| AGN Private Ltd | Wholesale & retail | 2023 |
| Agricelerate Global Consulting Limited | Financial services | 2023 |
| Agrigame UK Limited | Agriculture & fishing | 2000 |
| AGS (Gloucester) Limited | Administrative & support | 2023 |
| AGS Capital Limited | Real estate | 2022 |
| AGS Cleaning Services Glos Ltd | Administrative & support | 2025 |
| AGS Property Developments Limited | Construction | 2021 |
| AGV Developments Limited | Construction | 2015 |
| AHC Healthcare Ltd | Health & social care | 2022 |
| Ahlspiess Ltd | Administrative & support | 2018 |
| Ahmati Landscaping Garden Service Ltd | Administrative & support | 2026 |
| Ahsanjaved Ltd | Wholesale & retail | 2025 |
| AI Trading Technologies Limited | Administrative & support | 2021 |
| Aikarthya Ltd | Wholesale & retail | 2025 |
| AIM Medical Solutions Ltd | Wholesale & retail | 2024 |
| AIMA2026 Limited | Hospitality | 2026 |
| Aimek Ltd | Water & waste | 2022 |
| Ainslie Consulting Ltd | Information & communication | 2024 |
| Air Conditioning South West Limited | Construction | 2016 |
| Air Seven Limited | Wholesale & retail | 2011 |
| Air Swiss Ltd | Transport & logistics | 2017 |
| Airae Ltd | Manufacturing | 2025 |
| Aircool Technology Ltd | Construction | 2010 |
| Aires Tech Limited | Professional & scientific | 2016 |
| Airheadz Limited | Wholesale & retail | 2024 |
| Airportsea Taxi Limited | Transport & logistics | 2025 |
| AIS Covertherm Ltd | Manufacturing | 2009 |
| AIS Marine Ltd | Manufacturing | 2013 |
| AIS Subsea Ltd | Manufacturing | 2005 |
| Aitch and Aitch BEE Events Limited | Arts & recreation | 2015 |
| Aiwithabrush Ltd | Wholesale & retail | 2021 |
| AJ Build Limited | Construction | 2020 |
| AJ Carpentry & Joinery Ltd | Manufacturing | 2025 |
| AJ Elite Couriers Ltd | Transport & logistics | 2021 |
| AJ Hooper Investments Ltd | Real estate | 2020 |
| AJ Timbercraft Ltd | Manufacturing | 2024 |
| AJH Agriculture Ltd | Real estate | 2020 |
| AJK Carpentry Limited | Construction | 2006 |
| AJM Mortgages and Financial Services Limited | Financial services | 2026 |
| AJR Electrical Ltd | Construction | 2007 |
| Ajwa Commerce Ltd | Wholesale & retail | 2026 |
| AK Equestrian Ltd | Wholesale & retail | 2024 |
| AK Glos Limited | Hospitality | 2024 |
| AK Innovation Services Ltd | Wholesale & retail | 2025 |
| AK Logistic Services Ltd | Transport & logistics | 2023 |
| AK Smartstaff Ltd | Administrative & support | 2025 |
| AK Systems Ltd | Construction | 2022 |
| AK2 Roofing Ltd | Construction | 2025 |
| Akraj Ventures Limited | Wholesale & retail | 2021 |
| AKS Clark Limited | Construction | 2021 |
| AKS Roofing and Maintenance Ltd | Construction | 2024 |
| Akuarium Limited | Professional & scientific | 2023 |
| Akulaimpex Limited | Wholesale & retail | 2025 |
| Akulun Ltd | Arts & recreation | 2026 |
| AL Buraq Logistics Ltd | Transport & logistics | 2025 |
| AL Furat Mini Market Ltd | Wholesale & retail | 2023 |
| AL Sadiq Ventures Limited | Wholesale & retail | 2026 |
| AL Safaa Ltd | Construction | 2016 |
| Al-Rai Group Ltd | Financial services | 2026 |
| Alagao-Garcia Limited | Health & social care | 2023 |
| Alan Industrial Services Ltd | Wholesale & retail | 2003 |
| Alania Property Ltd | Real estate | 2025 |
| Alasabah Limited | Administrative & support | 2020 |
| Albany Maisonettes (Gloucester) Limited | Households | 1977 |
| Albany Medical Services Ltd | Health & social care | 2025 |
| Albion Developments Cheltenham Ltd | Construction | 2018 |
| Albion Management Group Ltd | Real estate | 2025 |
| Alca Associates Ltd | Real estate | 2023 |
| Alchal LLP | | 2026 |
| Alchemy Alpacas Ltd | Agriculture & fishing | 2019 |
| Alchemy Commercial Ltd | Professional & scientific | 2025 |
| Alchemy Commercial Solutions Ltd | Professional & scientific | 2021 |
| Alchemy Land & Developments Ltd | Construction | 2025 |
| Alchemy Solutions Group Ltd | Financial services | 2025 |
| Alchemy Wellness Limited | Health & social care | 2026 |
| Alco Electrics Limited | Construction | 2022 |
| ALD (Worc's) Properties Ltd | Real estate | 2021 |
| Alden Finance Limited | Financial services | 1983 |
| Aletheia Integrated Management Systems Ltd | Professional & scientific | 2012 |
| Alex Bratan Ltd | Construction | 2018 |
| Alex James Limited | Other services | 2008 |
| Alex Vass Ltd | Arts & recreation | 2021 |
| Alex Whittall Holdings Ltd | Financial services | 2021 |
| Alexander & Angell (Farms) Limited | Agriculture & fishing | 1982 |
| Alexander & Angell Limited | Real estate | 1947 |
| Alexander Hall Farriery Ltd | Agriculture & fishing | 2023 |
| Alexandra Physiotherapy Clinic Limited | Health & social care | 2010 |
| Alfram Limited | Construction | 1993 |
| ALG Gardening & Landscaping Ltd | Administrative & support | 2021 |
| ALI Parker Travel Ltd | Administrative & support | 2023 |
| Alicare (Bristol) Ltd | Health & social care | 2026 |
| Align Health Practice Ltd | Health & social care | 2006 |
| Alison Wright Pictures Ltd | Information & communication | 2014 |
| Alisons Automotive Group Ltd | Wholesale & retail | 2026 |
| Aliyath Property Services Ltd | Real estate | 2021 |
| ALL About Build Ltd | Construction | 2022 |
| ALL About Stuff Ltd | Wholesale & retail | 2021 |
| ALL About Tyres Ltd | Wholesale & retail | 2025 |
| ALL County Trade Limited | Wholesale & retail | 2010 |
| ALL Hub Board Ltd | Professional & scientific | 2022 |