| Silverblair Consulting Ltd | Administrative & support | 2018 |
| Silverblair Properties Ltd | Real estate | 2020 |
| Simple Technical Solutions Holdings Limited | Financial services | 2018 |
| Simple Technical Solutions Ltd. | Wholesale & retail | 2002 |
| Simple Technical Solutions Mechanical Engineering Limited | Professional & scientific | 2024 |
| Sips Eco Panel Systems Limited | Manufacturing | 2016 |
| Site CDM Solutions Limited | Professional & scientific | 2014 |
| SJH Property Holdings Ltd | Real estate | 2019 |
| SJM Properties (Fife) Limited | Real estate | 2023 |
| SJW Consult Limited | Professional & scientific | 2026 |
| Skdv Properties (Fife) Limited | Real estate | 2026 |
| Skreenback Limited | Administrative & support | 2019 |
| Sky Parc Limited | Arts & recreation | 2016 |
| Skydive ST. Andrews Limited | Arts & recreation | 2000 |
| Skyline Integrity Solutions Ltd | Professional & scientific | 2024 |
| SL Joiners Limited | Construction | 2015 |
| Slatach Hydro Power Limited | Energy | 2008 |
| Slater Kitchens Ltd | Construction | 2018 |
| SLM Fife Limited | Professional & scientific | 2019 |
| SM Electrical Services (Fife) Ltd. | Construction | 2009 |
| SM Test Solutions Ltd | Professional & scientific | 2020 |
| Smells Like Vintage Ltd | Wholesale & retail | 2022 |
| Smile Support Ltd | Administrative & support | 2024 |
| Smith & Frater Ltd. | Wholesale & retail | 1990 |
| SMK Inspection Limited | Manufacturing | 2011 |
| Smoked Thistle Catering Ltd | Hospitality | 2025 |
| Smooth Air Ventilation Limited | Construction | 2001 |
| Smykker Corp Ltd | Professional & scientific | 2020 |
| SNACKIT7676 Ltd | Hospitality | 2024 |
| Snagging Inspection Scotland Ltd | Construction | 2023 |
| Sntc Ltd | Manufacturing | 2014 |
| Solar Wash Ltd | Administrative & support | 2025 |
| Sonni Services Ltd | Construction | 2024 |
| SOW and Grow Digital Limited | Information & communication | 2023 |
| SP Visionaries Ltd | Households | 2025 |
| Specify Estates Ltd | Real estate | 2017 |
| Specify Ltd | Wholesale & retail | 2013 |
| Spectrogon U.K. Limited | Professional & scientific | 2004 |
| Spectrum Print Marketing Ltd | Other services | 2024 |
| Speedrite Stocktakers Limited | Other services | 2013 |
| Spice of Fife Ltd | Hospitality | 2025 |
| Spms (Scot) Ltd | Financial services | 2019 |
| SR Domestic Cleaning Ltd | Administrative & support | 2025 |
| SRB Bathrooms Ltd | Construction | 2026 |
| SS Construction (Scotland) Ltd | Construction | 2016 |
| SSP Welding Services Ltd | Construction | 2020 |
| ST Andrews Distillers Ltd | Professional & scientific | 2012 |
| ST Andrews Electrical and Isolar Services Limited | Manufacturing | 2018 |
| Stanley Dining Ltd | Hospitality | 2022 |
| Stanley Dining Purchasing Ltd | Hospitality | 2024 |
| Star 5 Holdings Ltd | Construction | 2022 |
| Star Bridal Boutique Ltd | Wholesale & retail | 2019 |
| Star Glamour Ltd | Other services | 2025 |
| Star News (Fife) Ltd | Wholesale & retail | 2013 |
| Star Roofing Limited | Construction | 2003 |
| Starphone & Convenience Store Ltd | Wholesale & retail | 2023 |
| Starscape Café Limited | Hospitality | 2026 |
| Static Scotland Ltd. | Wholesale & retail | 2003 |
| Steadvale Air Systems Limited | Manufacturing | 1992 |
| Steady Built Ltd | Wholesale & retail | 2026 |
| Stephen Contracting Services Ltd | Construction | 2021 |
| Stewart Rankin Agencies Ltd. | Wholesale & retail | 2002 |
| STL Golf Solutions Ltd | Wholesale & retail | 2025 |
| Stockbridge Design and Interiors Limited | Wholesale & retail | 2006 |
| Stonecare (UK) Limited | Manufacturing | 1999 |
| Stonecold Engineering Limited | Professional & scientific | 2026 |
| Storyboard Visuals Ltd | Information & communication | 2026 |
| Strathandco Ltd | Other services | 2026 |
| Strathenry Dairies Fife Limited | Wholesale & retail | 2011 |
| Strathore Garage Scotland Limited | Other services | 2019 |
| Stronachlachar Development Ltd. | Construction | 2017 |
| Struan Watches Ltd | Manufacturing | 2025 |
| Stuart Hamilton Joinery Ltd. | Construction | 2016 |
| Suay Beauty Ltd | Other services | 2019 |
| Sullyz Glenrothes Ltd | Hospitality | 2025 |
| Summers Contracts Ltd | Construction | 2023 |
| Sunhope Packaging Machinery (UK) Ltd | Manufacturing | 2022 |
| Sunlex Global Ltd | Wholesale & retail | 2026 |
| Sunny Skyes Ltd | Wholesale & retail | 2024 |
| Sunnydale Executive Travel Ltd | Transport & logistics | 2024 |
| Super Fade Ltd | Other services | 2024 |
| Superior Shine Cleaning Services Ltd | Administrative & support | 2018 |
| Surcomm Ltd | Information & communication | 2023 |
| Surface Heating Systems Limited | Construction | 1997 |
| Sustainability Fife Limited | Water & waste | 2013 |
| SV Dental Studio Ltd | Health & social care | 2026 |
| Sweetview Developments Limited | Construction | 2021 |
| Swift Building Developments Ltd | Construction | 2025 |
| SWS AA Ltd | Administrative & support | 2023 |
| SWS Global Ltd | Other services | 2025 |
| Syme Holdings Ltd | Manufacturing | 2023 |
| T J Ross (Joiners) Limited | Manufacturing | 1982 |
| T&Am Limited | Real estate | 2022 |
| TAG Properties Scotland Ltd | Real estate | 2024 |
| TAK Mialo BYC Ltd | Real estate | 2025 |
| Take a Load OFF (K) Ltd | Administrative & support | 2023 |
| Talking Horse Design & Print Limited | Professional & scientific | 2021 |
| Tamar Hope Education Ltd | Education | 2024 |
| Tartan Access Solutions Limited | Professional & scientific | 2018 |
| Tartan Tenancies Limited | Real estate | 2024 |