| ALL in Facilities Management UK Ltd | Construction | 2022 |
| ALL My Friends Childcare Limited | Education | 2006 |
| ALL Nation Foods Ltd | Wholesale & retail | 2020 |
| ALL Nations Community Centre | Other services | 2005 |
| ALL Nations Social Club Ltd | Hospitality | 2019 |
| ALL Nations SV Trading Ltd | Wholesale & retail | 2026 |
| ALL Network Service Solutions Ltd | Construction | 2022 |
| ALL Rail Solutions Limited | Other services | 2015 |
| ALL Saints Court Limited | Households | 2003 |
| ALL Sorts Electrical Solutions Ltd | Construction | 2022 |
| ALL Squared Construction Ltd | Construction | 2024 |
| ALL Things Care Limited | Health & social care | 2025 |
| ALL Web Services Ltd | Information & communication | 2009 |
| Alla&Aksel Ltd | Manufacturing | 2024 |
| Allcooper Property Limited | Real estate | 2015 |
| Allen & Marriott (2026) Limited | Professional & scientific | 2026 |
| Allexi Enterprises Limited | Real estate | 2020 |
| Alleycat Books Ltd | Wholesale & retail | 2025 |
| Alliance Recruitment & Employment Ltd | Administrative & support | 2018 |
| Allied Health Physiotherapy Services Ltd | Health & social care | 2025 |
| Allium Consultancy Limited | Professional & scientific | 2022 |
| Allonby Ltd | Professional & scientific | 2020 |
| Allstone Holdings Limited | Mining & quarrying | 2021 |
| Allstone Sands Gravels Aggregates Trading Co. Ltd. | Mining & quarrying | 1983 |
| Allturnertive Ltd | Real estate | 2011 |
| Allyrhino Clothing Ltd | Wholesale & retail | 2025 |
| Alma House Limited | Real estate | 2007 |
| Almighty Healthcare Ltd | Health & social care | 2020 |
| Almond Limited | Transport & logistics | 2016 |
| Alms Close Apartments Limited | Households | 2013 |
| Alpha Cladding Systems Limited | Wholesale & retail | 2007 |
| Alpha Colour Printers Limited | Manufacturing | 1985 |
| Alpha Fire & Flood Services Limited | Administrative & support | 2011 |
| Alpha Neon Limited | Wholesale & retail | 2016 |
| Alpha Oak Framing Limited | Construction | 2021 |
| Alpha Omega Domiciliary Care Limited | Health & social care | 2017 |
| Alpha Omega Facilities Management Limited | Real estate | 1997 |
| Alpha Responseprint and Mail Limited | Manufacturing | 2004 |
| Alpine Court (Stroud) Limited | Households | 2006 |
| Altaic Ltd | Wholesale & retail | 2023 |
| Alter EGO Apparel Ltd | Wholesale & retail | 2025 |
| Altera Logistics Limited | Wholesale & retail | 2026 |
| Alternate Hypothesis Limited | Information & communication | 2025 |
| Altitude Roofing Gloucestershire Ltd | Construction | 2026 |
| Altorite Ltd | Information & communication | 2024 |
| Altun Kebab Ltd | Hospitality | 2024 |
| Aluminium Extrusion Tooling Co Ltd | Manufacturing | 2019 |
| Aluminium Sashes Limited | Manufacturing | 1976 |
| Always 21 Cheltenham Ltd | Hospitality | 2025 |
| AM & Sons Services Limited | Manufacturing | 2016 |
| AM Products Ltd | Wholesale & retail | 2025 |
| AM Properties Portfolio Ltd | Real estate | 2021 |
| Aman Drylining Ltd | Construction | 2025 |
| Amanah Community CIC | Health & social care | 2023 |
| Amateur Footballer Ltd | Extraterritorial | 2025 |
| Amber Films Limited | Information & communication | 2008 |
| Amber Kelly Limited | Professional & scientific | 2020 |
| Amberley Ridge Management Company Limited | Households | 2018 |
| Ambrotech Ltd | Information & communication | 2025 |
| AMC Mechanical, Heating & Plumbing Ltd | Construction | 2020 |
| AMD Aesthetics Ltd. | Other services | 2023 |
| Amdco Ltd | Financial services | 2019 |
| Amdv Investments Limited | Financial services | 2017 |
| AME Security Installations Ltd | Administrative & support | 2024 |
| Amelie Rose Crafts Ltd | Administrative & support | 2017 |
| Amelio Utilities (Gloucester) Limited | Water & waste | 1999 |
| Ameritech Installations Limited | Construction | 2025 |
| Amfed Limited | Manufacturing | 2024 |
| Amica Advisory Ltd | Professional & scientific | 2021 |
| Amicis Property Limited | Real estate | 2022 |
| Amin Barber Limited | Other services | 2025 |
| Aminu Consultants Ltd | Information & communication | 2021 |
| Amivango Ltd | Transport & logistics | 2026 |
| Amjames Services Limited | Professional & scientific | 2025 |
| Ammonite Paving and Landscaping Ltd | Administrative & support | 2011 |
| Amna Associates Ltd | Other services | 2026 |
| Amor Beauty Cosmetics Ltd | Wholesale & retail | 2018 |
| Amped UP Electrical Services Ltd | Construction | 2009 |
| Amplo Talent Ltd | Administrative & support | 2019 |
| AMR Hold.Co Limited | Financial services | 2026 |
| Amrash Contracting Ltd | Information & communication | 2015 |
| AMS Auctions Ltd | Wholesale & retail | 2013 |
| AMS Fleet Management Holdings Limited | Professional & scientific | 2004 |
| AMS Holdings (Investments) Ltd | Professional & scientific | 2017 |
| AMS Property Gloucestershire Ltd | Real estate | 2024 |
| AN Pollard Property Ltd | Real estate | 2025 |
| Anand Food & Wine Ltd | Wholesale & retail | 2026 |
| Anarion Consulting Services Ltd | Information & communication | 2015 |
| ANB Group Limited | Professional & scientific | 2023 |
| Ancad Ltd | Health & social care | 2022 |
| Anda Communications Ltd | Information & communication | 2020 |
| Anders Properties Limited | Extraterritorial | 2005 |
| Anderson Decorators Limited | Construction | 2025 |
| Anderson Heating Ltd | Construction | 2015 |
| Anderson Recruitment Ltd | Administrative & support | 2011 |
| Andersons Accountancy & Consulting Ltd | Professional & scientific | 2014 |
| Andile Homecare Ltd | Health & social care | 2021 |
| Andover Court Management Company Limited | Households | 2005 |
| Andreea Connect Limited | Information & communication | 2022 |
| Andrew Brown (Technical Services) Ltd | Manufacturing | 2013 |