| Berserker Spirit Ltd | Professional & scientific | 2024 |
| Bertie BOY at Redmarley Limited | Hospitality | 2025 |
| Bespoke Competition Storage Limited | Wholesale & retail | 2017 |
| Bespoke Mentoring and Training Services Ltd | Professional & scientific | 2018 |
| Bespoke Mentoring Herefordshire Limited | Professional & scientific | 2024 |
| Bespoke Mentoring Worcestershire Ltd | Education | 2024 |
| Best Choice Burgers Ltd | Hospitality | 2025 |
| Best Hand Car Wash 1 Ltd | Administrative & support | 2026 |
| Best Logic Limited | Information & communication | 2018 |
| Best Practice Growth Ltd | Professional & scientific | 2026 |
| Betaris Training Limited | Education | 2011 |
| Betha Care Services Limited | Health & social care | 2020 |
| Bethel Education Limited | Education | 2021 |
| Better Together Recovery & Life Coaching Ltd | Information & communication | 2025 |
| Beulah Healthcare Personnel Ltd | Health & social care | 2022 |
| Beulah Myrtle Properties Ltd | Real estate | 2021 |
| Beverley House Limited | Households | 2002 |
| Bewick House (Gloucester) Limited | Households | 2020 |
| Beyond the Scope Limited | Hospitality | 2018 |
| BG8 Ltd. | Manufacturing | 2017 |
| BG8 Property Ltd | Real estate | 2021 |
| BH Promotions Ltd | Professional & scientific | 2025 |
| BH Race & Repair Ltd | Wholesale & retail | 2025 |
| Bhajis Bristol Road Ltd | Hospitality | 2026 |
| Bham Brothers Ltd | Wholesale & retail | 2011 |
| BHM Developments Limited | Construction | 2006 |
| Biazie UK Ltd | Administrative & support | 2017 |
| Bibury Fish Taxi Ltd | Wholesale & retail | 2023 |
| Bicb Utensils Ltd | Wholesale & retail | 2021 |
| BID4LOTS.COM Ltd | Information & communication | 2004 |
| Bidford Airfield Ltd | Real estate | 1998 |
| Bidmead Property Ltd | Real estate | 2025 |
| Bidnet Ltd | Information & communication | 2018 |
| Big Barworks Clerkenwell Limited | Hospitality | 2025 |
| Big Horse Brands Limited | Wholesale & retail | 2018 |
| Big Punch Studios Limited | Manufacturing | 2015 |
| Bigdug Limited | Wholesale & retail | 2004 |
| Bigfirm Limited | Health & social care | 2014 |
| Bikini Bathrooms Limited | Wholesale & retail | 2004 |
| Bilal HL Limited | Professional & scientific | 2024 |
| Binarytrack Limited | Information & communication | 2012 |
| Binocular Land Limited | Real estate | 2018 |
| Binoyrosehealthcare Ltd | Health & social care | 2026 |
| Biobrainbuddies Ltd | Arts & recreation | 2023 |
| Bioguard Commercial Cleaning Ltd | Administrative & support | 2024 |
| Bionura Limited | Health & social care | 2026 |
| Biostart Security Limited | Administrative & support | 2016 |
| Biostart Trading Limited | Wholesale & retail | 2021 |
| Bira (UK) Limited | Extraterritorial | 2009 |
| Birch Access Ltd | Construction | 2023 |
| Birchams Wood Ltd | Agriculture & fishing | 2022 |
| Birmingham Care Limited | Administrative & support | 2018 |
| Biro Medical Ltd | Manufacturing | 2025 |
| Biscuit TIN Investments Limited | Financial services | 2025 |
| Bishops Holdings (1) Ltd | Real estate | 2024 |
| Bishops Holdings (Gloucester) Ltd | Real estate | 2023 |
| Bishops Holdings (SW) Ltd | Construction | 2023 |
| Bishops Holdings Gloucestershire Ltd | Real estate | 2023 |
| Bisley Farm Shop Limited | Wholesale & retail | 2024 |
| Bisola Care Ltd | Health & social care | 2018 |
| Bitmap Ltd | Information & communication | 2020 |
| Bitrode (UK) Limited | Professional & scientific | 2017 |
| Biznext Solutions Ltd | Information & communication | 2026 |
| BJE Engineering Limited | Professional & scientific | 2008 |
| BJH Accountancy Services Limited | Professional & scientific | 2002 |
| BJK Associates Ltd | Administrative & support | 2017 |
| Bjkc 2 Limited | Extraterritorial | 2023 |
| BJR Consult (UK) Limited | Real estate | 2026 |
| BK Service Company Limited | Administrative & support | 1987 |
| BKH Properties Limited | Real estate | 2009 |
| BKK Social Ltd | Hospitality | 2026 |
| BL ELM1 Limited | Financial services | 2024 |
| BL Glos Property Ltd | Real estate | 2025 |
| BLA Studios Limited | Other services | 2016 |
| Black and White Housing Services Limited | Real estate | 2026 |
| Black Book Cafe Ltd | Hospitality | 2012 |
| Black Box Planning Ltd | Professional & scientific | 2018 |
| Black Diamond AI Ltd | Information & communication | 2025 |
| Black Dragon Computing Limited | Information & communication | 2024 |
| Black HAT Studios Ltd | Wholesale & retail | 2019 |
| Black Hawk Defence Ltd | Public administration | 2026 |
| Black Hill Finco Limited | Administrative & support | 2019 |
| Black Hill Midco Limited | Administrative & support | 2019 |
| Black Hill Topco Limited | Administrative & support | 2019 |
| Black Letter (Gloucester) Ltd | Other services | 2013 |
| Black Letter Studios Limited | Other services | 2014 |
| Black Mango Design Studio Ltd | Professional & scientific | 2025 |
| Black Mesa Research Facility Limited | Real estate | 2022 |
| Black Mountain Developments Ltd | Construction | 2018 |
| Blackbriar Projects Limited | Professional & scientific | 2025 |
| Blackbridge Construction Group Ltd | Construction | 2026 |
| Blackbridge Investments Limited | Real estate | 2026 |
| Blackbridge Recruitment Ltd | Administrative & support | 2024 |
| Blackburn OFC Limited | Arts & recreation | 2023 |
| Blackfriars Wealth Management Ltd | Financial services | 2014 |
| Blackhouse Farm Limited | Agriculture & fishing | 2003 |
| Blackmore Park Unit Co. Limited | Real estate | 2023 |
| Blackmore Trading Park Limited | Real estate | 2023 |
| Blackstock Community Boxing C.I.C. | Arts & recreation | 2026 |
| Blackwells Bookkeeping Ltd | Professional & scientific | 2026 |