| Blackwells Estates Limited | Hospitality | 2025 |
| Bladelaw Limited | Professional & scientific | 2016 |
| Blagden Digital Limited | Information & communication | 2024 |
| Blair Group Holdings Limited | Financial services | 2026 |
| Blairstar Properties Limited | Households | 2024 |
| Blaisdon (Abbeydale) Management Company Limited | Households | 1988 |
| Blake Turnbull Heritage Consultancy Ltd | Professional & scientific | 2025 |
| Blakes Gold and Silver Ltd | Manufacturing | 2023 |
| Blakeway & Hunt Limited | Financial services | 1998 |
| Blakey Prints Limited | Manufacturing | 2025 |
| Blake’S Courier Ltd | Transport & logistics | 2025 |
| Blame IT on Ltd | Administrative & support | 2021 |
| Blanc Strategic Advisory Ltd | Professional & scientific | 2023 |
| Blatchly-Lewis Limited | Professional & scientific | 2020 |
| Blawill Holdings Ltd | Administrative & support | 2015 |
| Blays Surveying Ltd | Administrative & support | 2023 |
| Blending Contractors Limited | Construction | 2016 |
| Blending Property Holdings Ltd | Real estate | 2026 |
| Blenheim House Management (Cheltenham) Limited | Households | 1986 |
| Blink Reservations Limited | Extraterritorial | 2006 |
| BLOCK14MEDIAGROUP Ltd | Manufacturing | 2019 |
| Blondez Ltd | Other services | 2019 |
| Bloom Skin Ltd | Manufacturing | 2025 |
| Bloomai Technologies Limited | Information & communication | 2025 |
| Bloomfield Building Company Ltd. | Construction | 2017 |
| Bloomfield Risk Management Ltd | Financial services | 2008 |
| Bloomsyard Ltd | Hospitality | 2020 |
| Blossoms Properties Ltd | Real estate | 2025 |
| Blouse Limited | Construction | 2015 |
| Bloxham PR Limited | Professional & scientific | 2020 |
| BLS Developments Group Limited | Construction | 2019 |
| Bluarc Fabrications Limited | Manufacturing | 2013 |
| Blue 1 Shop Ltd | Wholesale & retail | 2025 |
| Blue Bamboo Gloucester Limited | Hospitality | 2026 |
| Blue Bubble 3366 Limited | Wholesale & retail | 2023 |
| Blue Caterpillar Learning & Development Ltd | Professional & scientific | 2017 |
| Blue Electric Agency Limited | Arts & recreation | 2026 |
| Blue Flame Digital Solutions Limited | Information & communication | 2003 |
| Blue Fuchsia Ltd | Agriculture & fishing | 2009 |
| Blue Hippo Marketing Ltd | Professional & scientific | 2025 |
| Blue Horizons Limited | Professional & scientific | 2002 |
| Blue Light Aviation Limited | Transport & logistics | 2023 |
| Blue Line Abrasives Limited | Manufacturing | 2023 |
| Blue Matrix Property Investments Limited | Real estate | 2016 |
| Blue Planet Hydrogen Ltd | Professional & scientific | 2009 |
| Blue Sky Signs Ltd | Administrative & support | 2018 |
| Blue Yonder Controls Limited | Manufacturing | 2015 |
| BLUE21 Limited | Professional & scientific | 2011 |
| Bluepeak Labs Limited | Information & communication | 2026 |
| Blueprint Manager Limited | Transport & logistics | 2023 |
| Blueprint Sports Limited | Administrative & support | 2018 |
| Blueray Holdings Limited | Wholesale & retail | 2025 |
| Bluetang UK Ltd | Agriculture & fishing | 2010 |
| Bluetech Digital Ltd | Information & communication | 2024 |
| Bluewold Ltd | Extraterritorial | 2015 |
| Bluprint Developments Ltd | Construction | 2016 |
| Blurred DNB Ltd | Administrative & support | 2022 |
| Blushes Cirencester Limited | Other services | 2020 |
| Blushes Hair Salon Limited | Other services | 2021 |
| Blushes Holdings Limited | Financial services | 2020 |
| Blushes Oxford Limited | Other services | 2014 |
| Blushes Worcester Limited | Other services | 2020 |
| BM 24/7 Recovery Limited | Transport & logistics | 2025 |
| BM Stephens & SON Limited | Agriculture & fishing | 2013 |
| BMC (Cheltenham) Limited | Administrative & support | 2016 |
| BMR Windows Ltd | Construction | 2017 |
| BN European Touring Ltd | Administrative & support | 2026 |
| BNL Property Solutions Limited | Real estate | 2025 |
| BNP Payment Solutions Ltd | Financial services | 2025 |
| BO Holdings Limited | Construction | 2020 |
| Boardwalk Developments Ltd | Administrative & support | 2012 |
| Boathive Ltd | Extraterritorial | 2012 |
| Boats & Homes Ltd | Real estate | 2026 |
| Boats & Homes Ltd Ltd | Hospitality | 2026 |
| Bobster Holdings Limited | Financial services | 2021 |
| Body and Paint Cheltenham Ltd | Wholesale & retail | 2013 |
| Bodyflex UK Limited | Arts & recreation | 2012 |
| Boiswood Ltd | Wholesale & retail | 1989 |
| Bold Corporation Limited | Manufacturing | 2023 |
| Boldr Ltd | Information & communication | 2023 |
| Bollard 15 Co Ltd. | Real estate | 2001 |
| Bollitech Limited | Information & communication | 2010 |
| Bolso Limited | Wholesale & retail | 2025 |
| Bombay Gloucester Ltd | Hospitality | 2024 |
| Bombay MIX Indian Takeaway Ltd | Hospitality | 2025 |
| Bond Brothers Carpentry Limited | Construction | 2014 |
| Bond Brothers Limited | Construction | 2003 |
| Bond Projects Ltd | Professional & scientific | 2018 |
| Bondsgate Estates Limited | Real estate | 2018 |
| Bonham Powell Limited | Professional & scientific | 2015 |
| Bonsa Homes Limited | Real estate | 2018 |
| Bonsa Homes Property Management Limited | Real estate | 2026 |
| Boogie Shoes Ltd | Arts & recreation | 2023 |
| Bookedit Technologies Ltd | Information & communication | 2024 |
| Books and Bits (UK) Limited | Wholesale & retail | 2020 |
| Boom-Buddy Limited | Real estate | 2023 |
| Boost Ventilation Ltd | Wholesale & retail | 2022 |
| Boote Construction Limited | Construction | 2007 |
| Booth & Bomford (Evesham) Limited | Construction | 1960 |
| BOQER123 Ltd. | Wholesale & retail | 2015 |