| Andrew Harrison IT Ltd | Information & communication | 2021 |
| Andrew Perry Properties Ltd | Real estate | 2022 |
| Andrew Sparkes Painting and Decorating Service Limited | Construction | 2002 |
| Andrews of Gloucestershire Ltd | Transport & logistics | 2021 |
| Andrews Removals Limited | Transport & logistics | 2011 |
| Andt Plus Ltd | Professional & scientific | 2025 |
| Andy Eagle Investments Ltd | Construction | 2021 |
| Andy LEE Transport Limited | Transport & logistics | 2002 |
| Andy Lewis Performance Limited | Arts & recreation | 2018 |
| Andy Speck Electrical Ltd | Construction | 2011 |
| Aneta 12 Ltd | Administrative & support | 2020 |
| Angad Makeover Limited | Other services | 2025 |
| Angel African Kitchen Limited | Hospitality | 2024 |
| Angel Endoscopy Assist Ltd | Health & social care | 2023 |
| Angell Pets Limited | Wholesale & retail | 2009 |
| Angelvon Properties Ltd | Real estate | 2018 |
| Anham Industries Limited | Mining & quarrying | 2012 |
| Anhui Healthcare Ltd | Health & social care | 2021 |
| Ania the Locum Ltd | Health & social care | 2022 |
| Animal Magic Pet Care (Gloucester) Limited | Education | 2007 |
| Animal Medicines Direct Ltd | Wholesale & retail | 2025 |
| Animals at Home (H/O) Limited | Professional & scientific | 2019 |
| Animals Only Ltd | Wholesale & retail | 2012 |
| Anita Transport Ltd | Transport & logistics | 2021 |
| Anjiru Limited | Professional & scientific | 2005 |
| Anjola Superstore Limited | Wholesale & retail | 2024 |
| Ankris Limited | Transport & logistics | 2020 |
| Anlore Haulage & Plant Ltd | Transport & logistics | 2020 |
| Anlore Limited | Financial services | 2019 |
| Anlore Waste and Recycling Ltd | Transport & logistics | 2020 |
| Anna Hawrot Ltd | Health & social care | 2008 |
| Annabella Yoga Ltd | Health & social care | 2024 |
| Anngie Domestics Services Ltd | Construction | 2024 |
| Annie Joy Ltd | Arts & recreation | 2023 |
| Anns Homes Limited | Real estate | 2026 |
| Anotais Thai Street Food Ltd | Hospitality | 2024 |
| Ansells Carpentry & Renovations Ltd | Manufacturing | 2023 |
| Ansimicro Software (UK) Limited | Information & communication | 2015 |
| Answers in Retirement Limited | Information & communication | 2016 |
| Anthony J Wilkes Livestock Equipment Ltd | Wholesale & retail | 2025 |
| Anthony Millard Consulting Limited | Professional & scientific | 2003 |
| Anthony Systems Consulting Ltd | Professional & scientific | 2026 |
| Anthropics Technology Limited | Information & communication | 1998 |
| Antique Supermarket Limited | Wholesale & retail | 2022 |
| Antomo Property Limited | Real estate | 2021 |
| Anton & K Decorative Antiques and Interiors Limited | Wholesale & retail | 2014 |
| Antony Draper Ltd | Professional & scientific | 2025 |
| Antony Dufort Limited | Arts & recreation | 2009 |
| Anvika Ltd | Wholesale & retail | 2022 |
| Anvil Court Limited | Real estate | 2023 |
| Anvil Point Limited | Information & communication | 2004 |
| Anvil Solutions Limited | Information & communication | 1998 |
| Anybid Limited | Other services | 2026 |
| Anylet Ltd | Real estate | 2022 |
| Anyone CAN Cook Ltd | Education | 2020 |
| AO Mortgages Limited | Financial services | 2019 |
| AOC Flooring Ltd | Wholesale & retail | 2022 |
| AOS Online LLP | | 2009 |
| AOS Security and Recruitment Limited | Administrative & support | 2021 |
| AP Air Europe Ltd | Wholesale & retail | 2010 |
| AP Ground Force Ltd | Professional & scientific | 2024 |
| AP Property Leasing Limited | Real estate | 2023 |
| AP Wealth Limited | Financial services | 2024 |
| Aparajitha Medical Services Limited | Health & social care | 2022 |
| Aparctias Technologies Limited | Other services | 2013 |
| Apartamento Isabel Ltd | Real estate | 2025 |
| APC GAS Services Ltd | Construction | 2012 |
| APC Pest Control Services Ltd | Administrative & support | 2025 |
| Apclarke Properties Ltd | Real estate | 2025 |
| Apex Digital Consulting Ltd | Information & communication | 2021 |
| Apex Events Team Ltd | Arts & recreation | 2021 |
| Apex Heating and Plumbing (Glos) Ltd | Construction | 2019 |
| Apex Heating and Plumbing Ltd | Construction | 2019 |
| Apex One Limited | Construction | 2012 |
| Apex Records Ltd | Information & communication | 2022 |
| Apex Removals Ltd | Transport & logistics | 2025 |
| Apex Residential Lettings Limited | Real estate | 2021 |
| Apex Residential Property Limited | Real estate | 2021 |
| Apex Serene Private Limited | Hospitality | 2026 |
| Apexco Limited | Wholesale & retail | 2016 |
| Apley Automotive Ltd | Wholesale & retail | 2024 |
| APM Bless (UK) Limited | Wholesale & retail | 2017 |
| APM Fire and Security Limited | Administrative & support | 2014 |
| Apollo Radiators Limited | Wholesale & retail | 1997 |
| Apothacarious Ltd | Information & communication | 2025 |
| Appellatus Apparel Ltd | Wholesale & retail | 2025 |
| Applas Worldwide Ltd | Wholesale & retail | 2020 |
| Apple & Oak Ltd | Information & communication | 2018 |
| Applebys Estate Agents Limited | Real estate | 2018 |
| Appletree Communications Limited | Administrative & support | 1992 |
| Applied Bright Steps Therapy Ltd | Education | 2025 |
| Applied Design Systems Limited | | 1986 |
| Applied Rubber Linings Limited | Administrative & support | 1993 |
| Apricot Implant Limited | Professional & scientific | 2025 |
| April Legal Limited | Professional & scientific | 2024 |
| APS Accounts Ltd | Professional & scientific | 2010 |
| APT Group Limited | Other services | 1992 |
| AQM Glos Ltd | Hospitality | 2019 |
| Aqsa Islamic Centre CIC | Other services | 2023 |
| Aqua Bathrooms Direct Limited | Wholesale & retail | 2013 |