| Soulnseoul Limited | Hospitality | 2023 |
| Sounds Good 2007 Ltd | Transport & logistics | 2007 |
| Souness Midco Limited | Financial services | 2018 |
| Source Design & Supply Limited | Wholesale & retail | 2024 |
| South Midlands Building & Civil Engineering Limited | Construction | 2017 |
| South Point WQ Management Company Ltd | Real estate | 2025 |
| South Scapes Ltd | Professional & scientific | 2025 |
| South West Arches Limited | Hospitality | 2026 |
| South West Civils Limited | Construction | 2014 |
| South West Compliance Group Limited | Public administration | 2017 |
| South West Compliance Holding Limited | Education | 2025 |
| South West Electrical (Glos) Ltd | Construction | 2021 |
| South West Estates Limited | Real estate | 2018 |
| South West Hair Limited | Other services | 2024 |
| South West Safety Management Ltd | Professional & scientific | 2011 |
| South West Snooker Academy | Extraterritorial | 2009 |
| South West Stone Supplies Limited | Extraterritorial | 2014 |
| South West Tiling Contractors Limited | Construction | 2007 |
| South West Utilities Ltd | Construction | 2021 |
| Southbridge Consulting Services Ltd | Professional & scientific | 2026 |
| Southern Office Services Limited | Professional & scientific | 1985 |
| Southern Quality Solutions Ltd | Professional & scientific | 2014 |
| Southernwood Construction & Developments Limited | Construction | 2020 |
| Southfield House Churchdown Limited | Extraterritorial | 2003 |
| Southfield Maisonettes Limited | Real estate | 1979 |
| Southgate Holdings Ltd | Real estate | 2024 |
| Southgate Investments (Gloucester) Ltd | Real estate | 2017 |
| Southgate Pizza Ltd | Hospitality | 2022 |
| Southgate Street (Gloucester) Management Company Limited | Hospitality | 1986 |
| Southgate Superstore Ltd | Wholesale & retail | 2026 |
| Southgate Wine Stores Ltd | Wholesale & retail | 2025 |
| Southwest Grill Ltd | Hospitality | 2023 |
| Southwest Management Services Limited | Real estate | 2015 |
| Southwest Upvc Limited | Construction | 1992 |
| Sovereign Roofing Specialists Limited | Construction | 2023 |
| Sovereign Software Limited | Manufacturing | 1996 |
| SP Consultancy Gloucester Ltd | Administrative & support | 2024 |
| Spa Caravans Ltd | Wholesale & retail | 2022 |
| Spa Court (Gloucester) Management Limited | Households | 2007 |
| Spa Goint Limited | Health & social care | 2025 |
| Spa Housing UK Ltd | Real estate | 2018 |
| Spa Road Ltd | Real estate | 2019 |
| Spa Road Management Ltd | Households | 2009 |
| Spa Road Properties Limited | Real estate | 2022 |
| Spa Town Property Rentals Limited | Real estate | 1998 |
| Spa Town Rentals LLP | | 2003 |
| Spa Villas Gloucester Ltd | Hospitality | 2023 |
| Spa-Tax Taxis Ltd | Transport & logistics | 2011 |
| Space Bags Ltd | Manufacturing | 2024 |
| Space Centre Self Storage Ltd | Professional & scientific | 2011 |
| Space Centres (Glos) Holdings Limited | Financial services | 2023 |
| Space Centres (Glos) Limited | Transport & logistics | 2006 |
| Space Master (Glos) Limited | Wholesale & retail | 2005 |
| Spaceopen Ltd | Administrative & support | 2024 |
| Spangrenade Limited | Wholesale & retail | 2022 |
| Spark & Shine Car Wash Ltd | Wholesale & retail | 2024 |
| Sparkes Audio Visual Limited | Construction | 2003 |
| Sparkle Creative Ltd | Information & communication | 2014 |
| Sparkled Limited | Manufacturing | 2017 |
| Sparklehive Cleaning Ltd | Administrative & support | 2025 |
| Sparkling Angels Ltd | Education | 2017 |
| Spartan Transport Ltd | Extraterritorial | 2022 |
| Spatial Structures Limited | Construction | 2013 |
| SPE Property Limited | Real estate | 2024 |
| Spec 1 Ltd | Construction | 2023 |
| Spec Commercial Ltd | Construction | 2022 |
| Spec Construction Ltd | Construction | 2020 |
| Spec Group Holdings Ltd | Financial services | 2022 |
| Specialist Rail Developments Ltd | Construction | 2022 |
| Speck Electrical Ltd | Energy | 2025 |
| Spectrum 100 Ltd | Information & communication | 2016 |
| Spectrum Facilities Management Services Limited | Construction | 2016 |
| Spectrum HR Consultancy Limited | Administrative & support | 2020 |
| Spectrum Medical Group Limited | Financial services | 2015 |
| Spectrum Medical International Limited | Wholesale & retail | 2017 |
| Spectrum Medical Limited | Manufacturing | 2006 |
| Spectrum Products (UK) Ltd | Wholesale & retail | 2007 |
| Speedproject Training Limited | Arts & recreation | 2020 |
| Spencer Accounting Services Limited | Professional & scientific | 2003 |
| Spendorsave Ltd | Administrative & support | 2012 |
| Speros Restaurants Ltd | Hospitality | 2017 |
| SPF Logistics Ltd | Transport & logistics | 2024 |
| Spicemantra Ltd | Hospitality | 2024 |
| Spicy Kadai Ltd | Hospitality | 2019 |
| Spiller Fruition Ltd | Information & communication | 2022 |
| Spinnaker Holding Group Limited | Financial services | 2022 |
| Spirit Lane Enterprises Ltd | Manufacturing | 2025 |
| Spiver UK Ltd | Wholesale & retail | 2023 |
| SPJ Aluminium Limited | Construction | 2025 |
| Splash MFP Ltd | Wholesale & retail | 2017 |
| Splendid IT Limited | Information & communication | 2008 |
| Splendid Prints Ltd | Manufacturing | 2025 |
| Splondge Ltd | Professional & scientific | 2023 |
| SPM Consulting Limited | Professional & scientific | 2025 |
| Sport Mentor Network Limited | Education | 2025 |
| Sportbeat 2015 Limited | Hospitality | 2014 |
| Sportily Ltd. | Arts & recreation | 2005 |
| Sports Cupboard Ltd | Wholesale & retail | 2019 |
| Spotty Pots Limited | Wholesale & retail | 2021 |
| SPR Car & Classics Ltd | Wholesale & retail | 2022 |