
| Evolution Apps Limited | Information & communication | 2017 |
| Evolution Care Consultancy Ltd | Public administration | 2024 |
| Evolution Plumbing, Heating & GAS Ltd | Construction | 2022 |
| EVS Cleaning Services Ltd | Other services | 2024 |
| EW Law Ltd | Professional & scientific | 2025 |
| Excalibur (L.V.) Ltd | Professional & scientific | 2004 |
| Exeter Academy of Dance Limited | Arts & recreation | 2025 |
| Expecthour Limited | Households | 1992 |
| Experience EXP Ltd | Professional & scientific | 2013 |
| Express Food to Go Ltd | Hospitality | 2025 |
| Extreme Dance Company Limited | Arts & recreation | 2024 |
| Eyles IS Tyres Limited | Wholesale & retail | 2018 |
| F Dola & Co Ltd | Transport & logistics | 2019 |
| F1.61 Ltd | Manufacturing | 2020 |
| F2F Survey Ltd | Construction | 2018 |
| Fab-Furnishings-Bedlinen Limited | Wholesale & retail | 2013 |
| Faboo News Media Ltd | Information & communication | 2024 |
| Fabrikator Ltd | Professional & scientific | 2023 |
| Facile Solves Ltd | Wholesale & retail | 2024 |
| Facit Quarry Limited | Mining & quarrying | 2003 |
| Faeries Wear Boots CIC | Wholesale & retail | 2026 |
| Fahz Properties Limited | Households | 2023 |
| Faimuz Limited | Health & social care | 2021 |
| Fair Elms Limited | Households | 2003 |
| Fairclough & Woodruff Limited | Construction | 2009 |
| Fairfield Construction and Restoration Ltd | Construction | 2020 |
| Fairfield Performing Arts Royston LLP | | 2023 |
| Fairfield Performing Arts Welwyn-Hatfield LLP | | 2023 |
| Fairway Physio Ltd | Other services | 2018 |
| Fairy Steps Limited | Hospitality | 2017 |
| Faith Electrical Services Limited | Construction | 2024 |
| Falcon Healthcare Ltd | Information & communication | 2022 |
| Falcon Leisure (Morecambe) Limited | Extraterritorial | 2016 |
| Family Home Lancaster Ltd | Real estate | 2020 |
| Faris Apparels Ltd | Wholesale & retail | 2019 |
| Farm Foods (Bury) Limited | Wholesale & retail | 1988 |
| Farm Gate Veterinary Services Limited | Professional & scientific | 2010 |
| Farm Veterinary Consultancy Ltd | Professional & scientific | 2022 |
| Farming Efficiencies Limited | Professional & scientific | 2022 |
| Farrell Heyworth Holdings Limited | Financial services | 2000 |
| Farrell Heyworth Lettings Limited | Real estate | 2001 |
| Farrell Heyworth Limited | Real estate | 1999 |
| Fasa Enter Ltd | Water & waste | 2019 |
| FAT Media Limited | Information & communication | 2009 |
| Faverate Foods Limited | Hospitality | 2020 |
| FAW Consulting Ltd | Professional & scientific | 2026 |
| Fawcett Percolation Services Ltd. | Agriculture & fishing | 2015 |
| Fawcett's Country Sports Limited | Wholesale & retail | 2015 |
| Faye's Barbershop Limited | Other services | 2025 |
| FAZ Track Ltd | Transport & logistics | 2022 |
| FB Engineering Limited | Professional & scientific | 2022 |
| FDA Events Limited | Arts & recreation | 2026 |
| Feizor Holiday Lets Limited | Real estate | 2020 |
| Felix Felicis Ltd | Professional & scientific | 2023 |
| Fembistem Ltd | Health & social care | 2021 |
| Fenchurch Environmental Group Ltd | Extraterritorial | 1991 |
| Fennel TS Limited | Professional & scientific | 2025 |
| Fenton Studios Limited | Real estate | 2012 |
| Fenton Suites Limited | Real estate | 2014 |