| SPR Contractors Limited | Construction | 2006 |
| Spring Environmental Limited | Professional & scientific | 2013 |
| Spring Meadows (Welland) Management Company Limited | Households | 2016 |
| Springbok Engineering UK Ltd | Construction | 2005 |
| Sprout Property Holdings Ltd | Hospitality | 2025 |
| SPS IT Services Ltd | Information & communication | 2018 |
| SPT Property Limited | Real estate | 2018 |
| Spud Buster Ltd | Hospitality | 2025 |
| Spur Networks Ltd | Wholesale & retail | 2016 |
| SPV Newcastle One Limited | Real estate | 2025 |
| SPW Maintenance Ltd | Construction | 2024 |
| Sql-Sales Ltd | Information & communication | 2023 |
| Square Deal (Southwest) Ltd | Wholesale & retail | 2021 |
| Square Management Company (Swindon) Limited | Real estate | 2009 |
| SR Autoworx Ltd | Wholesale & retail | 2020 |
| SR Paradise Limited | Wholesale & retail | 2022 |
| SR Properties Glos Ltd | Households | 2024 |
| Srams D Auto Gloucester Limited | Wholesale & retail | 2024 |
| Srcg (UK) Ltd | Extraterritorial | 2013 |
| Sreeraj Limited | Information & communication | 2025 |
| Sreeranga IT Consultancy Ltd | Information & communication | 2015 |
| SRH Medical Limited | Health & social care | 2014 |
| SRH Medical Services Ltd | Health & social care | 2017 |
| SRI Housing Limited | Real estate | 2024 |
| SRI Info Limited | Information & communication | 2020 |
| SRI Nandan Limited | Real estate | 2024 |
| SRI Sairam Properties Limited | Real estate | 2025 |
| SRI Specialist Cars Ltd | Wholesale & retail | 2026 |
| Srihaas Limited | Other services | 2015 |
| Srijai Limited | Information & communication | 2022 |
| Srikar Consultancy Limited | Information & communication | 2019 |
| Srikrish Limited | Real estate | 2021 |
| Srimatha Limited | Hospitality | 2025 |
| Srinidhi Homes Ltd | Real estate | 2022 |
| Srisan Ltd | Health & social care | 2015 |
| Sriya Hospitality Limited | Wholesale & retail | 2025 |
| SRM Coaching & Mentoring Ltd | Professional & scientific | 2024 |
| Srsl Ltd | Hospitality | 2022 |
| SS Danthuluri Ltd | Professional & scientific | 2019 |
| SS Investors Ltd | Real estate | 2021 |
| Ssab Limited | Health & social care | 2016 |
| Ssaj Ltd | Wholesale & retail | 2019 |
| SSH Systems Limited | Information & communication | 2020 |
| SSJ Group Ltd | Construction | 2017 |
| Sssd Property Limited | Real estate | 2002 |
| ST Aldwyn Garage Ltd | Wholesale & retail | 2012 |
| ST David's SPV Limited | Financial services | 2023 |
| ST Elmo Consultancy Limited | Real estate | 2014 |
| ST James Carpets Limited | Wholesale & retail | 2010 |
| ST James Club (Gloucester) Limited | Hospitality | 2020 |
| ST James Mews Management (Gloucester) Limited | Households | 1990 |
| ST Johns Hill Taverns Ltd | Hospitality | 2019 |
| ST Joseph Best Care Ltd. | Health & social care | 2018 |
| ST Mary's Catholic Primary School, Churchdown | Education | 2011 |
| ST Oswolds View Limited | Real estate | 2021 |
| ST Peter's Catholic High School & Sixth Form Centre | Education | 2011 |
| ST Stephens Road Residents Association Limited | Real estate | 2015 |
| ST Studios Limited | Information & communication | 2017 |
| ST Vincent Property Holdings Ltd | Real estate | 2020 |
| ST Whites Court Committee Ltd | Households | 2006 |
| ST. Anley's Realty Co Ltd. | Hospitality | 1959 |
| ST. Catherine's Court (Gloucester) Limited | Households | 2005 |
| ST. Georges Road Management Company Limited | Real estate | 1976 |
| ST. James Court (Gloucester) Limited | Real estate | 2006 |
| ST. James Trading Estate Limited | Real estate | 1997 |
| ST. Lucies Court Management NO.2 Limited | Households | 1992 |
| ST. Michael (Ashleworth) Management Limited | Real estate | 2001 |
| ST. Michaels Square (Gloucester) Management Limited | Administrative & support | 1986 |
| ST. Swithuns Road (Gloucester) Limited | Households | 2018 |
| St.Augustines Well Management Company Limited | Households | 1999 |
| Staar Girl Limited | Wholesale & retail | 2024 |
| Staff Call Recruitment Specialists Limited | Professional & scientific | 1998 |
| Stage@ Ltd | Arts & recreation | 2017 |
| Stagepay Ltd | Information & communication | 2021 |
| Stait Electrical Services Limited | Construction | 2003 |
| Stallionsvillla Limited | Wholesale & retail | 2023 |
| Stanbridge Accountancy Services Limited | Professional & scientific | 2014 |
| Stanchat Ltd | Public administration | 2025 |
| Stancombe Kitchens & Bathrooms Ltd | Construction | 2025 |
| Standard Brands (UK) Limited | Manufacturing | 2001 |
| Stanley.K Ltd | Transport & logistics | 2022 |
| Stanmira Enterprise UK Ltd | Transport & logistics | 2021 |
| Stansted Logistics Limited | Transport & logistics | 2003 |
| Star Care 24/7 Ltd | Administrative & support | 2021 |
| Star Court Lessees Limited | Households | 1971 |
| Stark Intentions Ltd | Information & communication | 2012 |
| Starlight Tracking Limited | Information & communication | 2019 |
| Starline Care Ltd | Health & social care | 2023 |
| Starmax Food Ltd | Hospitality | 2019 |
| State Care Solutions Limited | Health & social care | 2018 |
| State Clean Solutions Ltd | Administrative & support | 2026 |
| Staunton Developments Limited | Construction | 2015 |
| Staverton Electrical Supplies Ltd | Wholesale & retail | 2010 |
| Staxton Digital Limited | Information & communication | 2022 |
| Steadfast Academy Limited | Education | 2025 |
| Steampunks of Gloucestershire CIC | Arts & recreation | 2025 |
| Steel Armor Legacy Ltd | Manufacturing | 2026 |
| Steel Wolf Scaffolding Ltd | Construction | 2025 |
| Steelspan Structures Limited | Manufacturing | 2025 |
| Steer Direct Limited | Professional & scientific | 2015 |