| Borcom Limited | Information & communication | 2021 |
| Border Consultancy Limited | Construction | 2014 |
| Border Control Services Limited | Administrative & support | 2022 |
| Bore Enterprises Ltd | Hospitality | 2025 |
| Borislav Lyubenov Ltd | Construction | 2025 |
| BOS Leisure Limited | Professional & scientific | 2008 |
| Bosun Care Limited | Health & social care | 2016 |
| Bosun Jack Management Ltd | Administrative & support | 2017 |
| Bottle Green Limited | Extraterritorial | 2007 |
| Boulder in the Stream LLP | | 2025 |
| Boulton Building and Renovations Limited | Construction | 2023 |
| Bounce Hairdressing Ltd | Other services | 2023 |
| Bound (Gloucester) Limited | Financial services | 2014 |
| Boundless Adventure Limited | Arts & recreation | 2025 |
| Bountiful Bees Limited | Agriculture & fishing | 2023 |
| Bountiful Harvest Limited | Wholesale & retail | 2024 |
| Bourne Consultancy Limited | Information & communication | 2024 |
| Bourne Contractors Ltd | Extraterritorial | 2014 |
| Bourne Edge (NO.2) Management Company Limited | Households | 1990 |
| Bourne Electrical Solutions Ltd | Construction | 2023 |
| Bourne to Plumb Ltd | Construction | 2011 |
| Bournemouth Family Care Limited | Health & social care | 2025 |
| Bourton-On-The-Water Goldsmiths Limited | Wholesale & retail | 1998 |
| Bowbrook Construction Limited | Construction | 2024 |
| Bowcare Limited | Wholesale & retail | 2012 |
| Bowcroft Developments Limited | Construction | 2007 |
| Bowden Hall Properties Limited | Real estate | 2015 |
| Bowdens Installations Limited | Construction | 2017 |
| Box & Lock Limited | Transport & logistics | 2022 |
| Box 'a' Surprise Ltd | Wholesale & retail | 2018 |
| Boxappeti Ltd | Hospitality | 2018 |
| Boxkick Limited | Professional & scientific | 2024 |
| Boxlift Ltd | Arts & recreation | 2025 |
| BOY in the Corner Productions Ltd | Information & communication | 2019 |
| Boys 2 MEN Barber Shop Ltd | Other services | 2019 |
| Boznet Ltd | Information & communication | 2023 |
| BP Acquisitions Ltd | Real estate | 2019 |
| BP Builds Ltd | Financial services | 2019 |
| Bpcaccount Ltd | Health & social care | 2025 |
| BPJ ARB Services Ltd | Agriculture & fishing | 2019 |
| BPL (Global) Limited | Transport & logistics | 2007 |
| BPW Solicitors Ltd | Professional & scientific | 2014 |
| BR Accounting Ltd | Professional & scientific | 2018 |
| BR Mentoring Ltd | Education | 2025 |
| Brace Creative Agency Ltd | Information & communication | 2009 |
| Bradbourne House (Gloucester) Limited | Households | 1976 |
| Bradbury Consultancy Limited | Professional & scientific | 2025 |
| Bradburymills Carpentry Ltd | Manufacturing | 2019 |
| Braden Hockley Limited | Construction | 2014 |
| Bradford and Hunnam Studios Limited | Arts & recreation | 2025 |
| Brailsford Golf & Leisure Ltd | Hospitality | 2026 |
| Brains Somerset Ltd | Wholesale & retail | 2023 |
| Brakefield LLP | | 2009 |
| Bramblewood Joinery Ltd | Construction | 2025 |
| Brand Arena Limited | Manufacturing | 2025 |
| Brand Exhibitions Ltd | Administrative & support | 2008 |
| Brand Marketing Studio Ltd | Wholesale & retail | 2017 |
| Brand Picks Limited | Professional & scientific | 2016 |
| Brandize UK Limited | Extraterritorial | 2003 |
| Brandsbyb Ltd | Wholesale & retail | 2023 |
| Brandsfashionfair Limited | Wholesale & retail | 2026 |
| Brasov South Limited | Wholesale & retail | 2020 |
| Brave New Spirits Ltd | Manufacturing | 2020 |
| Bravo (UK) Utils Ltd | Construction | 2024 |
| Bravo Driving School Ltd | Education | 2019 |
| Bravo Golf Limited | Professional & scientific | 2019 |
| Bravura Digital Marketing Ltd | Professional & scientific | 2022 |
| Brdmlk Ltd | Wholesale & retail | 2026 |
| Bread Furniture Limited | Professional & scientific | 2025 |
| Breakthrough Products Limited | Health & social care | 2019 |
| Brearley Management Company Limited | Real estate | 2011 |
| Brecon Stores Limited | Wholesale & retail | 2025 |
| Bredon West Limited | Professional & scientific | 2010 |
| Breese Property & Developments Limited | Construction | 2025 |
| Brendan E. Coles & Associates Limited | Professional & scientific | 1985 |
| Brendan's Bridge | Education | 1989 |
| Brenva Consulting Ltd | Professional & scientific | 2017 |
| Brereton Brickwork Ltd | Construction | 2024 |
| Brett Stuart Limited | Real estate | 2025 |
| Brezewell Limited | Real estate | 2021 |
| BRF Construction Ltd | Construction | 2019 |
| Brian Dickson Consulting Limited | Information & communication | 2015 |
| Brick Project Community Interest Company | Health & social care | 2019 |
| Brick Toy Sets Limited | Wholesale & retail | 2020 |
| Bricks & Moorton Ltd | Construction | 2024 |
| BRICKS2LEARN Ltd | Education | 2024 |
| Brickwood Group Limited | Hospitality | 2023 |
| Bridal Sale Shop Gloucester Ltd | Wholesale & retail | 2014 |
| Bridge Field Court Management Co (Gloucester) Limited | Real estate | 2022 |
| Bridge Training Limited | Education | 2002 |
| Bright Box Electrical Solutions Ltd | Construction | 2023 |
| Bright Environments (Glos) Ltd | Professional & scientific | 2023 |
| Bright Forge Ltd | Wholesale & retail | 2026 |
| Bright Path Mentoring CIC | Education | 2026 |
| Brightdor Limited | Administrative & support | 2025 |
| Brighthaven Healthcare Limited | Health & social care | 2024 |
| Brighton Lawn Care Limited | Administrative & support | 2023 |
| Brightside Healthcare Ltd | Health & social care | 2020 |
| Brightway Edventure Limited | Administrative & support | 2024 |
| Brio Services GL Ltd | Information & communication | 2017 |