| Step -UP Supported Living Limited | Health & social care | 2019 |
| Step UP Global Limited | Professional & scientific | 2024 |
| Stepack-X Ltd | Administrative & support | 2021 |
| Stephanie Thomas Coaching Ltd | Education | 2021 |
| Stephen Hemms Aerial Integrity Services Ltd | Professional & scientific | 2023 |
| Stephen Tomkins Racing and Driving Limited | Other services | 2026 |
| Stephens Family Castles Ltd | Administrative & support | 2021 |
| Sterling Plumbing & Heating South West Ltd | Construction | 2026 |
| Sterling Scott Solutions Limited | Extraterritorial | 2018 |
| Sterling Streak Ltd | Wholesale & retail | 2026 |
| Sterlinglink Talent Ltd | Wholesale & retail | 2025 |
| Steve Clarkson Ltd | Hospitality | 2012 |
| Steve Hinton Farrier Ltd | Agriculture & fishing | 2021 |
| Steve House Limited | Construction | 2007 |
| Steve Lyons Transport Ltd | Transport & logistics | 2019 |
| Steve Unett Limited | Construction | 2003 |
| Steve Wood Leisure Ltd | Hospitality | 2018 |
| Steven Gardiner Limited | Construction | 2017 |
| Steven Toon Technical Services Ltd | Manufacturing | 2001 |
| Stevens Family Property Limited | Real estate | 2015 |
| Steveolo Limited | Transport & logistics | 2022 |
| Stewart Golf Limited | Manufacturing | 2003 |
| STF Electrical Services (South West) Ltd. | Construction | 2019 |
| Stfco Property Investment Limited | Real estate | 2024 |
| Stfco Property Management Limited | Real estate | 2024 |
| Stfes Holdings Limited | Real estate | 2023 |
| STI Adhesives Ltd | Manufacturing | 2026 |
| Sticky Web & Digital Ltd | Information & communication | 2024 |
| Stilchester Investments Limited | Financial services | 2024 |
| Still Burning Firewood Ltd | Wholesale & retail | 2022 |
| Stirling Driver Services Limited | Transport & logistics | 2007 |
| STL Gloucester Ltd | Construction | 2021 |
| Stockhome Properties Limited | Real estate | 2008 |
| Stockwell Green Development Ltd | Construction | 2024 |
| Stokes & Sons Security Limited | Administrative & support | 1982 |
| Stokes Carpentry and Building Limited | Construction | 2021 |
| Stokes Welding and Fabrication Limited | Manufacturing | 2005 |
| Stonder UK Ltd | Wholesale & retail | 2025 |
| Stone Barn Fields Management Company Limited | Households | 2016 |
| Stone END Kitchen Limited | Hospitality | 2007 |
| Stoneberry Limited | Real estate | 1996 |
| Stonelea Riding Centre Ltd | Education | 2019 |
| Stonetusk Ltd | Construction | 2021 |
| Stoneway Developments (SW) Ltd | Real estate | 2021 |
| Stoneway Paving and Resin Drives Limited | Construction | 2018 |
| Stookk Ltd | Information & communication | 2020 |
| Storemint Ltd | Wholesale & retail | 2026 |
| Storey Civil and Marine Limited | Construction | 2002 |
| Storm Heating Ltd | Construction | 2014 |
| Stormfinch Limited | Information & communication | 2014 |
| Stormwater Solutions Ltd | Construction | 2022 |
| Stoutz&Co Ltd | Administrative & support | 2026 |
| Stowey Road Ltd | Real estate | 2024 |
| STRAPSWORLD12 Ltd | Wholesale & retail | 2025 |
| Strategic Fleet Consultancy Limited | Professional & scientific | 2024 |
| Strategic Proposals (LGM) Ltd | Professional & scientific | 2011 |
| Stratos Fabrication Limited | Manufacturing | 2014 |
| Stratos Marine Ltd | Manufacturing | 2020 |
| Stratos Precision Engineering Ltd | Manufacturing | 2012 |
| Stratum Construction Limited | Construction | 2006 |
| Stratum Group Holdings Limited | Financial services | 2014 |
| Stratum Plant Limited | Administrative & support | 2014 |
| Stratus Home Finance Ltd | Financial services | 2018 |
| Streak Free Limited | Administrative & support | 2018 |
| Streamshield Limited | Information & communication | 2021 |
| Streetsure Ltd | Construction | 2022 |
| Streetz Ahead Creative Housing Ltd | Health & social care | 2016 |
| Streetz Ahead Property Management Limited | Households | 2018 |
| Strictly Brands Ltd | Extraterritorial | 2021 |
| Stripe Global Services Limited | Financial services | 2007 |
| Striprebelsteakbarleicestersquare Ltd | Hospitality | 2025 |
| Strongbox Investment Ltd | Financial services | 2025 |
| Stroud & District Motor Club Limited | Arts & recreation | 1973 |
| Stroud Book Festival CIC | Arts & recreation | 2017 |
| Stroud Carpet Outlet Limited | Wholesale & retail | 2016 |
| Stroud Phone Repairs Ltd | Wholesale & retail | 2026 |
| Stroud Road Garage Limited | Wholesale & retail | 2014 |
| Stroud Yoga Space Limited | Arts & recreation | 2018 |
| Struma Ltd | Transport & logistics | 2021 |
| Stuart Gardner Financial Services Ltd | Financial services | 2021 |
| Stuart Shackell Ltd | Wholesale & retail | 2021 |
| Stuart Thomas Property Ltd | Real estate | 2024 |
| Student Seats Limited | Transport & logistics | 2022 |
| Studio 20 Bridal Ltd | Wholesale & retail | 2024 |
| Studio Sew.N Ltd | Manufacturing | 2021 |
| Studio Welby Limited | Manufacturing | 2023 |
| Studiofit Gloucester Ltd | Arts & recreation | 2018 |
| Studyo Modern Living Ltd | Wholesale & retail | 2025 |
| Stunt Drive UK Limited | Arts & recreation | 2008 |
| Suaaa (QA) UK Ltd | Real estate | 2025 |
| Suave Property Group Ltd | Real estate | 2026 |
| Success Way Business Limited | Professional & scientific | 2014 |
| SUE Butcher Independent Consultancy Limited | Professional & scientific | 2010 |
| SUE Taylor Limited | Financial services | 2020 |
| SUIT4YOU Ltd | Wholesale & retail | 2024 |
| Sultani Perfumes Ltd | Wholesale & retail | 2024 |
| Sumit Carpentry & Building Ltd | Construction | 2021 |
| Summer Farm Ltd | Wholesale & retail | 2026 |
| Summers Worldwide Travel Limited | Administrative & support | 2020 |
| Summertown Roofing Company Ltd | Construction | 2021 |