| THE Selkie House Ltd. | Health & social care | 2025 |
| THE Shopfit Contractors Ltd | Construction | 2022 |
| THE Sign Company (Scotland) Ltd | Manufacturing | 2005 |
| THE Skelton Corporation Ltd | Professional & scientific | 2025 |
| THE Strathearn Hotel Ltd | Hospitality | 2023 |
| THE Utility GUY Scotland Ltd | Financial services | 2026 |
| THE Van Hire Company (Scotland) Limited | Administrative & support | 2024 |
| THE Venue 13 Ltd | Hospitality | 2025 |
| THE Wedding and Event Rooms Ltd | Wholesale & retail | 2018 |
| THE WEE Paint Shop Limited | Wholesale & retail | 2025 |
| THE X Tractor Company Ltd | Wholesale & retail | 2024 |
| Thedronedad Ltd | Information & communication | 2025 |
| Thegreentravelcompany Limited | Administrative & support | 2023 |
| Thind Private Hire Limited | Transport & logistics | 2013 |
| Thistle Building Services Limited | Construction | 2009 |
| Thistle Care Solutions Limited | Hospitality | 2019 |
| Thistle Waste Fife Ltd | Water & waste | 2023 |
| Thomas Menzies (Builders) Limited | Construction | 1937 |
| Thomas Muir (Waste Management) Limited | Water & waste | 1983 |
| Thomson Fabrications Limited | Manufacturing | 2015 |
| Thornton FRY House Ltd | Hospitality | 2026 |
| Tibel Ltd | Health & social care | 2025 |
| Tide Propulsion Services Ltd | Other services | 2025 |
| Tilestyle Holdings Limited | Financial services | 2005 |
| Timeless Tearooms Ltd | Wholesale & retail | 2017 |
| Tinker-Moon H.S.D. Ltd | Hospitality | 2021 |
| Tipsy Trailer Ltd | Hospitality | 2024 |
| TOC Accountants Admin Ltd | Professional & scientific | 2020 |
| TOC Accountants Ltd | Professional & scientific | 2017 |
| TOC Bookkeeping Limited | Professional & scientific | 2020 |
| TOC Dunnikier Ltd | Professional & scientific | 2023 |
| Tomrok Limited | Transport & logistics | 2020 |
| Torbain 1968 Limited | Extraterritorial | 2014 |
| Torcastle Ltd | Professional & scientific | 2022 |
| Town & Country Tarpave Limited | Extraterritorial | 2013 |
| Trading Tycoons Ltd | Wholesale & retail | 2024 |
| Translate IP Ltd | Professional & scientific | 2017 |
| Transparency Clothing Ltd | Wholesale & retail | 2025 |
| Travair Ltd | Administrative & support | 2025 |
| Travel Your World Limited | Administrative & support | 2015 |
| Treble Twenty Taxis Ltd | Transport & logistics | 2013 |
| Trebor Logistics Ltd | Transport & logistics | 2023 |
| Trevor Harris Plant Repairs Ltd | Manufacturing | 2008 |
| Triangle Holidays Ltd | Administrative & support | 2025 |
| Tristone Construction Ltd | Real estate | 2014 |
| Trod Experts Limited | Wholesale & retail | 2026 |
| Trust in Fife Limited | Health & social care | 1993 |
| TRY Patchwork Ltd | Information & communication | 2019 |
| TU Hair & Beauty Ltd | Other services | 2019 |
| Tullys Hospitality Ltd | Arts & recreation | 2021 |
| Turbo Property Investments Ltd | Real estate | 2026 |
| Turbovend Ltd | Manufacturing | 2026 |
| Turnbull Decorating Limited | Construction | 2024 |
| Tynedale Limited | Real estate | 2014 |
| Tyrie Contracts Limited | Construction | 2024 |
| UAS Perth Ltd | Wholesale & retail | 2019 |
| UK Bike and Sports Company Limited | Manufacturing | 2019 |
| UK Gym TEK Ltd | Manufacturing | 2025 |
| Umar Logistics Ltd | Transport & logistics | 2019 |
| Un:Wynd Fife Ltd | Hospitality | 2026 |
| United Kingdom Hockey CUP Ltd | Arts & recreation | 2025 |
| Unlock Tours Limited | Administrative & support | 2018 |
| Urban Reiver Ltd | Other services | 2016 |
| Urban Summit Apartments Ltd | Real estate | 2024 |
| Ustunfba Ltd | Wholesale & retail | 2025 |
| Uszatki Ltd | Information & communication | 2025 |
| Valentina Holistic Therapy Ltd | Education | 2023 |
| Varshita Traders Ltd | Wholesale & retail | 2025 |
| VCF156 Ltd | Manufacturing | 2026 |
| VCP Holdings Ltd | Financial services | 2020 |
| Vector Infrastructure AI Ltd | Information & communication | 2026 |
| Vendor IQ Limited | Information & communication | 2024 |
| Vendor IQ Technologies Limited | Information & communication | 2024 |
| Vendylabs Ltd | Information & communication | 2026 |
| Venters Property Limited | Real estate | 2021 |
| Venus Kirkcaldy Ltd | Other services | 2026 |
| Verity Financial Planners Ltd | Financial services | 2020 |
| Versatile Asset Management Ltd | Real estate | 2026 |
| Verso Economics Limited | Professional & scientific | 2009 |
| Vertawind Access Ltd | Construction | 2025 |
| Veteran Car Club (Scotland) Limited | Other services | 2002 |
| VGO Ltd | Professional & scientific | 2024 |
| VHQ Ltd | Wholesale & retail | 2026 |
| Vibe Beauty Clinic Limited | Other services | 2014 |
| Victoria Hotel Restaurant Ltd | Hospitality | 2025 |
| Victoria Road Developments Ltd | Real estate | 2025 |
| Villa Prospera Ltd | Real estate | 2013 |
| Vintage Bagpipes Limited | Manufacturing | 2012 |
| Visa Go Australia Ltd | Information & communication | 2017 |
| Vitrumm Ltd | Construction | 2025 |
| Volt Pro Electrical Limited | Administrative & support | 2025 |
| Voltari Limited | Professional & scientific | 2018 |
| VR Theme Park Ltd | Arts & recreation | 2025 |
| W & a Penman LLP | | 2026 |
| W & N Lynch Properties Ltd | Real estate | 2023 |
| W Munro Plumbing & Heating Limited | Construction | 2015 |
| W R Properties (Scotland) Ltd. | Real estate | 2016 |
| W&A Professional Cleaning Services Ltd | Administrative & support | 2023 |
| W888 Ltd | Construction | 2026 |
| Waleed Shahid Ltd | Wholesale & retail | 2024 |