| Walker Property Limited | Real estate | 2016 |
| Wallace Caravans (Kirkcaldy) Limited | Wholesale & retail | 2017 |
| Walsh OR Welsh Limited | Hospitality | 2023 |
| Walter FOX Project Management Limited | Professional & scientific | 2026 |
| Wasim Investments Ltd | Real estate | 2025 |
| Watermint Holdings Ltd | Real estate | 2018 |
| Watsons of Leven Limited | Wholesale & retail | 2014 |
| Waveline Digital Ltd. | Professional & scientific | 2025 |
| Wayne Horton Joinery Ltd | Construction | 2025 |
| WDA Store Ltd | Wholesale & retail | 2025 |
| WE Restore Alloys Limited | Wholesale & retail | 2022 |
| Wellbeing (Keynsham) Limited | Wholesale & retail | 2013 |
| Wellbeing Healthcare Holdings Limited | Administrative & support | 2022 |
| Wellbeing Pharmacies Limited | Wholesale & retail | 2007 |
| Wellbeing Radio Ltd | Information & communication | 2020 |
| Wellbeing Rushport (Middlewich) Limited | Wholesale & retail | 2014 |
| Wemyss Furniture Limited | Manufacturing | 2022 |
| West Brae Developments Limited | Construction | 2025 |
| West Wemyss Community Trust Limited | Other services | 2010 |
| Westlake Analytics Ltd | Information & communication | 2024 |
| Wheatfield Holdings Ltd | Financial services | 2025 |
| Whereverly Limited | Information & communication | 2016 |
| Whitton Fitness Ltd | Arts & recreation | 2022 |
| Wikiwoo Store Ltd | Professional & scientific | 2024 |
| William Crane & SON Ltd | Wholesale & retail | 2010 |
| Williamjamroz Ltd | Transport & logistics | 2024 |
| Willw Ltd | Transport & logistics | 2023 |
| Wilson Gray Flips Ltd | Real estate | 2026 |
| Window Store (Scotland) Ltd. | Wholesale & retail | 2003 |
| Windsor Bespoke Interiors Limited | Construction | 2022 |
| Wine-Md Ltd | Wholesale & retail | 2023 |
| Wing Fung (Fife) Limited | Wholesale & retail | 2010 |
| Wise Plumbing & Heating Limited | Construction | 2014 |
| WKH Surveys Ltd | Professional & scientific | 2023 |
| WM. Anderson Engineering Ltd. | Manufacturing | 2006 |
| WOC Technical Services Ltd | Professional & scientific | 2014 |
| Wolfpack Contracts Limited | Construction | 2025 |
| Womenline Ltd | Other services | 2016 |
| Wonderluv Limited | Administrative & support | 2024 |
| Wood N Garden Timber Buildings Ltd | Wholesale & retail | 2020 |
| Woodlands Van Centre Ltd | Wholesale & retail | 2018 |
| Woodnwire Ltd. | Construction | 2008 |
| Woodside Property Ltd | Hospitality | 2020 |
| Woodstech Limited | Information & communication | 2008 |
| World Wide Warehouse Limited | Real estate | 2004 |
| WR Laing & Co Ltd | Construction | 2024 |
| Wr&P Ltd | Wholesale & retail | 2018 |
| WTS Ventures Ltd | Wholesale & retail | 2026 |
| Wullies Wheels Ltd | Wholesale & retail | 2019 |
| WWH Construction Ltd | Construction | 2021 |
| X-Cabs Limited | Transport & logistics | 2026 |
| X-Hub Ltd | Information & communication | 2025 |
| Yasiman Enterprise CIC | Wholesale & retail | 2026 |
| Yellow CAB Co (Scotland) Ltd | Transport & logistics | 2026 |
| Yellow Moon KDY Ltd | Hospitality | 2017 |
| Young Spuds Ltd | Hospitality | 2020 |
| Young, Hunter and Turnbull Limited | Construction | 2018 |
| Your Builder ( Fife ) Ltd | Construction | 2024 |
| Your Local Roofer (Scotland) Limited | Construction | 2024 |
| Your Local Store Fife Ltd | Wholesale & retail | 2022 |
| Your Roofing Ltd | Construction | 2024 |
| YRC Group Ltd | Hospitality | 2026 |
| YRS 1985 Ltd | Hospitality | 2021 |
| Zaika Cuisine Ltd | Hospitality | 2025 |
| Zains Taxis Limited | Transport & logistics | 2024 |
| Zayka Hayat Ltd | Hospitality | 2025 |
| Zehon Ltd | Wholesale & retail | 2026 |
| ZG Retail Ltd | Wholesale & retail | 2023 |
| ZVR Investments Ltd | Real estate | 2023 |