| Staff BUS Limited | Administrative & support | 2025 |
| Stagehire PAY Limited | Manufacturing | 2013 |
| Stahly Quality Foods Limited | Wholesale & retail | 1999 |
| Stahly Quality Foods USA Limited | Professional & scientific | 2006 |
| Stanvale Motors Limited | Wholesale & retail | 1999 |
| Stark's Park Properties Ltd | Real estate | 2002 |
| Steadfast Coaching Ltd | Other services | 2017 |
| Stellar Quines | Arts & recreation | 1995 |
| Steve Curtis Cars Limited | Real estate | 2005 |
| STM Motors Ltd | Wholesale & retail | 2026 |
| Stone & Lime Co Limited | Administrative & support | 2017 |
| Stop and Shop High Street Limited | Wholesale & retail | 2025 |
| Storage Space Glenrothes Ltd | Transport & logistics | 2025 |
| Strata Scotland Ltd. | Agriculture & fishing | 2022 |
| Strathore Holdings Limited | Financial services | 2016 |
| Strathore Plant Hire Limited | Administrative & support | 2002 |
| Strathore Wind Farm Limited | Energy | 2014 |
| Strathtay Property Ltd | Real estate | 2024 |
| Streets Ahead Scotland Ltd | Real estate | 2008 |
| Streetwise Orientation & Navigation Systems Limited | Manufacturing | 2002 |
| Stress & Anxiety Relief Limited | Health & social care | 2020 |
| Styled by K9INE Ltd | Other services | 2021 |
| Styx Glenrothes Ltd | Hospitality | 2022 |
| Styx Kirkcaldy Ltd | Hospitality | 2022 |
| Substances Support Scotland Ltd. | Health & social care | 1986 |
| Success Amanda & Co Ltd | Wholesale & retail | 2021 |
| Summit Engineering Consultants Ltd | Professional & scientific | 2025 |
| Summit-Gate Ltd | Other services | 2019 |
| Sunclubk Ltd | Wholesale & retail | 2026 |
| Sunny Nails & Beauty Limited | Other services | 2024 |
| Sunrise Heights Ltd | Real estate | 2026 |
| Sunshine Developments Scotland Ltd | Financial services | 2014 |
| Sunshine Properties Fife Ltd | Real estate | 1990 |
| Suttie and SON Ltd | Construction | 2021 |
| SW Hardscapes Ltd | Construction | 2022 |
| Swan Car Brokers Ltd | Wholesale & retail | 2024 |
| Swan Dance Academy Ltd | Arts & recreation | 2023 |
| Swimright Limited | Education | 2024 |
| Syed Homes (Scotland) Ltd | Real estate | 2020 |
| Synergy Beauty Training (Scotland) Limited | Education | 2025 |
| T Doubleday Plumbing & Heating Ltd | Construction | 2024 |
| TAI CHI Trading Limited | Wholesale & retail | 2020 |
| Takeeataway Limited | Wholesale & retail | 2017 |
| TAM Mini Express Limited | Wholesale & retail | 2025 |
| Tara Cleave Hairstylist Ltd | Other services | 2019 |
| Tasker Marine Services Ltd | Construction | 2020 |
| Tavish Electrical Limited | Construction | 2012 |
| Taxcafe UK Limited | Information & communication | 2001 |
| Taxi Central Ltd | Transport & logistics | 2003 |
| Taxipad Ltd | Transport & logistics | 2026 |
| Tayem Care Services Ltd | Health & social care | 2023 |
| Tayside Concrete Limited | Transport & logistics | 2000 |
| Tayside Garden Buildings Ltd | Wholesale & retail | 2026 |
| Tbh&B Ltd | Other services | 2024 |
| TC Constructions (Fife) Limited | Construction | 2023 |
| TCD Engineering Ltd | Professional & scientific | 2026 |
| Teach From Home LLP | | 2026 |
| Techcare Design Ltd | Information & communication | 2020 |
| Techconsultant Ltd | Information & communication | 2008 |
| Techlab Fife Ltd | Wholesale & retail | 2019 |
| Technoinstall Limited | Construction | 2015 |
| Tekforce Limited | Construction | 2015 |
| Temple Hall Fastfood and Dessert Ltd | Hospitality | 2025 |
| Templehall Dental Practice Limited | Health & social care | 2016 |
| TG Cleaning Services Ltd | Manufacturing | 2026 |
| That Girl World Limited | Wholesale & retail | 2018 |
| THE Abbotshall (Kirkcaldy) Ltd | Hospitality | 2021 |
| THE Adam Smith Global Foundation | Arts & recreation | 2012 |
| THE Art Cafe at Robertsons Limited | Hospitality | 2015 |
| THE Boiler Scrappage Scheme Limited | Construction | 2009 |
| THE Braw House Company Ltd | Real estate | 2025 |
| THE Building Defects Consultancy Ltd | Professional & scientific | 2019 |
| THE Cheesewives Ltd | Hospitality | 2023 |
| THE Co Collective Ltd | Professional & scientific | 2025 |
| THE Coffee Roasting Project Ltd | Manufacturing | 2018 |
| THE Colloidal Company Ltd | Health & social care | 2016 |
| THE Cottage Dysart Ltd | Real estate | 2021 |
| THE Driveway Company Scotland Ltd | Construction | 2023 |
| THE Enid Hutt Gallery Limited | Wholesale & retail | 2011 |
| THE Farmer's SON Ltd | Wholesale & retail | 2020 |
| THE Flow Collective Ltd | Education | 2024 |
| THE Flyte Group Ltd | Wholesale & retail | 2025 |
| THE Framestoreuk Ltd | Manufacturing | 2022 |
| THE Haunted Screen Ltd | Information & communication | 2024 |
| THE Hive Kirkcaldy | Other services | 2024 |
| THE Holistic Centre Kirkcaldy Ltd | Health & social care | 2007 |
| THE Inn @ Oliver Twistz Ltd | Hospitality | 2025 |
| THE Inverness Pram Centre Limited | Wholesale & retail | 2015 |
| THE Island FRY Ltd | Hospitality | 2024 |
| THE Kilt Route Ltd | Administrative & support | 2025 |
| THE Kingdom of Fife Railway Preservation Society | Arts & recreation | 1992 |
| THE Lead Replacement Company Scotland Ltd | Construction | 2026 |
| THE Nibble Nook Ltd | Hospitality | 2023 |
| THE Old Station ST Andrews Ltd | Hospitality | 2021 |
| THE Orchard Coffee Shop Limited | Hospitality | 2005 |
| THE Outdoor Living Scotland Ltd | Construction | 2021 |
| THE Present Shop (Kirkcaldy) Limited | Wholesale & retail | 2008 |
| THE Present Shop Limited | Wholesale & retail | 1999 |
| THE Roofing Company Scotland Limited | Construction | 2020 |
| THE Secret Training Rooms Ltd | Education | 2024 |